ZCCHC18 (NM_001143978) Human Tagged ORF Clone

CAT#: RC227436

ZCCHC18 (Myc-DDK-tagged)-Human zinc finger, CCHC domain containing 18 (ZCCHC18), transcript variant 1

Product Images

Circular map for RC227436

• 

  Western blot validation of overexpression lysate (Cat# LY428438) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227436 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZCCHC18
• Synonyms: PNMA7B; SIZN2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227436 representing NM_001143978
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAGCATCACTGCGTGTGTGGGTAACAGCAGGCAGCAGAATGCACCTTTGCCGCCTTGGGCCCATT
  CCATGTTGAGGTCTCTGGGGAGGAGTCTCTGTCCTTTAGTGGTCAAAATGGCAGAGAGAAACATGAAGTT
  GTTCTCAGGAAGAGTGGTGCCAGCCCAGGGGAAAGAAACCTTTGAAAACTGGCTGATCCAAGTCAATGAG
  GTCCTGCCAGATTGGAGTATGTCTGAGGAGGAAAAACTCAAGCGCTTGATGAAAACACTTAGGGGCCCTG
  CCCGGGAGGTCATGCGTTTGCTTCAGGCGGCCAACCCCAACCTAAGTGTAGCAGATTTCTTGCGGGCAAT
  GAAATTGGTGTTTGGGGAGTCTGAAAGCAGTGTGACTGCCCATGGTAAATTTTTTAACACCCTGCAGGCA
  CAAGGGGAGAAAGCCTCCCTTTATGTGATCCGTTTAGAGGTGCAGCTCCAGAATGCTATTCAGGCAGGCA
  TCCTAGCTGAGAAAGATGCAAACCAGACTCGCTTGCAACAGCTTCTTTTAGGCGCTGAGCTGAATAGGGA
  CCTGCGCTTCAGGCTTAAGCATCTTCTCAGGATGTATGCAAATAAGCAGGAGCGGCTTCCCAATTTCCTG
  GAGTTAATCAAGATGATAAGGGAGGAAGAGGATTGGGATGATGCTTTTATTAAACGGAAGCGGCCGAAAA
  GGTCTGAGCCAATAATGGAGAGGGCAGCCAGCCCTGTGGCATTTCAGGGCGCCCAGCCAATAGCAATCAG
  CAGTGCTGACTGTAACTGCAACGTGATAGAAATAGATGATACCCTTGATGACTCTGATGAGGATGTGATC
  CTGGTGGTGTCTCTGTACCCTTCACTGACACCTACAGGTGCCCCTCCCTTCAGAGGAAGAGCCAGACCTC
  TGGATCAAGTGCTGGTTATTGATTCCCCCAACAATTCTGGGGCTCAGTCTCTTTCTACCAGTGGTGGTTC
  TGGGTATAAGAATGATGGTCCTGGGAATATTCGTAGAGCCAGGAAGCGAAAATACACAACCCGCTGTTCA
  TATTGTGGGGAGGAGGGCCACTCAAAAGAAACCTGTGACAATGAGAGCAACAAGGCCCAGGTTTTTGAGA
  ATCTGATCATCACCCTGCAGGAGCTGACACATACAGAGGAGAGGTCAAAAGAGGTCCCTGGAGAACACAG
  TGATGCTTCTGAGCCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227436 representing NM_001143978
  Red=Cloning site Green=Tags(s)
  
  MASITACVGNSRQQNAPLPPWAHSMLRSLGRSLCPLVVKMAERNMKLFSGRVVPAQGKETFENWLIQVNE
  VLPDWSMSEEEKLKRLMKTLRGPAREVMRLLQAANPNLSVADFLRAMKLVFGESESSVTAHGKFFNTLQA
  QGEKASLYVIRLEVQLQNAIQAGILAEKDANQTRLQQLLLGAELNRDLRFRLKHLLRMYANKQERLPNFL
  ELIKMIREEEDWDDAFIKRKRPKRSEPIMERAASPVAFQGAQPIAISSADCNCNVIEIDDTLDDSDEDVI
  LVVSLYPSLTPTGAPPFRGRARPLDQVLVIDSPNNSGAQSLSTSGGSGYKNDGPGNIRRARKRKYTTRCS
  YCGEEGHSKETCDNESNKAQVFENLIITLQELTHTEERSKEVPGEHSDASEPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143978
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001143978.1, NP_001137450.1
• RefSeq ORF: 1212 bp
• Locus ID: 644353
• UniProt ID: P0CG32
• Cytogenetics: Xq22.2
• MW: 45 kDa