ZDHHC15 (NM_001146256) Human Tagged ORF Clone

CAT#: RC228259

ZDHHC15 (Myc-DDK-tagged)-Human zinc finger, DHHC-type containing 15 (ZDHHC15), transcript variant 2

Product Images

Circular map for RC228259

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  Western blot validation of overexpression lysate (Cat# LY431287) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228259 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZDHHC15
• Synonyms: DHHC15; MRX91
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228259 representing NM_001146256
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCGAGGCTGGAAGATGGCTCTGTCTGGGGGGCTGCGGTGCTGCCGCCGGGTACTGTCCTGGGTGC
  CAGTGCTCGTTATTGTCCTCGTCGTGCTCTGGTCCTACTATGCCTACGTCTTTGAACTCTGCCTGGTTAT
  TTACCTCATACTCTACCATGCCATCTTTGTGTTCTTTACCTGGACCTACTGGAAGTCTATCTTTACACTC
  CCACAGCAGCCAAACCAGAAGTTCCACTTGTCCTACACAGACAAGGAGCGCTATGAAAATGAAGAAAGAC
  CTGAGGTCCAGAAGCAGATGCTTGTTGATATGGCCAAAAAGCTACCGGTTTACACAAGAACTGGAAGTGG
  AGCTGTACGATTCTGTGACCGGTGTCATCTGATCAAGCCAGACCGCTGCCACCACTGCTCTGTCTGTGCT
  ATGTGTGTGTTAAAAATGGATCATCACTGCCCTTGGGTTAATAACTGCATTGGATTTTCCAACTACAAAT
  TCTTCCTTCAATTCTTAGCTTACTCTGTTCTCTACTGCCTGTACATTGCTACGACAGTCTTCAGCTATTT
  CATCAAATACTGGAGAGGGGAATTACCCAGTGTTCGCTCTAAGTTCCATGTCCTTTTTCTTCTCTTTGTG
  GCCTGCATGTTTTTTGTCAGCCTTGTGATTCTCTTTGGTTACCATTGTTGGCTTGTCAGCAGAAACAAAA
  CCACCTTAGAGGCCTTCTGCACTCCAGTGTTTACAAGTGGCCCAGAGAAAAATGGGTTCAACCTTGGCTT
  CATCAAGAATATCCAGCAGGTGTTTGGAGATAAGAAGAAGTTCTGGTTAATACCTATTGGTTCCAGCCCT
  GGTGATGGACACTCCTTCCCTATGAGGTCTATGAATGAGTCACAGAACCCACTGCTAGCAAATGAAGAAA
  CCTGGGAAGACAACGAGGATGACAACCAAGATTATCCAGAAGGCTCATCATCTCTTGCTGTGGAAACGGA
  AACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228259 representing NM_001146256
  Red=Cloning site Green=Tags(s)
  
  MRRGWKMALSGGLRCCRRVLSWVPVLVIVLVVLWSYYAYVFELCLVIYLILYHAIFVFFTWTYWKSIFTL
  PQQPNQKFHLSYTDKERYENEERPEVQKQMLVDMAKKLPVYTRTGSGAVRFCDRCHLIKPDRCHHCSVCA
  MCVLKMDHHCPWVNNCIGFSNYKFFLQFLAYSVLYCLYIATTVFSYFIKYWRGELPSVRSKFHVLFLLFV
  ACMFFVSLVILFGYHCWLVSRNKTTLEAFCTPVFTSGPEKNGFNLGFIKNIQQVFGDKKKFWLIPIGSSP
  GDGHSFPMRSMNESQNPLLANEETWEDNEDDNQDYPEGSSSLAVETET
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146256
• ORF Size: 984 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146256.1, NP_001139728.1
• RefSeq ORF: 987 bp
• Locus ID: 158866
• UniProt ID: Q96MV8
• Cytogenetics: Xq13.3
• Protein Families: Transmembrane
• MW: 38.2 kDa
• Gene Summary: The protein encoded by this gene belongs to the DHHC palmitoyltransferase family. Mutations in this gene are associated with mental retardatio X-linked type 91 (MRX91). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]