Slingshot homolog 1 (SSH1) (NM_001161330) Human Tagged ORF Clone

CAT#: RC228537

SSH1 (Myc-DDK-tagged)-Human slingshot homolog 1 (Drosophila) (SSH1), transcript variant 2

Product Images

Circular map for RC228537

• 

  Western blot validation of overexpression lysate (Cat# LY431565) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228537 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slingshot homolog 1
• Synonyms: SSH1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228537 representing NM_001161330
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGGTGACCCTGCAGCGCTCGCCCACGCCCAGCGCCGCCTCCTCCTCGGCCAGCAACAGCGAGT
  TGGAGGCTGGCAGCGAAGAAGATCGAAAATTAAACCTCAGCTTAAGTGAGAGCTTTTTCATGGTGAAAGG
  CGCAGCCCTCTTCTTACAACAGGGAAGCAGCCCTCAAGGCCAGCGGAGTCTTCAGCACCCCCACAAGCAT
  GCAGGTGATCTGCCTCAACATCTTCAGGTGATGATCAACCTTCTGCGTTGCGAAGACAGAATCAAGCTGG
  CAGTGCGCCTGGAGAGCGCCTGGGCGGACCGGGTCCGGTACATGGTGGTGGTGTACAGCAGCGGGCGCCA
  GGACACCGAGGAGAATATCTTGCTGGGAGTGGACTTTTCCAGTAAGGAAAGTAAAAGCTGCACCATTGGG
  ATGGTTCTCCGACTGTGGAGCGACACGAAAATCCACCTTGATGGAGATGGTGGGTTCAGCGTGAGCACAG
  CAGGAAGGATGCACATATTTAAGCCTGTGTCTGTCCAGGCCATGTGGTCTGCCCTGCAGGTGCTTCACAA
  GGCCTGCGAAGTGGCCCGGAGGCACAACTACTTCCCCGGGGGTGTAGCTCTCATCTGGGCTACCTACTAT
  GAGAGCTGCATCAGCTCCGAGCAGAGCTGCATCAACGAGTGGAACGCCATGCAGGACCTGGAGTCTACGC
  GGCCCGACTCCCCCGCGCTATTTGTGGACAAGCCCACTGAAGGGGAAAGGACCGAGCGCCTCATCAAAGC
  CAAGCTCCGAAGCATCATGATGAGCCAGGATCTAGAAAATGTGACTTCCAAAGAGATTCGTAATGAATTA
  GAGAAACAGATGAATTGTAACTTGAAGGAACTCAAGGAATTTATAGACAATGAGATGCTACTTATCTTGG
  GACAGATGGACAAGCCCTCCCTTATCTTCGATCATCTTTATCTCGGCTCTGAATGGAATGCATCCAATCT
  GGAGGAACTGCAGGGCTCAGGGGTTGATTACATTTTAAATGTTACCAGAGAAATCGATAATTTTTTTCCT
  GGCTTATTTGCATATCATAACATCCGAGTCTACGATGAAGAGACCACAGACCTCCTCGCCCACTGGAATG
  AAGCGTATCATTTTATAAACAAAGCGAAGAGGAACCATTCCAAGTGCCTGGTGCATTGCAAAATGGGCGT
  GAGTCGCTCGGCCTCCACAGTCATAGCCTATGCAATGAAGGAATTCGGCTGGCCTCTGGAAAAAGCATAT
  AACTATGTAAAGCAGAAGCGCAGCATCACGCGCCCCAACGCGGGCTTTATGAGGCAGCTGTCTGAGTATG
  AAGGCATCTTGGATGCAAGCAAACAGCGGCACAACAAGCTGTGGCGTCAGCAGACAGACAGCAGCCTCCA
  GCAGCCTGTGGATGACCCTGCAGGACCTGGCGACTTCTTGCCAGAGACCCCAGATGGCACCCCGGAAAGC
  CAGCTGCCCTTCTTGGATGATGCCGCCCAGCCCGGCTTAGGGCCCCCCCTCCCCTGCTGTTTCCGGCGAC
  TCTCAGACCCCCTTCTGCCTTCCCCTGAGGATGAAACTGGCAGCTTGGTCCACCTGGAGGATCCGGAGAG
  GGAGGCTCTGTTGGAGGAAGCTGCTCCACCTGCAGAGGTGCACAGGCCGGCCAGACAGCCCCAGCAAGGT
  TCCGGACTCTGTGAGAAGGATGTGAAGAAGAAACTAGAGTTTGGGAGTCCCAAAGGTCGGAGCGGCTCCT
  TGCTGCAGGTGGAGGAGACGGAAAGGGAGGAGGGCCTGGGAGCAGGGAGGTGGGGGCAGCTTCCAACCCA
  GCTCGATCAAAACCTGCTCAACTCGGAGAACCTAAACAACAACAGCAAGAGGAGCTGTCCCAACGGCATG
  GAGGTAGGCAGAGCCCGGCCTGCAGGGTGGCACACCCCATCCCTTCCATCCCACTCTAATTGGCCTACCT
  CAGCCTCTGTAGTAGGGACTACAGGCACCCGCCACCACACCCAGCTGATTTTTTTCTATTGTCTCCTCTG
  GGCCCCCAGCTCCCATCTCCAGGGACCTGAGGGTTCTTTCACAGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228537 representing NM_001161330
  Red=Cloning site Green=Tags(s)
  
  MALVTLQRSPTPSAASSSASNSELEAGSEEDRKLNLSLSESFFMVKGAALFLQQGSSPQGQRSLQHPHKH
  AGDLPQHLQVMINLLRCEDRIKLAVRLESAWADRVRYMVVVYSSGRQDTEENILLGVDFSSKESKSCTIG
  MVLRLWSDTKIHLDGDGGFSVSTAGRMHIFKPVSVQAMWSALQVLHKACEVARRHNYFPGGVALIWATYY
  ESCISSEQSCINEWNAMQDLESTRPDSPALFVDKPTEGERTERLIKAKLRSIMMSQDLENVTSKEIRNEL
  EKQMNCNLKELKEFIDNEMLLILGQMDKPSLIFDHLYLGSEWNASNLEELQGSGVDYILNVTREIDNFFP
  GLFAYHNIRVYDEETTDLLAHWNEAYHFINKAKRNHSKCLVHCKMGVSRSASTVIAYAMKEFGWPLEKAY
  NYVKQKRSITRPNAGFMRQLSEYEGILDASKQRHNKLWRQQTDSSLQQPVDDPAGPGDFLPETPDGTPES
  QLPFLDDAAQPGLGPPLPCCFRRLSDPLLPSPEDETGSLVHLEDPEREALLEEAAPPAEVHRPARQPQQG
  SGLCEKDVKKKLEFGSPKGRSGSLLQVEETEREEGLGAGRWGQLPTQLDQNLLNSENLNNNSKRSCPNGM
  EVGRARPAGWHTPSLPSHSNWPTSASVVGTTGTRHHTQLIFFYCLLWAPSSHLQGPEGSFTG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161330
• ORF Size: 2076 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161330.2
• RefSeq ORF: 2079 bp
• Locus ID: 54434
• UniProt ID: Q8WYL5
• Cytogenetics: 12q24.11
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Regulation of actin cytoskeleton
• MW: 77.2 kDa
• Gene Summary: The protein encoded by this gene belongs to the slingshot homolog (SSH) family of phosphatases, which regulate actin filament dynamics. The SSH proteins dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Cofilin is inactivated by kinases such as LIM domain kinase-1 (LIMK1), which may also be dephosphorylated and inactivated by SSH proteins. The SSH family thus appears to play a role in actin dynamics by reactivating cofilin proteins. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]