JMJD4 (NM_001161465) Human Tagged ORF Clone

CAT#: RC228358

JMJD4 (Myc-DDK-tagged)-Human jumonji domain containing 4 (JMJD4), transcript variant 2

Product Images

Circular map for RC228358

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  Western blot validation of overexpression lysate (Cat# LY431386) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228358 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: JMJD4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228358 representing NM_001161465
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTGCAGGCCCGGAGCCCCAGGCGCTGGCGGGGCAGAAACGCGGCGCCCTGCGTCTTCTGGTTCCGA
  GGCTGGTCCTCACCGTTTCCGCTCCGGCGGAAGTGAGGAGGAGGGTCCTTCGACCCGTGCTGAGCTGGAT
  GGACCGCGAGACGCGCGCCCTCGCCGACAGCCACTTCCGAGGCCTGGGGGTCGATGTCCCCGGCGTCGGC
  CAGGCTCCGGGCCGGGTAGCCTTCGTCTCGGAGCCGGGCGCCTTCTCCTACGCCGACTTTGTGCGGGGCT
  TCTTGCTGCCCAACCTGCCCTGCGTGTTTTCCAGCGCCTTCACGCAGGGCTGGGGCAGCCGGCGGCGCTG
  GGTGACGCCCGCGGGGAGGCCCGACTTCGACCACCTGCTACGGACCTACGGAGACGTGGTTGTACCAGTT
  GCAAACTGTGGGGTCCAGGAATACAACTCGAACCCCAAAGAGCACATGACTCTCAGAGACTACATCACCT
  ACTGGAAAGAGTACATACAGGCGGGCTACTCCTCTCCCAGGGGCTGTCTCTACCTCAAAGACTGGCACTT
  GTGCAGGGACTTTCCGGTGGAGGACGTTTTCACCCTGCCTGTGTACTTCTCGTCCGACTGGCTGAATGAG
  TTCTGGGATGCACTGGATGTGGATGACTACCGCTTTGTCTACGCGGGGCCTGCGGGCAGCTGGTCCCCGT
  TCCATGCTGACATCTTCCGCTCCTTCAGCTGGTCTGTCAATGTCTGTGGGAGGAAGAAGTGGCTCCTCTT
  CCCCCCAGGGCAGGAAGAGGCCCTGCGGGACCGCCACGGCAACCTGCCCTACGACGTGACCTCCCCAGCA
  CTCTGCGACACACACCTGCACCCACGGAACCAGCTTGCTGGCCCACCCTTGGAGATCACGCAGGAAGCGG
  GCGAGATGGTGTTTGTGCCCAGTGGCTGGCACCACCAGGTGCACAACCTGGATGACACCATCTCCATCAA
  CCACAACTGGGTCAATGGCTTCAACCTGGCCAACATGTGGCGCTTCTTGCAGCAGGAGCTATGCGCCGTG
  CAGGAGGAGGTCATCATGAGGTCCTGCTCGGGCATCAACTTTGAAGAGTTTTACCACTTCCTCAAGGTCA
  TCGCTGAGAAGAGGCTCCTGGTCCTGAGGGAGGCAGCCGCTGAGGACGGTGCTGGGTTGGGTTTCGAACA
  GGCAGCCTTTGATGTTGGGCGCATCACAGAGGTGCTGGCCTCCTTGGTTGCGCACCCCGACTTCCAGAGA
  GTGGACACCAGCGCGTTCTCACCACAGCCCAAAGAGCTGCTGCAGCAGCTGAGAGAGGCTGTTGATGCTG
  CTGCGGCCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228358 representing NM_001161465
  Red=Cloning site Green=Tags(s)
  
  MRAGPEPQALAGQKRGALRLLVPRLVLTVSAPAEVRRRVLRPVLSWMDRETRALADSHFRGLGVDVPGVG
  QAPGRVAFVSEPGAFSYADFVRGFLLPNLPCVFSSAFTQGWGSRRRWVTPAGRPDFDHLLRTYGDVVVPV
  ANCGVQEYNSNPKEHMTLRDYITYWKEYIQAGYSSPRGCLYLKDWHLCRDFPVEDVFTLPVYFSSDWLNE
  FWDALDVDDYRFVYAGPAGSWSPFHADIFRSFSWSVNVCGRKKWLLFPPGQEEALRDRHGNLPYDVTSPA
  LCDTHLHPRNQLAGPPLEITQEAGEMVFVPSGWHHQVHNLDDTISINHNWVNGFNLANMWRFLQQELCAV
  QEEVIMRSCSGINFEEFYHFLKVIAEKRLLVLREAAAEDGAGLGFEQAAFDVGRITEVLASLVAHPDFQR
  VDTSAFSPQPKELLQQLREAVDAAAAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161465
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161465.1, NP_001154937.1
• RefSeq ORF: 1206 bp
• Locus ID: 65094
• UniProt ID: Q9H9V9
• Cytogenetics: 1q42.13
• MW: 50.3 kDa
• Gene Summary: Catalyzes the 2-oxoglutarate and iron-dependent C4-lysyl hydroxylation of ETF1 at 'Lys-63' thereby promoting the translational termination efficiency of ETF1.[UniProtKB/Swiss-Prot Function]