LTBP3 (NM_001130144) Human Tagged ORF Clone

CAT#: RC226379

LTBP3 (Myc-DDK-tagged)-Human latent transforming growth factor beta binding protein 3 (LTBP3), transcript variant 1

Product Images

Circular map for RC226379

• 

  Western blot validation of overexpression lysate (Cat# LY427180) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226379 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified LTBP3 protein (Cat# TP326379). The protein was produced from HEK293T cells transfected with LTBP3 cDNA clone (Cat# RC226379) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LTBP3
• Synonyms: DASS; GPHYSD3; LTBP-3; LTBP2; pp6425; STHAG6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226379 representing NM_001130144
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGGGCCCCGAGGGGCTGCTGGCGGCCTGGCCCCTGAGATGCGCGGGGCGGGGGCGGCGGGGCTGC
  TGGCGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGGGCCTGGGCGGCAGGGTCGAGGGGGGGCCGGCCGG
  CGAGCGGGGCGCAGGCGGGGGCGGGGCGCTGGCCCGCGAGCGCTTCAAGGTGGTCTTTGCGCCGGTGATC
  TGCAAGCGGACCTGTCTCAAGGGCCAGTGTCGGGACAGTTGTCAGCAGGGCTCCAACATGACGCTCATCG
  GAGAGAACGGCCACAGCACAGACACGCTCACGGGCTCCGGCTTCCGCGTGGTGGTGTGCCCTCTCCCCTG
  CATGAATGGCGGCCAGTGCTCCTCGCGAAACCAGTGCCTGTGTCCCCCGGACTTCACTGGGCGCTTCTGC
  CAGGTGCCCGCAGGAGGAGCCGGTGGGGGTACCGGCGGCTCAGGCCCCGGCCTGAGCAGGACAGGGGCCC
  TGTCCACAGGGGCGCTGCCGCCCCTGGCTCCGGAGGGCGACTCTGTGGCCAGCAAGCACGCCATCTACGC
  CGTCCAGGTGATCGCTGACCCTCCTGGGCCCGGGGAGGGGCCTCCTGCCCAGCACGCAGCCTTCCTGGTG
  CCCCTAGGCCCGGGACAGATCTCAGCAGAAGTGCAGGCCCCGCCCCCCGTGGTGAATGTGCGCGTCCATC
  ACCCGCCCGAGGCCTCAGTCCAGGTGCACCGCATTGAGAGCTCGAACGCCGAGAGCGCAGCCCCCTCCCA
  GCACCTGCTGCCGCACCCCAAGCCCTCGCACCCCCGGCCGCCCACCCAGAAGCCCCTGGGCCGCTGCTTT
  CAGGACACTCTGCCCAAGCAGCCGTGTGGCAGCAACCCCCTCCCCGGCCTCACCAAGCAGGAAGACTGCT
  GCGGTAGCATCGGCACTGCCTGGGGCCAGAGCAAGTGCCACAAGTGTCCCCAGCTGCAGTACACAGGAGT
  GCAGAAGCCAGGGCCTGTACGTGGGGAAGTGGGCGCTGACTGTCCCCAGGGCTACAAGAGGCTTAACAGC
  ACCCACTGCCAGGACATCAACGAGTGCGCAATGCCGGGCGTGTGTCGCCATGGTGACTGCCTCAACAACC
  CTGGCTCCTATCGCTGTGTCTGCCCACCTGGCCATAGTTTAGGCCCCTCCCGTACACAGTGCATTGCAGA
  CAAACCGGAGGAGAAGAGCCTGTGTTTCCGCCTGGTGAGCCCTGAGCACCAGTGCCAGCACCCACTGACC
  ACCCGCCTGACCCGCCAGCTCTGCTGCTGCAGTGTCGGCAAGGCCTGGGGCGCGCGGTGTCAGCGCTGCC
  CAACAGATGGCACCGCTGCGTTCAAGGAGATCTGCCCAGCTGGGAAGGGATACCACATTCTCACCTCCCA
  CCAGACGCTCACCATTCAGGGCGAGAGTGACTTTTCCCTTTTCCTGCACCCTGACGGGCCACCCAAGCCC
  CAGCAGCTTCCGGAGAGCCCTAGCCAGGCTCCACCACCTGAGGACACAGAGGAAGAGAGAGGGGTGACCA
  CGGACTCACCGGTGAGTGAGGAGAGGTCAGTGCAGCAGAGCCACCCAACTGCCACCACGACTCCTGCCCG
  GCCCTACCCCGAGCTGATCTCCCGTCCCTCGCCCCCGACCATGCGCTGGTTCCTGCCGGACTTGCCTCCT
  TCCCGCAGCGCCGTAGAGATCGCTCCCACTCAGGTCACAGAGACTGATGAGTGCCGACTGAACCAGAACA
  TCTGTGGCCACGGAGAGTGCGTGCCGGGCCCCCCTGACTACTCCTGCCACTGCAACCCCGGCTACCGGTC
  ACATCCCCAGCACCGCTACTGCGTGGATGTGAACGAGTGCGAGGCAGAGCCCTGTGGCCCGGGGAGGGGC
  ATCTGCATGAACACCGGCGGCTCCTACAATTGCCACTGCAACCGCGGCTACCGCCTGCACGTGGGCGCCG
  GGGGGCGCTCGTGCGTGGACCTGAACGAATGCGCCAAGCCCCACCTGTGCGGCGACGGCGGCTTCTGCAT
  CAACTTTCCCGGTCACTACAAGTGCAACTGCTACCCCGGCTACCGGCTCAAAGCCTCCCGGCCTCCTGTG
  TGCGAAGACATCGACGAGTGCCGGGACCCAAGCTCTTGCCCGGATGGCAAATGCGAGAACAAGCCCGGGA
  GCTTCAAGTGCATCGCCTGTCAGCCTGGCTACCGCAGCCAGGGGGGCGGGGCCTGTCGCGACGTGAACGA
  GTGCGCCGAGGGCAGCCCCTGCTCGCCTGGCTGGTGCGAGAACCTCCCGGGCTCCTTCCGCTGCACCTGT
  GCCCAGGGCTACGCGCCCGCGCCCGACGGCCGCAGTTGCTTGGATGTGGACGAGTGTGAGGCTGGGGACG
  TGTGTGACAATGGCATCTGCAGCAACACGCCAGGATCTTTCCAGTGTCAGTGCCTCTCTGGCTACCATCT
  GTCCAGGGACCGGAGCCACTGCGAGGACATTGATGAGTGTGACTTCCCTGCAGCCTGCATTGGGGGTGAC
  TGCATCAATACCAATGGCTCCTACAGATGTCTTTGCCCCCAGGGGCATCGGCTGGTGGGTGGCAGGAAAT
  GCCAAGACATAGATGAGTGCAGCCAGGACCCGAGCCTGTGCCTTCCCCATGGGGCCTGCAAGAACCTTCA
  GGGCTCCTATGTGTGTGTCTGCGATGAGGGCTTCACTCCCACCCAGGACCAGCACGGTTGTGAGGAGGTG
  GAGCAGCCCCACCACAAGAAGGAGTGCTACCTGAACTTCGATGACACAGTGTTCTGCGACAGCGTATTGG
  CCACCAACGTGACCCAGCAGGAGTGCTGCTGCTCTCTGGGGGCCGGCTGGGGCGACCACTGCGAAATCTA
  CCCCTGCCCAGTCTACAGCTCAGCCGAGTTCCACAGCCTCTGCCCAGACGGAAAGGGCTACACCCAGGAC
  AACAACATCGTCAACTACGGCATCCCAGCCCACCGTGACATCGACGAGTGCATGTTGTTCGGGTCGGAGA
  TTTGCAAGGAGGGCAAGTGCGTGAACACGCAGCCTGGCTACGAGTGCTACTGCAAGCAGGGCTTCTACTA
  CGACGGGAACCTGCTGGAATGCGTGGACGTGGACGAGTGCCTGGACGAGTCCAACTGCCGGAACGGAGTG
  TGTGAGAACACGCGCGGCGGCTACCGCTGTGCCTGCACGCCCCCTGCCGAGTACAGTCCCGCGCAGCGCC
  AGTGCCTGAGCCCGGAAGAGATGGACGTGGACGAGTGCCAGGACCCGGCAGCCTGCCGCCCTGGCCGCTG
  CGTCAACCTGCCGGGCTCCTACCGCTGCGAGTGTCGCCCGCCCTGGGTGCCCGGGCCCTCCGGCCGCGAT
  TGCCAGCTCCCCGAGAGCCCGGCCGAGCGTGCCCCGGAGCGGCGCGACGTGTGCTGGAGCCAGCGCGGAG
  AGGACGGCATGTGCGCTGGCCCCCTGGCCGGGCCTGCCCTCACCTTCGACGACTGCTGCTGCCGCCAGGG
  CCGCGGCTGGGGCGCCCAATGCCGACCGTGCCCGCCGCGCGGCGCGGGGTCCCATTGCCCGACATCGCAG
  AGCGAGAGCAATTCCTTCTGGGACACAAGCCCCCTGCTGTTGGGGAAGCCCCCAAGAGATGAGGACAGTT
  CAGAGGAGGATTCAGACGAGTGTCGCTGCGTGAGTGGCCGCTGCGTGCCGCGGCCGGGCGGCGCCGTGTG
  CGAGTGTCCCGGCGGCTTCCAGCTCGACGCCTCCCGCGCCCGCTGCGTGGATATCGACGAGTGCCGAGAG
  CTGAACCAGCGCGGGCTGCTGTGCAAGAGCGAGCGCTGCGTGAACACCAGCGGCTCCTTCCGCTGCGTCT
  GCAAAGCCGGCTTCGCGCGCAGCCGCCCGCACGGGGCCTGCGTTCCCCAGCGCCGCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226379 representing NM_001130144
  Red=Cloning site Green=Tags(s)
  
  MPGPRGAAGGLAPEMRGAGAAGLLALLLLLLLLLLGLGGRVEGGPAGERGAGGGGALARERFKVVFAPVI
  CKRTCLKGQCRDSCQQGSNMTLIGENGHSTDTLTGSGFRVVVCPLPCMNGGQCSSRNQCLCPPDFTGRFC
  QVPAGGAGGGTGGSGPGLSRTGALSTGALPPLAPEGDSVASKHAIYAVQVIADPPGPGEGPPAQHAAFLV
  PLGPGQISAEVQAPPPVVNVRVHHPPEASVQVHRIESSNAESAAPSQHLLPHPKPSHPRPPTQKPLGRCF
  QDTLPKQPCGSNPLPGLTKQEDCCGSIGTAWGQSKCHKCPQLQYTGVQKPGPVRGEVGADCPQGYKRLNS
  THCQDINECAMPGVCRHGDCLNNPGSYRCVCPPGHSLGPSRTQCIADKPEEKSLCFRLVSPEHQCQHPLT
  TRLTRQLCCCSVGKAWGARCQRCPTDGTAAFKEICPAGKGYHILTSHQTLTIQGESDFSLFLHPDGPPKP
  QQLPESPSQAPPPEDTEEERGVTTDSPVSEERSVQQSHPTATTTPARPYPELISRPSPPTMRWFLPDLPP
  SRSAVEIAPTQVTETDECRLNQNICGHGECVPGPPDYSCHCNPGYRSHPQHRYCVDVNECEAEPCGPGRG
  ICMNTGGSYNCHCNRGYRLHVGAGGRSCVDLNECAKPHLCGDGGFCINFPGHYKCNCYPGYRLKASRPPV
  CEDIDECRDPSSCPDGKCENKPGSFKCIACQPGYRSQGGGACRDVNECAEGSPCSPGWCENLPGSFRCTC
  AQGYAPAPDGRSCLDVDECEAGDVCDNGICSNTPGSFQCQCLSGYHLSRDRSHCEDIDECDFPAACIGGD
  CINTNGSYRCLCPQGHRLVGGRKCQDIDECSQDPSLCLPHGACKNLQGSYVCVCDEGFTPTQDQHGCEEV
  EQPHHKKECYLNFDDTVFCDSVLATNVTQQECCCSLGAGWGDHCEIYPCPVYSSAEFHSLCPDGKGYTQD
  NNIVNYGIPAHRDIDECMLFGSEICKEGKCVNTQPGYECYCKQGFYYDGNLLECVDVDECLDESNCRNGV
  CENTRGGYRCACTPPAEYSPAQRQCLSPEEMDVDECQDPAACRPGRCVNLPGSYRCECRPPWVPGPSGRD
  CQLPESPAERAPERRDVCWSQRGEDGMCAGPLAGPALTFDDCCCRQGRGWGAQCRPCPPRGAGSHCPTSQ
  SESNSFWDTSPLLLGKPPRDEDSSEEDSDECRCVSGRCVPRPGGAVCECPGGFQLDASRARCVDIDECRE
  LNQRGLLCKSERCVNTSGSFRCVCKAGFARSRPHGACVPQRRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130144
• ORF Size: 3909 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130144.3
• RefSeq ORF: 3912 bp
• Locus ID: 4054
• UniProt ID: Q9NS15
• Cytogenetics: 11q13.1
• Protein Families: Transmembrane
• MW: 139.36 kDa
• Gene Summary: The protein encoded by this gene forms a complex with transforming growth factor beta (TGF-beta) proteins and may be involved in their subcellular localization. Activation of this complex requires removal of the encoded binding protein. This protein also may play a structural role in the extracellular matrix. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]