POU6F2 (NM_001166018) Human Tagged ORF Clone

CAT#: RC228521

POU6F2 (Myc-DDK-tagged)-Human POU class 6 homeobox 2 (POU6F2), transcript variant 2

Product Images

Circular map for RC228521

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  Western blot validation of overexpression lysate (Cat# LY431549) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228521 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified POU6F2 protein (Cat# TP328521). The protein was produced from HEK293T cells transfected with POU6F2 cDNA clone (Cat# RC228521) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POU6F2
• Synonyms: RPF-1; WT5; WTSL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228521 representing NM_001166018
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGCTCTTCTTCAGGATCCAATGATAGCTGGACAAGTCAGTAAGCCCTTGCTGTCAGTGCGGAGTG
  AAATGAATGCGGAGTTGAGAGGTGAGGACAAGGCTGCTACTTCAGACAGCGAGCTGAATGAGCCCCTGCT
  TGCGCCTGTGGAATCAAATGACAGCGAGGACACTCCCAGCAAGCTCTTCGGGGCTAGAGGAAACCCAGCA
  TTATCAGACCCAGGCACTCCTGACCAACACCAGGCCAGTCAGACCCACCCCCCATTTCCAGTTGGGCCAC
  AGCCACTTCTGACGGCACAGCAGTTAGCTTCTGCTGTGGCCGGCGTGATGCCGGGAGGCCCCCCAGCCCT
  CAACCAGCCAATCCTCATTCCCTTCAACATGGCGGGACAGCTAGGAGGCCAGCAAGGACTGGTTCTCACA
  CTGCCAACAGCGAATCTCACCAACATCCAAGGGCTGGTGGCAGCAGCTGCAGCCGGAGGCATTATGACTC
  TGCCACTGCAAAATCTACAAGCTACCTCATCCCTGAACTCCCAGCTCCAGCAGCTCCAGCTCCAGCTCCA
  GCAGCAGCAGCAGCAGCAGCAGCAGCAGCCTCCCCCGTCAACCAACCAGCACCCGCAACCAGCCCCACAG
  GCGCCCTCGCAGTCCCAGCAGCAGCCGCTGCAGCCCACCCCACCCCAGCAGCCACCACCCGCCTCTCAGC
  AGCCGCCAGCTCCTACATCTCAGCTGCAACAGGCGCCTCAGCCCCAGCAGCACCAACCCCACTCCCACTC
  CCAGAACCAGAACCAACCATCTCCAACCCAGCAGAGCTCCAGCCCCCCGCAGAAACCTAGTCAGTCTCCA
  GGACATGGCCTGCCTTCACCGCTCACGCCACCCAATCCTCTACAGCTGGTTAATAATCCACTAGCAAGTC
  AGGCTGCAGCGGCTGCAGCAGCCATGAGCTCCATAGCAAGCTCACAGGCCTTTGGCAATGCCCTCTCCAG
  TCTTCAGGGGGTCACAGGTCAACTAGTTACTAATGCACAAGGACAGATTATCGGGACCATTCCACTGATG
  CCTAATCCAGGGCCATCGAGCCAAGCAGCAAGCGGCACTCAGGGCTTGCAAGTGCAGCCAATCACCCCCC
  AGCTCCTCACAAACGCCCAGGGCCAGATCATCGCCACAGTCATTGGGAACCAGATCCTGCCCGTGATCAA
  CACCCAGGGCATCACGCTGTCACCCATCAAGCCCGGCCAGCAGCTCCACCAACCCTCCCAGACGTCAGTG
  GGTCAAGCAGCCTCCCAAGGCAACCTTCTGCACCTGGCTCACAGCCAAGCATCCATGTCTCAAAGTCCCG
  TCCGGCAGGCTTCCTCTTCTTCCTCCTCATCCTCCTCTTCTTCAGCTTTGAGCGTGGGCCAGTTAGTCAG
  CAATCCTCAAACGGCAGCGGGTGAGGTGGATGGGGTTAATCTGGAGGAGATCCGAGAATTTGCCAAAGCT
  TTTAAAATCCGGCGCCTGTCCCTTGGCCTGACCCAGACTCAGGTGGGACAGGCTCTCAGTGCTACAGAGG
  GCCCCGCGTACAGCCAGTCGGCCATCTGCAGGTTTGAAAAGCTGGACATCACCCCTAAAAGTGCCCAGAA
  GATCAAGCCGGTGCTTGAGCGGTGGATGGCTGAGGCTGAGGCCCGCCATCGAGCAGGTATGCAGAACCTG
  ACCGAGTTTATCGGGAGTGAACCATCCAAAAAGCGCAAGCGGCGCACCTCCTTCACACCCCAGGCCCTTG
  AGATCCTCAATGCCCACTTTGAGAAGAACACACACCCTTCTGGGCAGGAAATGACCGAAATTGCTGAGAA
  GCTGAACTATGACCGAGAAGTAGTTAGAGTTTGGTTCTGCAATAAGAGGCAAGCCCTGAAGAACACAATT
  AAACGCTTAAAACAGCACGAGCCGGCCACGGCAGTCCCTTTGGAGCCCTTAACAGACTCTCTGGAAGAAA
  ACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228521 representing NM_001166018
  Red=Cloning site Green=Tags(s)
  
  MSALLQDPMIAGQVSKPLLSVRSEMNAELRGEDKAATSDSELNEPLLAPVESNDSEDTPSKLFGARGNPA
  LSDPGTPDQHQASQTHPPFPVGPQPLLTAQQLASAVAGVMPGGPPALNQPILIPFNMAGQLGGQQGLVLT
  LPTANLTNIQGLVAAAAAGGIMTLPLQNLQATSSLNSQLQQLQLQLQQQQQQQQQQPPPSTNQHPQPAPQ
  APSQSQQQPLQPTPPQQPPPASQQPPAPTSQLQQAPQPQQHQPHSHSQNQNQPSPTQQSSSPPQKPSQSP
  GHGLPSPLTPPNPLQLVNNPLASQAAAAAAAMSSIASSQAFGNALSSLQGVTGQLVTNAQGQIIGTIPLM
  PNPGPSSQAASGTQGLQVQPITPQLLTNAQGQIIATVIGNQILPVINTQGITLSPIKPGQQLHQPSQTSV
  GQAASQGNLLHLAHSQASMSQSPVRQASSSSSSSSSSSALSVGQLVSNPQTAAGEVDGVNLEEIREFAKA
  FKIRRLSLGLTQTQVGQALSATEGPAYSQSAICRFEKLDITPKSAQKIKPVLERWMAEAEARHRAGMQNL
  TEFIGSEPSKKRKRRTSFTPQALEILNAHFEKNTHPSGQEMTEIAEKLNYDREVVRVWFCNKRQALKNTI
  KRLKQHEPATAVPLEPLTDSLEENS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166018
• ORF Size: 1965 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166018.2
• RefSeq ORF: 1968 bp
• Locus ID: 11281
• UniProt ID: P78424
• Cytogenetics: 7p14.1
• Protein Families: Transcription Factors
• MW: 69.1 kDa
• Gene Summary: This gene encodes a member of the POU protein family characterized by the presence of a bipartite DNA binding domain, consisting of a POU-specific domain and a homeodomain, separated by a variable polylinker. The DNA binding domain may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner. The POU family members are transcriptional regulators, many of which are known to control cell type-specific differentiation pathways. This gene is a tumor suppressor involved in Wilms tumor (WT) predisposition. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Oct 2009]