PIP4K2C (NM_001146259) Human Tagged ORF Clone

CAT#: RC228323

PIP4K2C (Myc-DDK-tagged)-Human phosphatidylinositol-5-phosphate 4-kinase, type II, gamma (PIP4K2C), transcript variant 3

Product Images

Circular map for RC228323

• 

  Western blot validation of overexpression lysate (Cat# LY431351) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228323 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PIP4K2C
• Synonyms: PIP5K2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228323 representing NM_001146259
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCTCCTCGGTCCCACCAGCCACGGTATCGGCGGCGACAGCAGGCCCCGGCCCAGGTTTCGGCT
  TCGCCTCCAAGACCAAGAAGAAGCATTTCGTGCAGCAGAAGGTGAAGGTGTTCCGGGCGGCCGACCCGCT
  GGTGGGTGTGTTCCTGTGGGGCGTAGCCCACTCGATCAATGAGCTCAGCCAGGTGCCTCCCCCGGTGATG
  CTGCTGCCAGATGACTTTAAGGCCAGCTCCAAGATCAAGGAGTATTGTCCCCAGGTCTTCAGGAACCTCC
  GTGATCGATTTGGCATTGATGACCAAGATTACTTGGTGTCCCTTACCCGAAACCCCCCCAGCGAAAGTGA
  AGGCAGTGATGGTCGCTTCCTTATCTCCTACGATCGGACTCTGGTCATCAAAGAAGTATCCAGTGAGGAC
  ATTGCTGACATGCATAGCAACCTCTCCAACTATCACCAGTACATTGTGAAGTGCCATGGCAACACGCTTC
  TGCCCCAGTTCCTGGGGATGTACCGAGTCAGTGTGGACAACGAAGACAGCTACATGCTTGTGATGCGCAA
  TATGTTTAGCCACCGTCTTCCTGTGCACAGGAAGTATGACCTCAAGGGTTCCCTAGTGTCCCGGGAAGCC
  AGCGATAAGGAAAAGGTTAAAGAATTGCCCACCCTTAAGGATATGGACTTTCTCAACAAGAACCAGAAAG
  TATATATTGGTGAAGAGGAGAAGAAAATATTTCTGGAGAAGCTGAAGAGAGATGTGGAGTTTCTAGTGCA
  GCTGAAGATCATGGACTACAGCCTTCTGCTAGGCATCCACGACATCATTCGGGGCTCTGAACCAGAGGAG
  GAAGCGCCCGTGCGGGAGGATGAGTCAGAGGTGGATGGGGACTGCAGCCTGACTGGACCTCCTGCTCTGG
  TGGGCTCCTATGGCACCTCCCCAGAGGGTATCGGAGGCTACATCCATTCCCATCGGCCCCTGGGCCCAGG
  AGAGTTTGAGTCCTTCATTGATGTCTATGCCATCCGGAGTGCTGAAGGAGCCCCCCAGAAGGAGGTCTAC
  TTCATGGGCCTCATTGATATCCTTACACAGTATGATGCCAAGAAGAAAGCAGCTCATGCAGCCAAAACTG
  TCAAGCATGGGGCTGGGGCAGAGATCTCTACTGTCCATCCGGAGCAGTATGCTAAGCGATTCCTGGATTT
  TATTACCAACATCTTTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228323 representing NM_001146259
  Red=Cloning site Green=Tags(s)
  
  MASSSVPPATVSAATAGPGPGFGFASKTKKKHFVQQKVKVFRAADPLVGVFLWGVAHSINELSQVPPPVM
  LLPDDFKASSKIKEYCPQVFRNLRDRFGIDDQDYLVSLTRNPPSESEGSDGRFLISYDRTLVIKEVSSED
  IADMHSNLSNYHQYIVKCHGNTLLPQFLGMYRVSVDNEDSYMLVMRNMFSHRLPVHRKYDLKGSLVSREA
  SDKEKVKELPTLKDMDFLNKNQKVYIGEEEKKIFLEKLKRDVEFLVQLKIMDYSLLLGIHDIIRGSEPEE
  EAPVREDESEVDGDCSLTGPPALVGSYGTSPEGIGGYIHSHRPLGPGEFESFIDVYAIRSAEGAPQKEVY
  FMGLIDILTQYDAKKKAAHAAKTVKHGAGAEISTVHPEQYAKRFLDFITNIFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146259
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146259.1, NP_001139731.1
• RefSeq ORF: 1212 bp
• Locus ID: 79837
• UniProt ID: Q8TBX8
• Cytogenetics: 12q13.3
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Phosphatidylinositol signaling system, Regulation of actin cytoskeleton
• MW: 44.9 kDa
• Gene Summary: May play an important role in the production of Phosphatidylinositol bisphosphate (PIP2), in the endoplasmic reticulum.[UniProtKB/Swiss-Prot Function]