NFAT5 (NM_001113178) Human Tagged ORF Clone

CAT#: RC226407

NFAT5 (Myc-DDK-tagged)-Human nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant 6

Product Images

Circular map for RC226407

• 

  Western blot validation of overexpression lysate (Cat# LY426386) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226407 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NFAT5
• Synonyms: NF-AT5; NFATL1; NFATZ; OREBP; TONEBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226407 representing NM_001113178
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCGGACTTCATCTCATTGCTCAGCGCGGACCTAGACCTGGAATCGCCCAAGTCCCTCTACTCGC
  GAGATTCTCTGAAGTTACACCCATCACAGAATTTTCATAGAGCTGGACTATTGGAAGAATCTGTCTATGA
  TCTTCTCCCAAAGGAGTTACAGTTACCTCCATCTAGAGAAACATCTGTAGCATCAATGAGTCAGACAAGC
  GGTGGTGAGGCAGGCTCGCCTCCTCCAGCTGTTGTTGCTGCTGATGCTTCTTCAGCTCCCTCCTCTTCCT
  CCATGGGCGGTGCTTGCAGCTCCTTTACCACCTCTTCCAGCCCTACCATTTATTCTACCTCAGTCACCGA
  CAGCAAGGCTATGCAAGTGGAGAGCTGCTCCTCAGCCGTGGGGGTAAGTAACAGAGGGGTAAGTGAAAAG
  CAGTTAACCAGTAACACAGTTCAGCAGCATCCATCAACACCGAAGAGGCACACAGTCTTGTACATCTCAC
  CACCACCTGAGGACTTGCTGGATAACAGTCGGATGTCCTGCCAGGATGAGGGGTGTGGATTGGAATCTGA
  GCAGAGCTGCAGTATGTGGATGGAGGATTCCCCCTCCAACTTCAGTAACATGAGCACCAGTTCCTACAAT
  GATAACACTGAGGTACCTCGTAAATCACGAAAACGAAATCCAAAGCAGAGGCCGGGGGTCAAACGACGAG
  ATTGTGAAGAATCTAATATGGATATATTTGATGCCGACAGTGCCAAAGCACCTCACTATGTGCTTTCTCA
  GCTTACCACGGACAACAAAGGCAACTCAAAAGCGGGAAATGGAACATTGGAAAACCAAAAAGGAACTGGA
  GTAAAGAAGAGCCCTATGTTGTGTGGACAATATCCTGTTAAAAGTGAGGGAAAGGAGCTGAAGATAGTTG
  TACAACCTGAGACACAGCACCGAGCTCGGTACCTGACTGAGGGCAGCCGTGGCTCAGTGAAAGATAGAAC
  ACAGCAAGGCTTTCCTACAGTAAAGCTGGAAGGCCATAATGAACCTGTAGTGTTGCAAGTGTTTGTGGGC
  AACGACTCTGGACGAGTGAAACCACATGGATTTTATCAGGCCTGCAGAGTAACTGGACGAAATACAACTC
  CTTGCAAAGAAGTGGACATTGAAGGCACTACTGTTATAGAAGTCGGCCTTGATCCTAGCAACAACATGAC
  ACTGGCGGTGGACTGCGTAGGGATATTGAAATTGAGGAATGCTGATGTCGAAGCCAGAATAGGAATTGCT
  GGTTCCAAGAAGAAAAGCACTCGTGCCAGATTGGTTTTTCGAGTTAATATCATGAGGAAAGATGGCTCCA
  CTTTGACACTGCAAACACCCTCTTCTCCAATTTTGTGTACTCAGCCAGCAGGAGTGCCAGAAATCTTAAA
  GAAAAGCTTGCATAGCTGTTCAGTGAAAGGAGAAGAAGAAGTGTTTTTAATCGGCAAGAACTTTCTGAAA
  GGAACTAAAGTTATTTTCCAAGAAAATGTTTCTGATGAAAACTCTTGGAAGTCAGAAGCTGAAATTGATA
  TGGAACTATTTCATCAGAATCATCTTATTGTGAAGGTTCCTCCCTATCATGACCAACATATAACTTTGCC
  TGTGTCAGTGGGAATATATGTAGTGACAAATGCTGGAAGATCTCATGATGTTCAACCATTCACTTACACT
  CCAGACCCAGCAGCTGGTGCTTTGAATGTAAATGTGAAGAAGGAAATATCTAGTCCAGCAAGACCTTGCT
  CTTTTGAAGAGGCCATGAAAGCAATGAAAACTACTGGATGTAATTTAGATAAGGTAAATATTATCCCTAA
  TGCCCTGATGACTCCACTCATACCAAGCAGTATGATTAAGAGTGAAGATGTTACTCCAATGGAAGTAACA
  GCAGAAAAAAGATCTTCCACTATTTTTAAGACTACAAAGTCTGTTGGATCAACTCAGCAAACATTAGAAA
  ACATCTCAAACATAGCAGGAAATGGCTCTTTTTCATCACCATCATCTTCCCACCTACCTTCTGAAAATGA
  AAAACAGCAGCAGATTCAGCCCAAGGCATACAACCCAGAGACCCTGACAACTATTCAAACCCAGGACATC
  TCACAGCCTGGTACTTTTCCAGCAGTTTCTGCTTCTAGTCAGCTGCCCAACAGCGATGCACTATTGCAGC
  AGGCTACACAGTTTCAGACAAGAGAAACTCAGTCTAGAGAGATATTACAGTCAGATGGTACAGTGGTTAA
  TTTGTCACAACTGACTGAGGCATCACAACAACAGCAGCAGTCACCACTACAAGAACAAGCACAGACTTTA
  CAGCAGCAGATTTCATCAAATATTTTTCCATCACCAAATAGTGTGAGTCAGCTTCAGAATACTATTCAGC
  AGCTGCAAGCAGGGAGTTTCACAGGCAGTACTGCTAGTGGCAGCAGTGGAAGTGTTGACTTGGTCCAACA
  AGTTTTAGAGGCACAGCAGCAGTTATCTTCAGTTTTATTTTCTGCTCCAGATGGTAATGAGAATGTTCAA
  GAGCAGCTTAGTGCAGATATTTTTCAACAAGTCAGTCAAATTCAGAGTGGTGTAAGCCCTGGAATGTTTT
  CCTCAACAGAGCCAACAGTCCATACCAGACCAGATAATTTATTACCTGGAAGAGCTGAAAGTGTTCATCC
  ACAGTCTGAAAACACGTTATCTAATCAACAGCAGCAGCAGCAGCAGCAACAGCAAGTGATGGAATCTTCA
  GCCGCAATGGTGATGGAGATGCAACAGAGTATCTGCCAGGCAGCTGCCCAGATTCAGTCAGAGTTATTCC
  CTTCAACTGCTTCAGCAAATGGAAACCTTCAGCAATCGCCAGTTTACCAGCAGACTTCTCACATGATGAG
  TGCATTGTCTACCAATGAGGATATGCAAATGCAGTGTGAATTGTTTTCTTCTCCTCCTGCAGTTTCTGGA
  AATGAAACTTCTACAACTACCACACAGCAGGTTGCAACCCCTGGCACTACCATGTTTCAGACATCAAGTT
  CAGGAGATGGAGAAGAAACTGGAACACAAGCAAAACAGATTCAGAACAGTGTCTTTCAGACCATGGTCCA
  AATGCAACATAGTGGGGACAATCAACCTCAAGTTAACCTTTTTTCATCCACAAAAAGTATGATGAGTGTT
  CAGAATAGTGGTACCCAACAACAAGGTAATGGTTTATTCCAGCAAGGGAATGAGATGATGTCACTTCAAT
  CTGGAAATTTTTTGCAGCAGTCTTCTCATTCACAGGCCCAACTTTTTCATCCTCAAAATCCTATTGCCGA
  TGCTCAGAACCTTTCCCAGGAAACTCAAGGTTCTCTCTTTCATAGTCCAAATCCTATTGTCCACAGTCAG
  ACTTCTACAACCTCCTCTGAACAAATGCAGCCTCCAATGTTTCACTCTCAAAGTACCATTGCTGTGTTAC
  AGGGCTCTTCAGTTCCTCAAGACCAGCAGTCAACCAACATATTTCTTTCCCAGAGTCCCATGAATAATCT
  TCAGACTAACACAGTAGCCCAAGAAGCATTTTTTGCAGCACCGAACTCAATTTCTCCACTTCAGTCAACA
  TCAAACAGTGAACAACAAGCTGCTTTCCAACAGCAAGCTCCAATATCACACATCCAGACTCCTATGCTTT
  CCCAAGAACAGGCACAACCCCCGCAGCAGGGTTTATTTCAGCCTCAGGTGGCCCTGGGCTCCCTTCCACC
  TAATCCAATGCCTCAAAGCCAACAAGGAACCATGTTCCAGTCACAGCACTCAATAGTTGCCATGCAGAGT
  AACTCTCCATCCCAGGAACAGCAGCAGCAGCAGCAACAGCAGCAGCAACAGCAGCAGCAACAACAACAGA
  GCATTTTATTCAGTAATCAGAATACCATGGCTACAATGGCGTCTCCAAAGCAACCACCACCAAACATGAT
  ATTCAACCCAAATCAAAATCCAATGGCTAATCAGGAGCAACAGAACCAGTCAATTTTTCACCAACAAAGT
  AACATGGCCCCAATGAATCAAGAGCAACAGCCCATGCAATTTCAGAGTCAGTCCACAGTTTCCTCACTTC
  AGAACCCAGGTCCTACCCAGTCGGAATCATCACAGACCCCCTTGTTCCATAGCTCTCCTCAGATTCAGTT
  GGTACAAGGGTCACCTAGTTCTCAAGAGCAGCAAGTAACTCTCTTCTTATCTCCAGCATCCATGTCTGCC
  TTGCAGACCAGTATAAATCAACAAGATATGCAACAGTCTCCTCTTTATTCCCCTCAGAACAACATGCCTG
  GAATTCAAGGAGCCACATCTTCGCCTCAACCACAGGCTACTTTATTTCACAACACAGCAGGAGGCACAAT
  GAACCAACTGCAGAATTCTCCTGGCTCATCTCAGCAGACATCAGGAATGTTCTTATTTGGCATTCAAAAT
  AACTGTAGTCAGCTTTTAACCTCTGGACCAGCTACATTGCCTGATCAGTTGATGGCCATAAGTCAGCCAG
  GCCAACCACAAAACGAGGGCCAGCCACCTGTGACAACACTTCTTTCTCAGCAAATGCCAGAGAATTCTCC
  ACTGGCATCCTCTATAAACACCAACCAGAACATCGAAAAGATTGATTTGCTTGTTTCATTGCAAAACCAA
  GGGAACAACTTGACTGGCTCCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226407 representing NM_001113178
  Red=Cloning site Green=Tags(s)
  
  MPSDFISLLSADLDLESPKSLYSRDSLKLHPSQNFHRAGLLEESVYDLLPKELQLPPSRETSVASMSQTS
  GGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEK
  QLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYN
  DNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTG
  VKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVG
  NDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIA
  GSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLK
  GTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYT
  PDPAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVT
  AEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDI
  SQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTL
  QQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQ
  EQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESS
  AAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSG
  NETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSV
  QNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQ
  TSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQST
  SNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQS
  NSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQS
  NMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSA
  LQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQN
  NCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQ
  GNNLTGSF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113178
• ORF Size: 4644 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113178.3
• RefSeq ORF: 4647 bp
• Locus ID: 10725
• UniProt ID: O94916
• Cytogenetics: 16q22.1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Axon guidance, B cell receptor signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• MW: 167.6 kDa
• Gene Summary: The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]