RASSF7 (NM_001143993) Human Tagged ORF Clone

CAT#: RC227985

RASSF7 (Myc-DDK-tagged)-Human Ras association (RalGDS/AF-6) domain family (N-terminal) member 7 (RASSF7), transcript variant 2

Product Images

Circular map for RC227985

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  Western blot validation of overexpression lysate (Cat# LY428453) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227985 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RASSF7
• Synonyms: C11orf13; CFAP88; FAP88; HRAS1; HRC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227985 representing NM_001143993
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTTGGGACTGGCGGCCATGGAGCTGAAGGTGTGGGTGGATGGCATCCAGCGTGTGGTCTGTGGGG
  TCTCAGAGCAGACCACCTGCCAGGAAGTGGTCATCGCACTAGCCCAAGCAATAGGCCAGACTGGCCGCTT
  TGTGCTTGTGCAGCGGCTTCGGGAGAAGGAGCGGCAGTTGCTGCCACAAGAGTGTCCAGTGGGCGCCCAG
  GCCACCTGCGGACAGTTTGCCAGCGATGTCCAGTTTGTCCTGAGGCGCACAGGGCCCAGCCTAGCTGGGA
  GGCCCTCCTCAGACAGCTGTCCACCCCCGGAACGCTGCCTAATTCGTGCCAGCCTCCCTGTAAAGCCACG
  GGCTGCGCTGGGCTGTGAGCCCCGCAAAACACTGACCCCCGAGCCAGCCCCCAGCCTCTCACGCCCTGGG
  CCTGCGGCCCCTGTGACACCCACACCAGGCTGCTGCACAGACCTGCGGGGCCTGGAGCTCAGGGTGCAGA
  GGAATGCTGAGGAGCTGGGCCATGAGGCCTTCTGGGAGCAAGAGCTGCGCCGGGAGCAGGCCCGGGAGCG
  AGAGGGACAGGCACGCCTGCAGGCACTAAGTGCGGCCACTGCTGAGCATGCCGCCCGGCTGCAGGCCCTG
  GACGCTCAGGCCCGTGCCCTGGAGGCTGAGCTGCAGCTGGCAGCGGAGGCCCCTGGGCCCCCCTCACCTA
  TGGCATCTGCCACTGAGCGCCTGCACCAGGACCTGGCTGTTCAGGAGCGGCAGAGTGCGGAGGTGCAGGG
  CAGCCTGGCTCTGGTGAGCCGGGCCCTGGAGGCAGCAGAGCGAGCCTTGCAGGCTCAGGCTCAGGAGCTG
  GAGGAGCTGAACCGAGAGCTCCGTCAGTGCAACCTGCAGCAGTTCATCCAGCAGACCGGGGCTGCGCTGC
  CACCGCCCCCACGGCCTGACAGGGGCCCTCCTGGCACTCAGGTCGGAGTGGTTCTGGGGGGAGGCTGGGA
  GGTGAGGACCTGGCCCAGCCCCACTCCAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227985 representing NM_001143993
  Red=Cloning site Green=Tags(s)
  
  MLLGLAAMELKVWVDGIQRVVCGVSEQTTCQEVVIALAQAIGQTGRFVLVQRLREKERQLLPQECPVGAQ
  ATCGQFASDVQFVLRRTGPSLAGRPSSDSCPPPERCLIRASLPVKPRAALGCEPRKTLTPEPAPSLSRPG
  PAAPVTPTPGCCTDLRGLELRVQRNAEELGHEAFWEQELRREQAREREGQARLQALSAATAEHAARLQAL
  DAQARALEAELQLAAEAPGPPSPMASATERLHQDLAVQERQSAEVQGSLALVSRALEAAERALQAQAQEL
  EELNRELRQCNLQQFIQQTGAALPPPPRPDRGPPGTQVGVVLGGGWEVRTWPSPTPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143993
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001143993.2
• RefSeq ORF: 1014 bp
• Locus ID: 8045
• UniProt ID: Q02833
• Cytogenetics: 11p15.5
• MW: 36.2 kDa
• Gene Summary: Negatively regulates stress-induced JNK activation and apoptosis by promoting MAP2K7 phosphorylation and inhibiting its ability to activate JNK. Following prolonged stress, anti-apoptotic effect stops because of degradation of RASSF7 protein via the ubiquitin-proteasome pathway. Required for the activation of AURKB and chromosomal congression during mitosis where it stimulates microtubule polymerization.[UniProtKB/Swiss-Prot Function]