C8orf48 (NM_001007090) Human Tagged ORF Clone

CAT#: RC227203

C8orf48 (Myc-DDK-tagged)-Human chromosome 8 open reading frame 48 (C8orf48)

Product Images

Circular map for RC227203

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  Western blot validation of overexpression lysate (Cat# LY425205) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227203 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C8orf48
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227203 representing NM_001007090
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATCTGCCCAGAATTGGCCCAAACGGACAAAAGTGCTCTTGCAAACCTTTCTGATGAGACTGAGA
  CTTTGAAGAACTTTACTGATGAGGTACAGACTTCCAGCTCATTCAGCTCCTCTGGAGGACGGCAGTCCTC
  GCCCCTGACCTCTGGGAGCAAACTGGAGAGGGAAAAGCAGACTCCAAGCTTGGAACAAGGAGACACACAA
  TCTGAGCTTTTGGACTATAAAAATTATGAAAAGAAGTTGAGTAAAAAATGGATCAACTACCTCAAGCTCA
  AAGACTCTAACTTTGAACGGCACCAACCAGACACCAAACTTCCAACAGAAATCACTCGGGTATCAGATGA
  AGAATTGAATGCCCTGCAGTCTTATTGCACCATGAAGATAAATTTGATTCATCGTAGAGGGGATTCTAAG
  AAGAAGACGAGCAGCAGACATAAAAAGCTGCATCTTGGATTGGATGTAGAGGCTTCAGAGAGAGATGCCT
  TTAGTTGTACTGTACCCGATGAACTTTTGAACAGAATCTACTTTAAAAACATGAGGACAACGCCAAAACA
  GGAGGCAGCAGCTAAGCAACACATATCTTATCAGTGTCCCTATTGTAACAGGAAAAGAGCGGAGCTGGCC
  CTGTCTGCCTTTCTGAAACAAAAGAAGACTTTACTGGAGTCATTTCTACTCCAAGAGAGAATAGATGAAC
  ATCTTCATACCAAAGACTTTCTCACCCGTATTGGAGAAGCACATCAAGACTTTCCCAGGCTTTCAGATGA
  CCCCAGAATAATCTGGAAAAGACTGACTGAGAAAAGTCATATCAGATACTCTGGTTTTGAAAGATCAGAG
  ACAGAGCAGAAGATGCAGCGAGATGGAAATAGTGCTTGTCATTTACCATTTTCTCTGCCATTTCTCAAGC
  GACTTACTCTAATCAAACCAGAGCTGGTGATTGTTAATGATAATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227203 representing NM_001007090
  Red=Cloning site Green=Tags(s)
  
  MAICPELAQTDKSALANLSDETETLKNFTDEVQTSSSFSSSGGRQSSPLTSGSKLEREKQTPSLEQGDTQ
  SELLDYKNYEKKLSKKWINYLKLKDSNFERHQPDTKLPTEITRVSDEELNALQSYCTMKINLIHRRGDSK
  KKTSSRHKKLHLGLDVEASERDAFSCTVPDELLNRIYFKNMRTTPKQEAAAKQHISYQCPYCNRKRAELA
  LSAFLKQKKTLLESFLLQERIDEHLHTKDFLTRIGEAHQDFPRLSDDPRIIWKRLTEKSHIRYSGFERSE
  TEQKMQRDGNSACHLPFSLPFLKRLTLIKPELVIVNDNV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007090
• ORF Size: 957 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007090.2, NP_001007091.1
• RefSeq ORF: 960 bp
• Locus ID: 157773
• UniProt ID: Q96LL4
• Cytogenetics: 8p22
• MW: 36.7 kDa