ELAPOR2 (NM_001142749) Human Tagged ORF Clone

CAT#: RC227277

KIAA1324L (Myc-DDK-tagged)-Human KIAA1324-like (KIAA1324L), transcript variant 1

Product Images

Circular map for RC227277

• 

  Western blot validation of overexpression lysate (Cat# LY428259) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227277 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified KIAA1324L protein (Cat# TP327277). The protein was produced from HEK293T cells transfected with KIAA1324L cDNA clone (Cat# RC227277) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ELAPOR2
• Synonyms: EIG121L; KIAA1324L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227277 representing NM_001142749
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTTCCGCGCCCGGGGGCCGGTACGGGGCAGGGGCTGGGGGCGGCCGGCGGAGGCTCCCCGCCGCG
  GGCGCTCGCCGCCCTGGAGCCCCGCCTGGATTTGCTGCTGGGCGCTCGCCGGCTGCCAGGCGGCCTGGGC
  TGGGGACCTGCCCTCCTCCTCCAGCCGCCCGCTTCCTCCTTGCCAGGAGAAAGATTATCACTTTGAATAT
  ACGGAATGTGATAGCAGTGGCTCCAGGTGGAGAGTTGCCATTCCAAATTCTGCAGTGGACTGCTCTGGCC
  TGCCTGACCCAGTGAGAGGCAAAGAATGCACTTTCTCCTGTGCTTCTGGAGAGTATCTAGAAATGAAGAA
  CCAGGTATGCAGTAAGTGTGGTGAAGGCACCTATTCCTTGGGCAGTGGCATCAAATTTGATGAATGGGAT
  GAATTGCCGGCAGGATTTTCTAACATCGCAACATTCATGGACACTGTGGTGGGCCCTTCTGACAGCAGGC
  CAGACGGCTGTAACAACTCTTCTTGGATCCCTCGTGGAAACTACATAGAATCTAATCGTGATGACTGCAC
  GGTGTCTTTGATCTATGCTGTGCACCTTAAGAAGTCAGGCTATGTCTTCTTTGAGTACCAGTATGTCGAC
  AACAACATCTTCTTTGAGTTCTTTATTCAAAATGATCAGTGCCAGGAGATGGACACCACCACTGACAAGT
  GGGTAAAACTTACAGACAATGGAGAATGGGGCTCTCATTCTGTAATGCTGAAATCAGGCACAAACATACT
  CTACTGGAGAACTACAGGCATCCTTATGGGTTCTAAGGCGGTCAAGCCTGTGCTGGTAAAAAATATCACA
  ATTGAAGGGGTGGCGTACACATCAGAATGTTTTCCTTGCAAGCCAGGCACATTCAGCAACAAACCAGGTT
  CATTCAACTGCCAGGTGTGTCCCAGAAACACCTATTCTGAGAAAGGAGCCAAAGAATGTATAAGGTGTAA
  AGACGACTCTCAATTTTCAGAGGAAGGATCCAGTGAGTGTACAGAGCGCCCTCCCTGTACCACAAAAGAC
  TATTTCCAGATCCATACTCCATGTGATGAAGAAGGAAAGACACAGATAATGTACAAGTGGATAGAGCCCA
  AAATCTGCCGGGAGGATCTCACAGATGCTATTAGATTGCCCCCTTCTGGAGAGAAGAAGGATTGTCCGCC
  TTGCAACCCTGGATTTTATAACAATGGATCATCTTCTTGCCATCCCTGTCCTCCTGGAACATTTTCAGAT
  GGAACCAAAGAATGTAGACCATGTCCAGCAGGAACGGAGCCTGCACTTGGCTTTGAATATAAATGGTGGA
  ATGTCCTTCCTGGCAACATGAAAACTTCCTGCTTCAATGTTGGGAATTCAAAGTGCGATGGAATGAATGG
  TTGGGAGGTGGCTGGAGATCATATCCAGAGTGGGGCTGGAGGTTCTGACAATGATTACCTGATCTTAAAC
  TTGCATATCCCAGGATTTAAACCACCAACATCTATGACTGGAGCCACGGGTTCTGAACTAGGAAGAATAA
  CATTTGTCTTTGAGACCCTCTGTTCAGCTGACTGTGTTTTGTACTTCATGGTGGATATTAATAGAAAAAG
  TACAAATGTGGTAGAATCGTGGGGTGGAACCAAAGAAAAACAAGCTTACACCCATATCATCTTCAAGAAT
  GCAACTTTTACATTTACATGGGCATTCCAGAGAACTAATCAGGGTCAAGATAATAGACGGTTCATCAATG
  ACATGGTGAAGATTTATTCTATCACAGCCACTAATGCAGTTGATGGGGTGGCGTCCTCATGCCGTGCCTG
  TGCCCTCGGTTCTGAACAGTCGGGTTCATCGTGTGTCCCCTGCCCTCCAGGCCACTACATTGAGAAAGAA
  ACCAACCAGTGCAAGGAATGTCCACCTGACACCTACCTGTCCATACATCAGGTCTATGGCAAAGAGGCTT
  GTATTCCATGCGGGCCTGGGAGTAAAAACAATCAGGACCATTCGGTTTGCTATAGTGACTGCTTTTTCTA
  CCATGAAAAAGAAAATCAGAGTTTGCACTATGACTTTAGCAACCTCAGCAGTGTGGGCTCATTAATGAAT
  GGCCCCAGCTTCACCTCCAAAGGAACAAAATACTTCCATTTCTTCAATATCAGTTTATGTGGGCATGAGG
  GGAAGAAGATGGCTCTCTGTACCAACAATATAACAGACTTTACAGTAAAAGAAATAGTGGCAGGGTCAGA
  TGATTACACAAATTTGGTAGGGGCATTTGTATGCCAGTCAACAATTATTCCTTCTGAAAGTAAGGGTTTC
  CGAGCAGCCTTATCATCACAATCCATCATTCTGGCAGATACATTCATAGGAGTCACAGTTGAAACCACAT
  TGAAAAATATTAATATAAAAGAAGATATGTTCCCAGTTCCAACAAGCCAAATACCAGATGTGCATTTCTT
  TTATAAGTCTTCTACAGCAACAACATCTTGTATTAATGGCCGATCAACTGCTGTGAAAATGAGGTGTAAT
  CCTACTAAATCTGGAGCAGGAGTGATTTCAGTCCCCAGCAAGTGCCCAGCAGGTACCTGTGATGGGTGTA
  CGTTCTATTTCCTGTGGGAGAGTGCTGAAGCTTGCCCTCTGTGTACGGAGCATGACTTCCATGAGATTGA
  GGGAGCCTGCAAGAGAGGATTTCAGGAAACCTTGTATGTGTGGAATGAACCTAAATGGTGCATTAAAGGA
  ATTTCTTTGCCTGAGAAAAAGTTGGCAACCTGTGAAACGGTTGACTTTTGGCTGAAGGTGGGAGCCGGTG
  TGGGAGCTTTTACTGCCGTTTTGCTGGTGGCTCTGACCTGCTACTTCTGGAAAAAGAATCAAAAACTGGA
  ATACAAATATTCCAAGTTAGTAATGACGACTAACTCAAAAGAGTGTGAACTCCCGGCTGCAGACAGTTGT
  GCTATCATGGAAGGAGAAGATAATGAAGAGGAAGTTGTATATTCCAATAAACAGTCACTACTAGGAAAAC
  TCAAATCTTTGGCAACCAAGGAAAAAGAAGACCATTTTGAATCTGTTCAACTGAAAACCTCAAGATCCCC
  AAATATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227277 representing NM_001142749
  Red=Cloning site Green=Tags(s)
  
  MLFRARGPVRGRGWGRPAEAPRRGRSPPWSPAWICCWALAGCQAAWAGDLPSSSSRPLPPCQEKDYHFEY
  TECDSSGSRWRVAIPNSAVDCSGLPDPVRGKECTFSCASGEYLEMKNQVCSKCGEGTYSLGSGIKFDEWD
  ELPAGFSNIATFMDTVVGPSDSRPDGCNNSSWIPRGNYIESNRDDCTVSLIYAVHLKKSGYVFFEYQYVD
  NNIFFEFFIQNDQCQEMDTTTDKWVKLTDNGEWGSHSVMLKSGTNILYWRTTGILMGSKAVKPVLVKNIT
  IEGVAYTSECFPCKPGTFSNKPGSFNCQVCPRNTYSEKGAKECIRCKDDSQFSEEGSSECTERPPCTTKD
  YFQIHTPCDEEGKTQIMYKWIEPKICREDLTDAIRLPPSGEKKDCPPCNPGFYNNGSSSCHPCPPGTFSD
  GTKECRPCPAGTEPALGFEYKWWNVLPGNMKTSCFNVGNSKCDGMNGWEVAGDHIQSGAGGSDNDYLILN
  LHIPGFKPPTSMTGATGSELGRITFVFETLCSADCVLYFMVDINRKSTNVVESWGGTKEKQAYTHIIFKN
  ATFTFTWAFQRTNQGQDNRRFINDMVKIYSITATNAVDGVASSCRACALGSEQSGSSCVPCPPGHYIEKE
  TNQCKECPPDTYLSIHQVYGKEACIPCGPGSKNNQDHSVCYSDCFFYHEKENQSLHYDFSNLSSVGSLMN
  GPSFTSKGTKYFHFFNISLCGHEGKKMALCTNNITDFTVKEIVAGSDDYTNLVGAFVCQSTIIPSESKGF
  RAALSSQSIILADTFIGVTVETTLKNINIKEDMFPVPTSQIPDVHFFYKSSTATTSCINGRSTAVKMRCN
  PTKSGAGVISVPSKCPAGTCDGCTFYFLWESAEACPLCTEHDFHEIEGACKRGFQETLYVWNEPKWCIKG
  ISLPEKKLATCETVDFWLKVGAGVGAFTAVLLVALTCYFWKKNQKLEYKYSKLVMTTNSKECELPAADSC
  AIMEGEDNEEEVVYSNKQSLLGKLKSLATKEKEDHFESVQLKTSRSPNI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142749
• ORF Size: 3087 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142749.3
• RefSeq ORF: 3090 bp
• Locus ID: 222223
• UniProt ID: A8MWY0
• Cytogenetics: 7q21.12
• Protein Families: Transmembrane
• MW: 113.7 kDa
• Gene Summary: Functions as a regulator of the BMP signaling pathway and may be involved in epidermal differentiation.[UniProtKB/Swiss-Prot Function]