EBNA1 binding protein 2 (EBNA1BP2) (NM_001159936) Human Tagged ORF Clone

CAT#: RC228292

EBNA1BP2 (Myc-DDK-tagged)-Human EBNA1 binding protein 2 (EBNA1BP2), transcript variant 1

Product Images

Circular map for RC228292

• 

  Western blot validation of overexpression lysate (Cat# LY431320) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228292 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EBNA1 binding protein 2
• Synonyms: EBP2; NOBP; P40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228292 representing NM_001159936
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATCCAGAGGCGCTACCTGTAGGGATCCTAAGCAACCCGGACACTTTCAAACGCCGTAGCGGTTCCT
  ATAGCAACGACAAACCGGAAGTATGGTTTGCCGCCGGAAGCGGAAGTCCCAATCAAAAGTTGAGCAGTAG
  CTGTGTGGGACGTGCGTGCGGCGAGATGGACACTCCCCCGCTCTCGGATTCGGAGTCGGAATCCGATGAA
  TCCCTTGTCACAGACAGAGAGTTGCAGGATGCGTTTTCCCGAGGGCTTCTGAAGCCAGGCCTCAATGTCG
  TGCTAGAGGGGCCGAAGAAGGCCGTGAACGACGTGAATGGCCTGAAGCAATGTTTGGCAGAATTCAAGCG
  GGATCTGGAATGGGTTGAAAGGCTCGATGTGACACTGGGTCCGGTACCGGAGATCGGTGGATCTGAGGCG
  CCAGCACCTCAGAACAAGGACCAGAAAGCTGTTGATCCAGAAGACGACTTCCAGCGAGAGATGAGTTTCT
  ATCGCCAAGCCCAGGCCGCAGTGCTTGCAGTCTTACCCCGCCTCCATCAGCTCAAAGTCCCTACGAAGCG
  ACCCACTGATTATTTTGCGGAAATGGCCAAATCTGATCTGCAGATGCAGAAGATTCGACAGAAGCTGCAG
  ACTAAACAGGCTGCCATGGAGAGGTCTGAAAAAGCTAAGCAACTGCGAGCACTTAGGAAATACGGGAAGA
  AGGTGCAAACGGAGGTTCTTCAGAAGAGGCAGCAGGAGAAAGCCCATATGATGAATGCTATTAAGAAATA
  TCAGAAAGGCTTCTCTGATAAACTGGATTTCCTTGAGGGAGATCAGAAACCTCTGGCACAGCGCAAGAAG
  GCAGGAGCCAAAGGCCAGCAGATGAGGAAGGGGCCCAGTGCTAAACGACGGTATAAAAACCAGAAGTTTG
  GTTTTGGTGGAAAGAAGAAAGGCTCAAAGTGGAACACTCGGGAGAGCTATGATGATGTATCTAGCTTCCG
  GGCCAAGACAGCTCATGGCAGAGGCCTCAAGAGGCCTGGCAAGAAAGGGTCAAATAAGAGACCTGGAAAA
  CGAACAAGAGAGAAGATGAAGAACAGAACACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228292 representing NM_001159936
  Red=Cloning site Green=Tags(s)
  
  MYPEALPVGILSNPDTFKRRSGSYSNDKPEVWFAAGSGSPNQKLSSSCVGRACGEMDTPPLSDSESESDE
  SLVTDRELQDAFSRGLLKPGLNVVLEGPKKAVNDVNGLKQCLAEFKRDLEWVERLDVTLGPVPEIGGSEA
  PAPQNKDQKAVDPEDDFQREMSFYRQAQAAVLAVLPRLHQLKVPTKRPTDYFAEMAKSDLQMQKIRQKLQ
  TKQAAMERSEKAKQLRALRKYGKKVQTEVLQKRQQEKAHMMNAIKKYQKGFSDKLDFLEGDQKPLAQRKK
  AGAKGQQMRKGPSAKRRYKNQKFGFGGKKKGSKWNTRESYDDVSSFRAKTAHGRGLKRPGKKGSNKRPGK
  RTREKMKNRTH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159936
• ORF Size: 1083 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159936.1, NP_001153408.1
• RefSeq ORF: 1086 bp
• Locus ID: 10969
• UniProt ID: Q99848
• Cytogenetics: 1p34.2
• MW: 40.5 kDa
• Gene Summary: Required for the processing of the 27S pre-rRNA.[UniProtKB/Swiss-Prot Function]