BZW2 (NM_001159767) Human Tagged ORF Clone

CAT#: RC228886

BZW2 (Myc-DDK-tagged)-Human basic leucine zipper and W2 domains 2 (BZW2), transcript variant 1

Product Images

Circular map for RC228886

• 

  Western blot validation of overexpression lysate (Cat# LY431914) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228886 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified BZW2 protein (Cat# TP328886). The protein was produced from HEK293T cells transfected with BZW2 cDNA clone (Cat# RC228886) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BZW2
• Synonyms: HSPC028; MST017; MSTP017
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228886 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAAGCATCAGAAGCCAGTGCTAACAGGCCAGCGGTTCAAAACTCGGAAAAGGGATGAAAAAGAGA
  AATTCGAACCCACAGTCTTCAGGGATACACTTGTCCAGGGGCTTAATGAGGCTGGTGATGACCTTGAAGC
  TGTAGCCAAATTTCTGGACTCTACAGGCTCAAGATTAGATTATCGTCGCTATGCAGACACACTCTTCGAT
  ATCCTGGTGGCTGGCAGTATGCTTGCCCCTGGAGGAACGCGCATAGATGATGGTGACAAGACCAAGATGA
  CCAACCACTGTGTGTTTTCAGCAAATGAAGATCATGAAACCATCCGAAACTATGCTCAGGTCTTCAATAA
  ACTCATCAGGAGATATAAGTATTTGGAGAAGGCATTTGAAGATGAAATGAAAAAGCTTCTCCTCTTCCTT
  AAAGCCTTTTCCGAAACAGAGCAGACAAAGTTGGCGATGCTGTCGGGGATTCTGCTGGGCAATGGCACCC
  TGCCCGCCACCATCCTCACCAGTCTCTTCACCGACAGCTTAGTCAAAGAAGGCATTGCGGCCTCATTTGC
  TGTCAAGCTTTTCAAAGCATGGATGGCAGAAAAAGATGCCAACTCTGTTACCTCGTCTTTGAGAAAAGCC
  AACTTAGACAAGAGGCTGCTTGAACTCTTTCCAGTTAACAGACAGAGTGTGGATCATTTTGCTAAATACT
  TCACTGACGCAGGTCTTAAGGAGCTTTCCGACTTCCTCCGAGTCCAGCAGTCCCTGGGCACCAGGAAGGA
  ACTGCAGAAGGAGCTCCAGGAGCGTCTTTCTCAGGAATGCCCGATCAAGGAGGTGGTGCTTTATGTCAAA
  GAAGAAATGAAGAGGAATGATCTTCCAGAAACAGCAGTGATTGGTCTTCTGTGGACATGTATAATGAACG
  CTGTTGAGTGGAACAAGAAGGAAGAACTTGTTGCAGAGCAGGCTCTGAAGCACCTGAAGCAATATGCTCC
  CCTGCTGGCCGTGTTCAGCTCCCAAGGCCAGTCAGAGCTGATCCTCCTCCAGAAGGTTCAGGAATACTGC
  TACGACAACATCCATTTCATGAAAGCCTTTCAGAAGATTGTGGTTCTCTTTTATAAAGCTGATGTTCTGA
  GCGAAGAAGCAATACTGAAATGGTATAAGGAAGCACATGTTGCTAAAGGCAAAAGTGTTTTTCTTGACCA
  GATGAAGAAATTTGTTGAGTGGTTACAAAATGCAGAAGAAGAATCCGAATCGGAAGGTGAGGAAAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228886 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNKHQKPVLTGQRFKTRKRDEKEKFEPTVFRDTLVQGLNEAGDDLEAVAKFLDSTGSRLDYRRYADTLFD
  ILVAGSMLAPGGTRIDDGDKTKMTNHCVFSANEDHETIRNYAQVFNKLIRRYKYLEKAFEDEMKKLLLFL
  KAFSETEQTKLAMLSGILLGNGTLPATILTSLFTDSLVKEGIAASFAVKLFKAWMAEKDANSVTSSLRKA
  NLDKRLLELFPVNRQSVDHFAKYFTDAGLKELSDFLRVQQSLGTRKELQKELQERLSQECPIKEVVLYVK
  EEMKRNDLPETAVIGLLWTCIMNAVEWNKKEELVAEQALKHLKQYAPLLAVFSSQGQSELILLQKVQEYC
  YDNIHFMKAFQKIVVLFYKADVLSEEAILKWYKEAHVAKGKSVFLDQMKKFVEWLQNAEEESESEGEEN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159767
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159767.1, NP_001153239.1
• RefSeq Size: 1883 bp
• RefSeq ORF: 1260 bp
• Locus ID: 28969
• UniProt ID: Q9Y6E2
• Cytogenetics: 7p21.1
• MW: 48.2 kDa
• Gene Summary: May be involved in neuronal differentiation.[UniProtKB/Swiss-Prot Function]