HSPC302 (TBCK) (NM_001163435) Human Tagged ORF Clone

CAT#: RC228610

TBCK (Myc-DDK-tagged)-Human TBC1 domain containing kinase (TBCK), transcript variant 1

Product Images

Circular map for RC228610

• 

  Western blot validation of overexpression lysate (Cat# LY431639) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228611 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HSPC302
• Synonyms: HSPC302; IHPRF3; TBCKL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228610 representing NM_001163435
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCCCCTGAAGGACGCTGAAATGGGAGCCTTTACCTTCTTTGCCTCGGCTCTGCCACATGATGTTT
  GTGGAAGCAATGGACTTCCTCTCACACCAAATTCCATCAAAATTTTAGGGCGCTTTCAAATCCTTAAAAC
  CATCACCCATCCCAGACTCTGCCAGTATGTGGATATTTCTAGGGGAAAGCATGAACGACTAGTGGTCGTG
  GCTGAACATTGTGAACGTAGTCTGGAAGACTTGCTTCGAGAAAGGAAACCTGTGAGCTGTTCAACGGTTT
  TGTGTATAGCATTTGAGGTTCTTCAGGGCTTGCAGTATATGAACAAACATGGTATAGTACACAGGGCATT
  GTCTCCTCATAATATCCTGTTGGACCGAAAGGGACATATTAAATTGGCTAAATTTGGACTTTATCACATG
  ACAGCTCATGGTGATGATGTTGATTTCCCAATAGGGTATCCCTCGTACTTGGCCCCTGAGGTAATTGCAC
  AGGGAATTTTCAAAACCACTGATCACATGCCAAGTAAAAAACCATTGCCTTCTGGCCCCAAATCAGATGT
  ATGGTCTCTTGGAATCATTTTATTTGAGCTTTGTGTGGGAAGAAAATTATTTCAGAGCTTGGATATTTCT
  GAAAGACTAAAATTTTTGCTTACTTTGGATTGTGTAGATGACACTTTAATAGTTCTGGCTGAAGAGCATG
  GTTGTTTGGACATTATAAAGGAGCTTCCTGAAACTGTGATAGATCTTTTGAATAAGTGCCTTACCTTCCA
  TCCTTCTAAGAGGCCAACCCCAGATGAATTAATGAAGGACAAAGTATTCAGTGAGGTATCACCTTTATAT
  ACCCCCTTTACCAAACCTGCCAGTCTGTTTTCATCTTCTCTGAGATGTGCTGATTTAACTCTGCCTGAGG
  ATATCAGTCAGTTGTGTAAAGATATAAATAATGATTACCTGGCAGAAAGATCTATTGAAGAAGTGTATTA
  CCTTTGGTGTTTGGCTGGAGGTGACTTGGAGAAAGAGCTTGTCAACAAGGAAATCATTCGATCCAAACCA
  CCTATCTGCACACTCCCCAATTTTCTCTTTGAGGATGGTGAAAGCTTTGGACAAGGTCGAGATAGAAGCT
  CGCTTTTAGATGATACCACTGTGACATTGTCGTTATGCCAGCTAAGAAATAGATTGAAAGATGTTGGTGG
  AGAAGCATTTTACCCATTACTTGAAGATGACCAGTCTAATTTACCTCATTCAAACAGCAATAATGAGTTG
  TCTGCAGCTGCCACGCTCCCTTTAATCATCAGAGAGAAGGATACAGAGTACCAACTAAATAGAATTATTC
  TCTTCGACAGGCTGCTAAAGGCTTATCCATATAAAAAAAACCAAATCTGGAAAGAAGCAAGAGTTGACAT
  TCCTCCTCTTATGAGAGGTTTAACCTGGGCTGCTCTTCTGGGAGTTGAGGGAGCTATTCATGCCAAGTAC
  GATGCAATTGATAAAGACACTCCAATTCCTACAGATAGACAAATTGAAGTGGATATTCCTCGCTGTCATC
  AGTACGATGAACTGTTATCATCACCAGAAGGTCATGCAAAATTTAGGCGTGTATTAAAAGCCTGGGTAGT
  GTCTCATCCTGATCTTGTGTATTGGCAAGGTCTTGACTCACTTTGTGCTCCATTCCTATATCTAAACTTC
  AATAATGAAGCCTTGGCTTATGCATGTATGTCTGCTTTTATTCCCAAATACCTGTATAACTTCTTCTTAA
  AAGACAACTCACATGTAATACAAGAGTATCTGACTGTCTTCTCTCAGATGATTGCATTTCATGATCCAGA
  GCTGAGTAATCATCTCAATGAGATTGGTTTCATTCCAGATCTCTATGCCATCCCTTGGTTTCTTACCATG
  TTTACTCATGTATTTCCACTACACAAAATTTTCCACCTCTGGGATACCTTACTACTTGGGAATTCCTCTT
  TCCCATTCTGTATTGGAGTAGCAATTCTTCAGCAGCTGCGGGACCGGCTTTTGGCTAATGGCTTTAATGA
  GTGTATTCTTCTCTTCTCCGATTTACCAGAAATTGACATTGAACGCTGTGTGAGAGAATCTATCAACCTG
  TTTTGTTGGACTCCTAAAAGTGCTACTTACAGACAGCATGCTCAACCTCCAAAGCCATCTTCTGACAGCA
  GTGGAGGCAGAAGTTCGGCACCTTATTTCTCTGCTGAGTGTCCAGATCCTCCAAAGACAGATCTGTCAAG
  AGAATCCATCCCATTAAATGACCTGAAGTCAGAAGTATCACCACGGATTTCAGCAGAGGACCTGATTGAC
  TTGTGTGAGCTCACAGTGACAGGCCACTTCAAAACACCCAGCAAGAAAACAAAGTCCAGTAAACCAAAGC
  TCCTGGTGGTTGACATCCGGAATAGTGAAGACTTTATTCGTGGTCACATTTCAGGAAGCATCAACATTCC
  ATTCAGTGCTGCCTTCACTGCAGAAGGGGAGCTTACCCAGGGCCCTTACACTGCTATGCTCCAGAACTTC
  AAAGGGAAGGTCATTGTCATCGTGGGGCATGTGGCAAAACACACAGCTGAGTTTGCAGCTCACCTTGTGA
  AGATGAAATATCCAAGAATCTGTATTCTAGATGGTGGCATTAATAAAATAAAGCCAACAGGCCTCCTCAC
  CATCCCATCTCCTCAAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228610 representing NM_001163435
  Red=Cloning site Green=Tags(s)
  
  MFPLKDAEMGAFTFFASALPHDVCGSNGLPLTPNSIKILGRFQILKTITHPRLCQYVDISRGKHERLVVV
  AEHCERSLEDLLRERKPVSCSTVLCIAFEVLQGLQYMNKHGIVHRALSPHNILLDRKGHIKLAKFGLYHM
  TAHGDDVDFPIGYPSYLAPEVIAQGIFKTTDHMPSKKPLPSGPKSDVWSLGIILFELCVGRKLFQSLDIS
  ERLKFLLTLDCVDDTLIVLAEEHGCLDIIKELPETVIDLLNKCLTFHPSKRPTPDELMKDKVFSEVSPLY
  TPFTKPASLFSSSLRCADLTLPEDISQLCKDINNDYLAERSIEEVYYLWCLAGGDLEKELVNKEIIRSKP
  PICTLPNFLFEDGESFGQGRDRSSLLDDTTVTLSLCQLRNRLKDVGGEAFYPLLEDDQSNLPHSNSNNEL
  SAAATLPLIIREKDTEYQLNRIILFDRLLKAYPYKKNQIWKEARVDIPPLMRGLTWAALLGVEGAIHAKY
  DAIDKDTPIPTDRQIEVDIPRCHQYDELLSSPEGHAKFRRVLKAWVVSHPDLVYWQGLDSLCAPFLYLNF
  NNEALAYACMSAFIPKYLYNFFLKDNSHVIQEYLTVFSQMIAFHDPELSNHLNEIGFIPDLYAIPWFLTM
  FTHVFPLHKIFHLWDTLLLGNSSFPFCIGVAILQQLRDRLLANGFNECILLFSDLPEIDIERCVRESINL
  FCWTPKSATYRQHAQPPKPSSDSSGGRSSAPYFSAECPDPPKTDLSRESIPLNDLKSEVSPRISAEDLID
  LCELTVTGHFKTPSKKTKSSKPKLLVVDIRNSEDFIRGHISGSINIPFSAAFTAEGELTQGPYTAMLQNF
  KGKVIVIVGHVAKHTAEFAAHLVKMKYPRICILDGGINKIKPTGLLTIPSPQI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163435
• ORF Size: 2679 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163435.2, NP_001156907.1
• RefSeq ORF: 2682 bp
• Locus ID: 93627
• UniProt ID: Q8TEA7
• Cytogenetics: 4q24
• Protein Families: Druggable Genome, Protein Kinase
• MW: 100.5 kDa
• Gene Summary: This gene encodes a protein that contains a protein kinase domain, a Rhodanase-like domain and the Tre-2/Bub2/Cdc16 (TBC) domain. The encoded protein is thought to play a role in actin organization, cell growth and cell proliferation by regulating the mammalian target of the rapamycin (mTOR) signaling pathway. This protein may also be involved in the transcriptional regulation of the components of the mTOR complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]