MUC7 (NM_001145006) Human Tagged ORF Clone

CAT#: RC227257

MUC7 (Myc-DDK-tagged)-Human mucin 7, secreted (MUC7), transcript variant 1

Product Images

Circular map for RC227257

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MUC7
• Synonyms: MG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227257 representing NM_001145006
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAACTCTGCCGCTGTTTGTGTGCATCTGTGCACTGAGTGCTTGCTTCTCGTTCAGTGAAGGTCGAG
  AAAGGGATCATGAACTACGTCACAGAAGGCATCATCACCAATCACCCAAATCTCACTTTGAATTACCACA
  TTATCCTGGACTGCTAGCTCACCAGAAGCCGTTCATTAGAAAGTCCTATAAATGTCTGCACAAACGCTGT
  AGGCCTAAGCTTCCACCTTCACCTAATAACCCCCCCAAATTCCCAAATCCTCACCAGCCACCTAAACATC
  CAGATAAAAATAGCAGTGTGGTCAACCCTACCTTAGTGGCTACAACCCAAATTCCATCTGTGACTTTCCC
  ATCAGCTTCCACCAAAATTACTACCCTTCCAAATGTGACTTTTCTTCCCCAGAATGCCACCACCATATCT
  TCAAGAGAAAATGTTAACACAAGCTCTTCTGTAGCTACATTAGCACCAGTGAATTCCCCAGCTCCACAAG
  ACACCACAGCTGCCCCACCCACACCTTCTGCAACTACACCAGCTCCACCATCTTCCTCAGCTCCACCAGA
  GACCACAGCTGCCCCACCCACACCTTCTGCAACTACACAAGCTCCACCATCTTCCTCAGCTCCACCAGAG
  ACCACAGCTGCCCCACCCACACCTCCTGCAACTACACCAGCTCCACCATCTTCCTCAGCTCCACCAGAGA
  CCACAGCTGCCCCACCCACACCTTCTGCAACTACACCAGCTCCACTATCTTCCTCAGCTCCACCAGAGAC
  CACAGCTGTCCCACCCACACCTTCTGCAACTACCCTAGACCCATCATCCGCCTCAGCTCCACCAGAGACC
  ACAGCTGCCCCACCCACACCTTCTGCAACTACACCAGCTCCACCGTCTTCCCCAGCTCCACAAGAGACCA
  CAGCTGCCCCAATTACCACACCTAATTCTTCCCCAACTACTCTTGCACCTGACACTTCTGAAACTTCAGC
  TGCACCCACACACCAGACTACTACTTCGGTCACTACTCAAACTACTACTACTAAACAACCAACTTCAGCT
  CCTGGCCAAAATAAAATTTCTCGATTTCTTTTATATATGAAGAATCTACTAAACAGAATTATTGACGACA
  TGGTGGAGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227257 representing NM_001145006
  Red=Cloning site Green=Tags(s)
  
  MKTLPLFVCICALSACFSFSEGRERDHELRHRRHHHQSPKSHFELPHYPGLLAHQKPFIRKSYKCLHKRC
  RPKLPPSPNNPPKFPNPHQPPKHPDKNSSVVNPTLVATTQIPSVTFPSASTKITTLPNVTFLPQNATTIS
  SRENVNTSSSVATLAPVNSPAPQDTTAAPPTPSATTPAPPSSSAPPETTAAPPTPSATTQAPPSSSAPPE
  TTAAPPTPPATTPAPPSSSAPPETTAAPPTPSATTPAPLSSSAPPETTAVPPTPSATTLDPSSASAPPET
  TAAPPTPSATTPAPPSSPAPQETTAAPITTPNSSPTTLAPDTSETSAAPTHQTTTSVTTQTTTTKQPTSA
  PGQNKISRFLLYMKNLLNRIIDDMVEQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145006
• ORF Size: 1131 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145006.1, NP_001138478.1
• RefSeq Size: 2565 bp
• RefSeq ORF: 1134 bp
• Locus ID: 4589
• UniProt ID: Q8TAX7
• Cytogenetics: 4q13.3
• Protein Families: Secreted Protein
• MW: 39.6 kDa
• Gene Summary: This gene encodes a small salivary mucin, which is thought to play a role in facilitating the clearance of bacteria in the oral cavity and to aid in mastication, speech, and swallowing. The central domain of this glycoprotein contains tandem repeats, each composed of 23 amino acids. This antimicrobial protein has antibacterial and antifungal activity. The most common allele contains 6 repeats, and some alleles may be associated with susceptibility to asthma. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Oct 2014]