TRIM66 (NM_014818) Human Tagged ORF Clone

CAT#: RC227892

TRIM66 (Myc-DDK-tagged)-Human tripartite motif containing 66 (TRIM66)

Product Images

Circular map for RC227892

• 

  Western blot validation of overexpression lysate (Cat# LY429439) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227892 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIM66
• Synonyms: C11orf29; TIF1D; TIF1DELTA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227892 representing NM_014818
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGAACTGCTCTGAGTGCAAGGAGAAGAGGGCAGCACATATCCTCTGCACCTACTGCAATCGCT
  GGCTGTGCAGCTCTTGCACAGAGGAACACCGACACAGCCCTGTCCCCGGGGGCCCATTCTTTCCTCGGGC
  CCAGAAGGGATCTCCAGGAGTGAATGGTGGTCCCGGAGACTTCACCTTGTATTGTCCTCTACACACACAG
  GAAGTACTCAAGCTATTCTGTGAGACATGTGATATGCTCACTTGCCATAGCTGCCTAGTGGTGGAACACA
  AAGAACACAGGTGCAGACATGTTGAAGAAGTTTTGCAAAACCAGAGGATGCTTCTGGAAGGTGTGACTAC
  ACAGGTGGCACATAAGAAATCCAGTCTACAGACATCTGCAAAGCAAATTGAGGACAGGATTTTTGAAGTG
  AAGCATCAGCATAGGAAGGTGGAAAACCAGATCAAAATGGCCAAGATGGTTCTGATGAATGAGCTGAACA
  AACAGGCCAATGGGCTAATAGAGGAATTAGAGGGGATTACTAATGAGAGAAAGCGGAAGCTGGAACAGCA
  GTTACAGAGCATCATGGTTCTCAACCGTCAGTTTGAGCATGTGCAGAATTTCATCAACTGGGCTGTCTGC
  AGCAAAACCAGTGTCCCTTTTCTTTTCAGCAAAGAGCTGATTGTGTTTCAGATGCAGCGATTGCTGGAGA
  CAAGTTGTAACACAGATCCTGGCTCCCCTTGGAGTATCAGATTCACCTGGGAGCCTAACTTCTGGACCAA
  GCAGCTAGCTTCTCTTGGCTGCATAACTACTGAAGGTGGACAAATGTCCAGGGCAGATGCTCCTGCTTAT
  GGAGGCTTACAGGGGTCATCACCCTTTTATCAAAGCCACCAGTCTCCAGTGGCTCAGCAAGAGGCTCTTA
  GCCACCCCTCACACAAGTTCCAGTCTCCAGCAGTGTGCTCCTCATCTGTGTGCTGCTCCCACTGCTCCCC
  AGTCTCGCCTTCCCTCAAAGGCCAGGTCCCCCCACCCAGCATACACCCAGCCCACAGCTTCAGGCAGCCC
  CCTGAGATGGTGCCCCAGCAGCTGGGGTCTCTGCAGTGCTCTGCCCTGCTGCCCAGGGAGAAAGAGCTGG
  CCTGCAGCCCTCATCCACCAAAGCTGCTGCAGCCCTGGCTGGAAACCCAGCCCCCCGTGGAGCAGGAGAG
  CACATCCCAGCGGCTGGGGCAGCAGCTGACTTCCCAGCCCGTGTGCATTGTCCCCCCACAGGATGTTCAG
  CAAGGAGCCCATGCCCAGCCCACCTTACAGACACCCTCTATCCAAGTCCAGTTTGGCCACCACCAGAAGC
  TGAAGCTCAGTCACTTTCAGCAGCAGCCACAGCAGCAGCTACCACCTCCACCACCACCCCTCCCCCATCC
  CCCACCTCCCCTTCCCCCTCCCCCACAGCAGCCACACCCACCTCTTCCTCCATCCCAGCATCTGGCTTCT
  AGTCAGCACGAGAGCCCTCCTGGCCCTGCCTGTTCTCAGAACATGGACATAATGCATCACAAGTTTGAGC
  TGGAGGAAATGCAGAAGGACTTGGAGCTTCTTCTCCAGGCTCAACAGCCCAGCCTGCAACTGAGTCAGAC
  CAAATCTCCTCAGCATCTTCAGCAAACCATTGTGGGGCAGATCAACTACATCGTGAGGCAGCCAGCACCT
  GTCCAGTCCCAGAGCCAGGAGGAGACCCTGCAGGCTACAGATGAGCCCCCAGCATCTCAGGGCTCAAAGC
  CGGCTCTCCCTCTTGACAAGAATACTGCTGCTGCCTTGCCCCAGGCGTCTGGGGAAGAAACCCCTCTCAG
  TGTCCCCCCAGTGGACAGCACCATCCAGCACTCCTCTCCAAATGTGGTGAGAAAGCACTCCACCTCGCTG
  AGCATCATGGGCTTTTCCAACACTCTGGAGATGGAGTTGTCATCTACCAGGTTGGAGAGGCCCCTAGAGC
  CACAGATCCAGAGTGTGAGCAACCTGACAGCTGGTGCCCCCCAGGCAGTACCAAGCCTGCTGAGTGCTCC
  CCCCAAAATGGTGTCCAGCCTGACAAGTGTTCAAAACCAGGCCATGCCCAGCCTGACAACCAGTCACCTA
  CAGACTGTGCCCAGCCTTGTGCATAGCACATTCCAGTCCATGCCCAACCTGATAAGTGACTCCCCTCAGG
  CTATGGCAAGCCTGGCAAGTGATCACCCTCAGGCTGGGCCCAGCCTAATGTCTGGTCACACCCAGGCTGT
  GCCGAGTCTGGCAACTTGTCCTCTGCAGAGCATCCCTCCAGTTTCTGACATGCAGCCAGAAACTGGGTCC
  AGCTCCAGTTCTGGCCGAACTTCAGGGAGCCTGTGTCCCAGAGATGGGGCTGATCCCTCCCTGGAGAATG
  CTCTGTGTAAGGTAAAACTGGAGGAGCCAATTAACCTCTCTGTGAAGAAACCTCCACTGGCGCCAGTGGT
  CAGCACGTCTACAGCTCTGCAGCAGTACCAGAACCCAAAAGAGTGTGAGAATTTTGAACAAGGAGCCCTA
  GAGCTGGATGCAAAAGAGAACCAGAGCATCAGAGCCTTCAATAGTGAGCATAAGATTCCCTATGTGCGAC
  TGGAGCGACTCAAGATCTGTGCTGCCTCCTCAGGAGAGATGCCTGTGTTCAAACTGAAGCCACAGAAGAA
  TGATCAGGATGGGAGCTTCCTGCTGATCATCGAGTGTGGCACTGAGTCCTCCAGCATGTCCATTAAGGTC
  AGCCAGGACAGACTGTCTGAGGCCACCCAGGCCCCAGGTCTGGAGGGAAGAAAGGTCACTGTCACTTCTT
  TGGCTGGGCAGCGGCCACCAGAAGTGGAGGGCACATCTCCTGAAGAACACAGACTCATTCCTCGAACCCC
  AGGAGCCAAGAAGGGCCCCCCAGCCCCAATAGAGAATGAGGACTTCTGTGCTGTTTGCCTCAATGGCGGA
  GAGTTACTGTGCTGTGACCGCTGCCCCAAAGTGTTCCACCTCTCCTGCCATGTGCCAGCCTTGCTCAGCT
  TCCCAGGGGGAGAGTGGGTGTGTACCTTGTGCCGCAGCCTGACCCAGCCCGAGATGGAGTACGACTGTGA
  GAATGCCTGCTATAACCAGCCTGGAATGCGGGCATCTCCTGGCCTAAGCATGTATGACCAGAAGAAGTGT
  GAGAAGCTGGTATTGTCCTTGTGCTGCAATAACCTCAGCCTGCCCTTCCATGAACCTGTCAGCCCCCTGG
  CCCGGCATTATTACCAGATTATCAAGAGGCCCATGGACCTGTCAATCATCCGGAGGAAGCTGCAAAAGAA
  GGACCCAGCTCACTATACCACCCCAGAGGAGGTGGTATCAGATGTGCGCCTCATGTTCTGGAACTGTGCT
  AAGTTCAATTATCCTGACTCCGAGGTTGCAGAGGCTGGCCGCTGCCTGGAAGTGTTCTTTGAGGGCTGGT
  TGAAGGAGATCTACCCGGAGAAACGGTTTGCCCAGCCAAGGCAGGAGGACTCAGACTCCGAGGAGGTGTC
  TAGTGAGAGTGGATGTTCCACTCCCCAGGGCTTCCCGTGGCCTCCCTACATGCAGGAGGGCATCCAACCC
  AAGAGGCGGCGACGACATATGGAGAATGAAAGAGCAAAAAGAATGTCATTTCGCCTGGCCAACAGCATCT
  CTCAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227892 representing NM_014818
  Red=Cloning site Green=Tags(s)
  
  MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQ
  EVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEV
  KHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVC
  SKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAY
  GGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQP
  PEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQ
  QGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLAS
  SQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAP
  VQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSL
  SIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHL
  QTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGS
  SSSSGRTSGSLCPRDGADPSLENALCKVKLEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGAL
  ELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKV
  SQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGG
  ELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKC
  EKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCA
  KFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQP
  KRRRRHMENERAKRMSFRLANSISQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014818
• ORF Size: 3648 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014818.2
• RefSeq ORF: 3651 bp
• Locus ID: 9866
• UniProt ID: O15016
• Cytogenetics: 11p15.4
• MW: 134.5 kDa
• Gene Summary: May function as transcription repressor; The repressive effects are mediated, at least in part, by recruitment of deacetylase activity. May play a role as negative regulator of postmeiotic genes acting through CBX3 complex formation and centromere association (By similarity).[UniProtKB/Swiss-Prot Function]