PIGX (NM_001166304) Human Tagged ORF Clone

CAT#: RC228208

PIGX (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class X (PIGX), transcript variant 1

Product Images

Circular map for RC228208

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  Coomassie blue staining of purified PIGX protein (Cat# TP328208). The protein was produced from HEK293T cells transfected with PIGX cDNA clone (Cat# RC228208) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PIGX
• Synonyms: PIG-X
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228208 representing NM_001166304
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  CTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCCTGGCGGCTCGGGTGGCGGCGGTTCGGGCGG
  CCGCCTGGCTGCTCCTCGGGGCGGCGACCGGGCTCACGCGCGGGCCCGCCGCGGCCTTCACCGCCGCGCG
  CTCTGACGCCGGCATAAGGGCCATGTGTTCTGAAATTATTTTGAGGCAAGAAGTTTTGAAAGATGGTTTC
  CACAGAGACCTTTTAATCAAAGTGAAGTTTGGGGAAAGCATTGAGGACTTGCACACCTGCCGTCTCTTAA
  TTAAACAGGACATTCCTGCAGGACTTTATGTGGATCCGTATGAGTTGGCTTCATTACGAGAGAGAAACAT
  AACAGAGGCAGTGATGGTTTCAGAAAATTTTGATATAGAGGCCCCTAACTATTTGTCCAAGGAGTCTGAA
  GTTCTCATTTATGCCAGACGAGATTCACAGTGCATTGACTGTTTTCAAGCCTTTTTGCCTGTGCACTGCC
  GCTATCATCGGCCGCACAGTGAAGATGGAGAAGCCTCGATTGTGGTCAATAACCCAGATTTGTTGATGTT
  TTGTGACCAAGCTGGCTCCAGAAGAATGATCAGATTCCGGTTTGATTCCTTTGACAAAACTATAGAGTTC
  CCGATTTTGAAATGCTGGGCTCACTCAGAAGTGGCAGCCCCTTGTGCTTTGGAGAATGAGGATATCTGCC
  AATGGAACAAGATGAAGTATAAATCAGTATATAAGAATGTGATTCTACAAGTTCCAGTGGGACTGACTGT
  ACATACCTCTCTAGTATGTTCTGTGACTCTGCTCATTACAATCCTGTGCTCTACATTGATCCTTGTAGCA
  GTTTTCAAATATGGCCATTTTTCCCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228208 representing NM_001166304
  Red=Cloning site Green=Tags(s)
  
  LDPVPRRSAAAIALAARVAAVRAAAWLLLGAATGLTRGPAAAFTAARSDAGIRAMCSEIILRQEVLKDGF
  HRDLLIKVKFGESIEDLHTCRLLIKQDIPAGLYVDPYELASLRERNITEAVMVSENFDIEAPNYLSKESE
  VLIYARRDSQCIDCFQAFLPVHCRYHRPHSEDGEASIVVNNPDLLMFCDQAGSRRMIRFRFDSFDKTIEF
  PILKCWAHSEVAAPCALENEDICQWNKMKYKSVYKNVILQVPVGLTVHTSLVCSVTLLITILCSTLILVA
  VFKYGHFSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166304
• ORF Size: 867 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq ORF: 831 bp
• Locus ID: 54965
• UniProt ID: Q8TBF5
• Cytogenetics: 3q29
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• MW: 32.27 kDa
• Gene Summary: This gene encodes a type I transmembrane protein in the endoplasmic reticulum (ER). The protein is an essential component of glycosylphosphatidylinositol-mannosyltransferase I, which transfers the first of the four mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Studies in rat indicate that the protein is translated from a non-AUG translation initiation site. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]