SRP54 (NM_001146282) Human Tagged ORF Clone

CAT#: RC228370

SRP54 (Myc-DDK-tagged)-Human signal recognition particle 54kDa (SRP54), transcript variant 2

Product Images

Circular map for RC228370

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  Western blot validation of overexpression lysate (Cat# LY431398) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228370 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SRP54
• Synonyms: SCN8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228370 representing NM_001146282
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACCATTATCAATGAAGAGGTCTGCTATTGATCTTGAAGAGATGGCATCTGGTCTTAACAAAAGAA
  AAATGATTCAGCATGCTGTATTTAAAGAACTTGTGAAGCTTGTAGACCCTGGAGTTAAGGCATGGACACC
  CACTAAAGGAAAACAAAATGTGATTATGTTTGTTGGATTGCAAGGGAGTGGTAAAACAACAACATGTTCA
  AAGCTAGCATATTATTACCAGAGGAAAGGTTGGAAGACCTGTTTAATATGTGCAGACACATTCAGAGCAG
  GGGCTTTTGACCAACTAAAACAGAATGCTACCAAAGCAAGAATTCCATTTTATGGAAGCTATACAGAAAT
  GGATCCTGTCATCATTGCTTCTGAAGGAGTAGAGAAATTTAAAAATGAAAATTTTGAAATTATTATTGTT
  GATACAAGTGGCCGCCACAAACAAGAAGACTCTTTGTTTGAAGAAATGCTTCAAGTTGCTAATGCTATAC
  AACCTGATAACATTGTTTATGTGATGGATGCCTCCATTGGGCAGGCTTGTGAAGCCCAGGCTAAGGCTTT
  TAAAGATAAAGTAGATGTAGCCTCAGTAATAGTGACAAAACTTGATGGCCATGCAAAAGGAGGTGGTGCA
  CTCAGTGCAGTCGCTGCCACAAAAAGTCCGATTATTTTCATTGGTACAGGGGAACATATAGATGACTTTG
  AACCTTTCAAAACACAGCCTTTTATTAGCAAACTTCTTGGTATGGGCGACATTGAAGGACTGATAGATAA
  AGTCAACGAGTTGAAGTTGGATGACAATGAAGCACTTATAGAGAAGTTGAAACATGGTCAGTTTACGTTG
  CGAGACATGTATGAGCAATTTCAAAATATCATGAAAATGGGCCCCTTCAGTCAGATCTTGGGGATGATCC
  CTGGTTTTGGGACAGATTTTATGAGCAAAGGAAATGAACAGGAGTCAATGGCAAGGCTAAAGAAATTAAT
  GACAATAATGGATAGTATGAATGATCAAGAACTAGACAGTACGGATGGTGCCAAAGTTTTTAGTAAACAA
  CCAGGAAGAATCCAAAGAGTAGCAAGAGGATCGGGTGTATCAACAAGAGATGTTCAAGAACTTTTGACAC
  AATATACCAAGTTTGCACAGATGGTAAAAAAGATGGGAGGTATCAAAGGACTTTTCAAAGGTGGCGACAT
  GTCTAAGAATGTGAGCCAGTCACAGATGGCAAAATTGAACCAACAAATGGCCAAAATGATGGATCCTAGG
  GTTCTTCATCACATGGGTGGTATGGCAGGACTTCAGTCAATGATGAGGCAGTTTCAACAGGGTGCTGCTG
  GCAACATGAAAGGCATGATGGGATTCAATAATATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228370 representing NM_001146282
  Red=Cloning site Green=Tags(s)
  
  MPPLSMKRSAIDLEEMASGLNKRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCS
  KLAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIV
  DTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGA
  LSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTL
  RDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQ
  PGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPR
  VLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146282
• ORF Size: 1365 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146282.1, NP_001139754.1
• RefSeq ORF: 1368 bp
• Locus ID: 6729
• UniProt ID: P61011
• Cytogenetics: 14q13.2
• Protein Pathways: Protein export
• MW: 50.2 kDa
• Gene Summary: Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).[UniProtKB/Swiss-Prot Function]