SEC13L1 (SEC13) (NM_001136232) Human Tagged ORF Clone

CAT#: RC226933

SEC13 (Myc-DDK-tagged)-Human SEC13 homolog (S. cerevisiae) (SEC13), transcript variant 2

Product Images

Circular map for RC226933

• 

  Western blot validation of overexpression lysate (Cat# LY427864) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226933 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SEC13L1
• Synonyms: D3S1231E; npp-20; SEC13L1; SEC13R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226933 representing NM_001136232
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCACGACGCCCAGATGGACTACTATGGCACCCGCCTGGCAACCTGCTCATCAGACAGGTCCGTCA
  AAATCTTTGATGTGCGCAATGGAGGGCAGATCCTTATCGCCGACCTCAGGGGTCATGAGGGTCCTGTGTG
  GCAAGTGGCCTGGGCTCACCCCATGTACGGCAACATCCTGGCATCGTGCTCCTATGACCGGAAAGTCATT
  ATCTGGAGAGAGGAAAACGGCACCTGGGAGAAGAGCCACGAGCATGCGGGACACGACTCCTCAGTGAACT
  CGGTGTGCTGGGCCCCCCATGACTACGGCCTGATCCTGGCCTGTGGGAGCTCGGATGGGGCCATCTCCCT
  GCTGACTTACACCGGGGAAGGCCAATGGGAAGTAAAGAAGATCAACAACGCTCACACCATTGGCTGCAAT
  GCCGTCAGCTGGGCCCCTGCTGTTGTACCTGGAAGCCTCATAGACCACCCATCGGGGCAGAAACCCAATT
  ACATCAAGAGGTTTGCATCAGGTGGCTGTGACAACCTCATCAAGCTGTGGAAGGAGGAGGAGGACGGCCA
  GTGGAAGGAGGAGCAGAAGCTAGAAGCGCACAGTGACTGGGTTCGAGATGTGGCCTGGGCCCCCTCCATC
  GGCCTGCCCACCAGCACCATCGCCAGCTGCTCCCAGGATGGTCGTGTGTTCATTTGGACCTGTGATGATG
  CCTCAAGCAATACGTGGTCCCCTAAATTGTTGCACAAGTTCAACGATGTGGTGTGGCATGTGAGCTGGTC
  CATCACAGCCAACATCCTGGCTGTCTCTGGTGGAGACAATAAGGTGACCCTGTGGAAGGAGTCAGTTGAT
  GGGCAGTGGGTGTGCATCAGTGATGTCAACAAGGGCCAGGGCTCCGTATCAGCATCAGTGACAGAGGGCC
  AGCAGAACGAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226933 representing NM_001136232
  Red=Cloning site Green=Tags(s)
  
  MIHDAQMDYYGTRLATCSSDRSVKIFDVRNGGQILIADLRGHEGPVWQVAWAHPMYGNILASCSYDRKVI
  IWREENGTWEKSHEHAGHDSSVNSVCWAPHDYGLILACGSSDGAISLLTYTGEGQWEVKKINNAHTIGCN
  AVSWAPAVVPGSLIDHPSGQKPNYIKRFASGGCDNLIKLWKEEEDGQWKEEQKLEAHSDWVRDVAWAPSI
  GLPTSTIASCSQDGRVFIWTCDDASSNTWSPKLLHKFNDVVWHVSWSITANILAVSGGDNKVTLWKESVD
  GQWVCISDVNKGQGSVSASVTEGQQNEQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136232
• ORF Size: 924 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136232.2, NP_001129704.1
• RefSeq ORF: 927 bp
• Locus ID: 6396
• UniProt ID: P55735
• Cytogenetics: 3p25.3
• MW: 33.8 kDa
• Gene Summary: The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Oct 2008]