PRAGMIN (PRAG1) (NM_001080826) Human Tagged ORF Clone

CAT#: RC226391

SGK223 (Myc-DDK-tagged)-Human homolog of rat pragma of Rnd2 (SGK223)

Product Images

Circular map for RC226391

• 

  Western blot validation of overexpression lysate (Cat# LY425920) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226391 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PRAG1 protein (Cat# TP326391). The protein was produced from HEK293T cells transfected with PRAG1 cDNA clone (Cat# RC226391) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRAGMIN
• Synonyms: NACK; PEAK2; PRAGMIN; SGK223
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226391 representing NM_001080826
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCAGACCCTCTGCCTGAACCCCGAGAGCCTGAAAATGTCTGCGTGCAGTGACTTTGTGGAGCACA
  TCTGGAAACCCGGGTCCTGCAAGAACTGCTTCTGCCTGCGGAGCGACCACCAGCTGGTGGCCGGCCCTCC
  CCAGCCCAGAGCGGGCAGCCTGCCCCCTCCACCGCGCCTGCCTCCCAGGCCTGAGAACTGCCGCCTGGAA
  GATGAAGGTGTGAACAGCTCACCTTACTCCAAGCCCACAATTGCCGTGAAGCCCACCATGATGAGCTCCG
  AGGCCTCTGATGTGTGGACAGAGGCCAACCTGAGTGCCGAAGTCTCGCAGGTCATCTGGAGACGAGCCCC
  TGGCAAGCTCCCCCTCCCGAAGCAGGAGGATGCCCCCGTCGTCTACCTGGGCAGCTTCCGAGGTGTACAG
  AAGCCTGCTGGTCCCTCTACCTCCCCTGATGGCAATTCTCGCTGTCCCCCAGCTTACACCATGGTCGGCC
  TGCACAACCTTGAGCCCCGCGGCGAGAGGAACATTGCCTTCCACCCGGTGAGCTTCCCGGAGGAGAAGGC
  TGTGCACAAAGAAAAACCCTCATTTCCTTACCAAGACCGGCCCTCCACCCAGGAGAGCTTCCGCCAGAAA
  CTGGCTGCCTTTGCTGGGACCACATCTGGCTGTCACCAGGGCCCTGGGCCCCTGCGGGAATCCCTGCCCT
  CGGAGGATGACAGTGATCAAAGGTGCTCGCCCTCCGGGGACAGCGAGGGTGGAGAGTACTGCTCCATCCT
  GGACTGCTGCCCTGGGAGCCCTGTTGCCAAGGCTGCCTCCCAGACTGCAGGTTCCCGGGGCAGGCATGGT
  GGCAGGGACTGCTCACCCACGTGCTGGGAGCAGGGGAAGTGTTCCGGGCCCGCAGAGCAGGAGAAGCGGG
  GCCCGAGCTTCCCCAAGGAGTGCTGTAGCCAGGGCCCCACTGCCCACCCATCCTGCCTGGGCCCCAAGAA
  ACTGTCCCTCACCTCGGAGGCTGCCATTTCTTCCGACGGCCTCTCTTGTGGCAGCGGCAGCGGCAGCGGC
  GCCAGTAGCCCCTTCGTCCCCCACCTCGAGAGTGATTACTGCTCCCTCATGAAGGAACCTGCCCCAGAGA
  AGCAGCAGGACCCTGGCTGCCCAGGGGTGACCCCTAGCAGATGCCTTGGGCTGACGGGGGAGCCCCAGCC
  CCCGGCCCACCCCCGGGAGGCTACACAGCCTGAACCCATCTATGCTGAGAGCACCAAGAGGAAGAAGGCA
  GCTCCGGTGCCTTCCAAGTCACAGGCCAAGATAGAACATGCAGCTGCTGCCCAGGGCCAAGGCCAGGTAT
  GCACAGGTAATGCCTGGGCCCAGAAAGCAGCATCTGGCTGGGGCCGGGACAGCCCAGACCCAACTCCCCA
  GGTGTCAGCCACCATCACAGTCATGGCGGCCCACCCGGAAGAGGACCATCGGACGATCTACCTGAGCAGC
  CCTGACTCTGCAGTGGGGGTGCAGTGGCCACGAGGGCCTGTGAGCCAGAACTCCGAGGTAGGTGAAGAGG
  AGACTTCGGCTGGGCAGGGGCTGAGCTCCAGGGAAAGCCATGCTCACAGTGCCAGCGAGAGCAAGCCCAA
  GGAGAGGCCCGCCATTCCCCCCAAGTTGTCCAAGAGTAGCCCTGTAGGGTCCCCGGTGTCACCGTCTGCT
  GGAGGGCCCCCAGTGTCACCGCTGGCTGACCTTAGTGATGGGAGCTCTGGCGGCAGCAGCATTGGGCCCC
  AGCCTCCATCCCAAGGTCCTGCTGACCCCGCTCCTTCCTGCCGGACCAACGGTGTCGCTATCAGTGACCC
  ATCCAGGTGTCCCCAGCCTGCCGCCTCGTCAGCCTCGGAACAGAGGCGGCCCAGGTTCCAGGCAGGCACC
  TGGAGTCGTCAGTGCCGGATAGAGGAAGAAGAGGAGGTGGAGCAGGAATTGCTGAGTCACAGCTGGGGAA
  GAGAGACCAAAAATGGCCCCACGGACCATTCAAACTCCACGACCTGGCACCGTCTCCACCCCACAGATGG
  CTCCTCTGGGCAGAACAGCAAAGTTGGGACCGGGATGAGCAAATCCGCCTCTTTTGCCTTTGAGTTCCCC
  AAGGACAGAAGTGGGATTGAGACATTCTCACCTCCTCCTCCGCCTCCAAAGTCGCGGCACCTTCTAAAAA
  TGAACAAGAGCAGCTCTGATTTGGAAAAAGTGAGCCAGGGCTCTGCAGAAAGCCTCAGCCCATCCTTCAG
  GGGTGTCCACGTCAGCTTCACCACCGGCTCCACGGACAGCCTGGCCTCAGACTCTAGGACCTGCAGCGAT
  GGAGGTCCCTCGTCTGAGCTGGCTCACTCGCCCACCAACAGCGGGAAGAAGCTCTTTGCTCCCGTTCCGT
  TTCCTTCAGGCTCCACTGAGGACGTGTCCCCCAGTGGCCCCCAGCAGCCCCCTCCACTCCCCCAGAAAAA
  GATAGTGAGCCGGGCAGCCTCTTCACCGGATGGCTTCTTCTGGACCCAAGGCTCCCCCAAGCCCGGAACA
  GCAAGCCCCAAGCTGAACCTAAGCCACTCGGAAACCAACGTCCACGACGAATCTCACTTTAGCTATTCGT
  TGAGCCCCGGGAACCGCCACCATCCTGTCTTCTCCTCTTCCGATCCTCTGGAGAAAGCTTTCAAAGGCAG
  TGGCCACTGGCTTCCGGCAGCAGGGCTGGCGGGCAACAGAGGCGGCTGCGGGAGCCCTGGCCTCCAGTGC
  AAAGGGGCCCCCTCCGCCTCATCCTCCCAGCTGAGCGTGTCCAGTCAAGCCTCCACCGGGAGCACCCAGC
  TTCAGCTGCACGGTCTCCTGAGCAACATCAGCAGCAAGGAGGGCACCTATGCCAAGCTGGGGGGACTCTA
  CACCCAGTCCCTGGCCCGCCTTGTAGCCAAATGTGAGGACCTCTTCATGGGCGGCCAGAAAAAGGAGCTC
  CACTTCAATGAGAATAACTGGTCGCTCTTCAAGCTGACTTGTAACAAGCCCTGCTGTGACTCGGGGGATG
  CCATTTATTACTGTGCCACCTGCTCTGAGGACCCCGGCAGCACCTATGCTGTGAAAATCTGCAAAGCCCC
  TGAGCCCAAAACAGTCTCCTACTGCAGCCCGTCCGTGCCCGTGCACTTTAACATCCAGCAGGACTGCGGC
  CACTTCGTCGCCTCGGTGCCGTCCAGCATGCTCAGCTCCCCCGACGCGCCCAAGGACCCTGTGCCTGCCC
  TGCCCACACACCCCCCTGCCCAGGAGCAGGACTGCGTGGTGGTCATCACCCGAGAGGTGCCACATCAGAC
  CGCCTCCGACTTCGTGCGGGACTCGGCGGCCAGCCACCAGGCGGAGCCCGAGGCGTACGAGCGGCGCGTG
  TGCTTCCTGCTTCTGCAACTCTGCAACGGGCTGGAGCACCTGAAGGAGCACGGGATCATCCACCGGGACC
  TGTGCCTGGAGAACCTGCTGCTGGTGCACTGCACCCTCCAGGCCGGCCCCGGGCCCGCCCCCGCCCCGGC
  TCCCGCCCCCGCCGCCGCCGCGCCTCCCTGCTCCTCTGCCGCCCCGCCTGCTGGTGGCACTCTCAGCCCC
  GCAGCCGGCCCCGCCTCCCCGGAAGGGCCCCGGGAGAAGCAGCTGCCCCGGCTCATCATCAGCAACTTTT
  TGAAGGCCAAGCAGAAGCCGGGCGGCACCCCAAACCTGCAGCAGAAGAAGAGCCAGGCCCGGCTGGCCCC
  CGAGATCGTGTCTGCTTCCCAGTACCGCAAGTTCGATGAGTTCCAGACAGGCATCCTCATCTACGAGCTG
  CTGCACCAACCCAACCCGTTCGAGGTGCGCGCCCAGCTGCGGGAGAGAGACTACCGGCAGGAGGACCTGC
  CGCCGCTGCCCGCGCTGTCCCTCTACTCACCCGGCCTGCAGCAGCTGGCACATCTGCTACTGGAGGCCGA
  CCCCATCAAGCGTATCCGCATCGGCGAGGCCAAGCGCGTGCTGCAGTGCCTGCTGTGGGGGCCTCGGCGC
  GAGCTGGTGCAGCAGCCGGGCACCTCGGAGGAGGCGCTGTGCGGCACGCTGCACAACTGGATCGACATGA
  AGCGGGCCCTGATGATGATGAAGTTTGCGGAGAAGGCGGTGGATCGCAGGCGGGGCGTGGAGCTGGAGGA
  CTGGCTTTGCTGCCAGTACCTGGCGTCTGCGGAGCCCGGGGCCCTCTTACAGTCGCTGAAGCTCCTGCAG
  CTTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226391 representing NM_001080826
  Red=Cloning site Green=Tags(s)
  
  MHQTLCLNPESLKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVAGPPQPRAGSLPPPPRLPPRPENCRLE
  DEGVNSSPYSKPTIAVKPTMMSSEASDVWTEANLSAEVSQVIWRRAPGKLPLPKQEDAPVVYLGSFRGVQ
  KPAGPSTSPDGNSRCPPAYTMVGLHNLEPRGERNIAFHPVSFPEEKAVHKEKPSFPYQDRPSTQESFRQK
  LAAFAGTTSGCHQGPGPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPVAKAASQTAGSRGRHG
  GRDCSPTCWEQGKCSGPAEQEKRGPSFPKECCSQGPTAHPSCLGPKKLSLTSEAAISSDGLSCGSGSGSG
  ASSPFVPHLESDYCSLMKEPAPEKQQDPGCPGVTPSRCLGLTGEPQPPAHPREATQPEPIYAESTKRKKA
  APVPSKSQAKIEHAAAAQGQGQVCTGNAWAQKAASGWGRDSPDPTPQVSATITVMAAHPEEDHRTIYLSS
  PDSAVGVQWPRGPVSQNSEVGEEETSAGQGLSSRESHAHSASESKPKERPAIPPKLSKSSPVGSPVSPSA
  GGPPVSPLADLSDGSSGGSSIGPQPPSQGPADPAPSCRTNGVAISDPSRCPQPAASSASEQRRPRFQAGT
  WSRQCRIEEEEEVEQELLSHSWGRETKNGPTDHSNSTTWHRLHPTDGSSGQNSKVGTGMSKSASFAFEFP
  KDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSD
  GGPSSELAHSPTNSGKKLFAPVPFPSGSTEDVSPSGPQQPPPLPQKKIVSRAASSPDGFFWTQGSPKPGT
  ASPKLNLSHSETNVHDESHFSYSLSPGNRHHPVFSSSDPLEKAFKGSGHWLPAAGLAGNRGGCGSPGLQC
  KGAPSASSSQLSVSSQASTGSTQLQLHGLLSNISSKEGTYAKLGGLYTQSLARLVAKCEDLFMGGQKKEL
  HFNENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKAPEPKTVSYCSPSVPVHFNIQQDCG
  HFVASVPSSMLSSPDAPKDPVPALPTHPPAQEQDCVVVITREVPHQTASDFVRDSAASHQAEPEAYERRV
  CFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCTLQAGPGPAPAPAPAPAAAAPPCSSAAPPAGGTLSP
  AAGPASPEGPREKQLPRLIISNFLKAKQKPGGTPNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYEL
  LHQPNPFEVRAQLRERDYRQEDLPPLPALSLYSPGLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRR
  ELVQQPGTSEEALCGTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPGALLQSLKLLQ
  LL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080826
• ORF Size: 4206 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080826.1, NP_001074295.1
• RefSeq ORF: 4221 bp
• Locus ID: 157285
• UniProt ID: Q86YV5
• Cytogenetics: 8p23.1
• Protein Families: Protein Kinase
• MW: 149.1 kDa
• Gene Summary: This gene encodes an enzyme that belongs to the tyrosine protein kinase family. A similar protein in rat binds to Rho family GTPase 2 (Rnd2) and regulates neurite outgrowth via activation of Ras homolog gene family, member A (RhoA). [provided by RefSeq, Mar 2014]