PLEKHG4 (NM_001129727) Human Tagged ORF Clone

CAT#: RC226353

PLEKHG4 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (PLEKHG4), transcript variant 2

Product Images

Circular map for RC226353

• 

  Western blot validation of overexpression lysate (Cat# LY427036) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226353 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLEKHG4
• Synonyms: ARHGEF44; PRTPHN1; SCA4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226353 representing NM_001129727
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGGCCCCTGGAGAATGGGGATGAGTCCCCAGACTCTCAGGGCCATGCCACCGACTGGAGATTTG
  CTGTGTGCAGTTTCAGGGATGCCTGGGAAGAGGAGGAACCTGCTTCCCAGATGCACGTTAAGGACCCAGG
  TCCTCCAAGACCACCAGCCGGGGCCACCCAGGATGAGGAGCTACAGGGCAGCCCCTTGTCCAGGAAATTC
  CAGTTACCCCCAGCTGCAGATGAGTCGGGGGATGCCCAGAGGGGCACAGTAGAAAGCTCCTCAGTCCTGT
  CAGAAGGGCCAGGCCCCTCTGGAGTGGAGAGTCTCCTATGCCCCATGTCCTCCCACCTCAGCTTGGCACA
  GGGTGAGAGTGACACCCCAGGGGTAGGGTTGGTAGGGGACCCAGGTCCAAGCAGGGCGATGCCATCTGGC
  TTGAGCCCTGGGGCATTGGACAGCGACCCTGTGGGCCTTGGAGACCCTTTATCAGAAATATCAAAGCTGC
  TGGAGGCAGCCCCCAGTGGATCCGGCCTCCCTAAGCCTGCTGACTGCCTCCTGGCCCAAGACCTCTGTTG
  GGAGCTGCTGGCCAGTGGTATGGCCACCTTGCCAGGGACTCGGGATGTCCAAGGCCGGGCAGTGCTGCTT
  CTGTGTGCCCACAGCCCAGCCTGGCTTCAGTCTGAGTGCAGCAGCCAGGAACTCATCCGCCTCCTGCTGT
  ACCTGCGAAGCATCCCCAGGCCCGAAGTACAGGCACTGGGACTGACAGTGCTAGTTGATGCCCGAATTTG
  TGCTCCAAGTTCTTCCCTCTTCTCTGGGCTCAGCCAACTACAAGAAGCAGCCCCAGGGGCCGTGTACCAG
  GTGCTGCTAGTGGGAAGCACGCTGCTGAAGGAAGTGCCTTCCGGGCTGCAGCTGGAGCAGTTGCCTTCTC
  AGAGCCTGCTGACCCACATCCCAACGGCGGGGCTGCCCACTTCGCTAGGAGGAGGCCTGCCTTACTGCCA
  CCAGGCCTGGCTGGATTTCCGAAGGCGGCTGGAAGCTCTACTACAGAACTGCCAGGCAGCTTGTGCCCTG
  CTCCAGGGGGCCATCGAAAGTGTGAAGGCTGTGCCCCAGCCCATGGAGCCTGGGGAGGTCGGTCAGCTGC
  TACAGCAGACAGAGGTCCTGATGCAGCAGGTGCTAGACTCGCCATGGCTGGCATGGCTACAATGCCAGGG
  GGGCCGGGAGCTGACATGGCTGAAGCAAGAGGTCCCAGAGGTGACCCTGAGCCCAGACTACAGGACGGCA
  ATGGACAAGGCTGACGAGCTATATGACCGGGTGGATGGATTGCTGCACCAACTGACCCTGCAGAGCAACC
  AGCGAATACAGGCCCTAGAGTTGGTCCAAACACTGGAGGCCCGGGAAAGCGGACTGCACCAGATTGAAGT
  GTGGCTGCAGCAGGTGGGCTGGCCAGCACTGGAGGAGGCTGGGGAGCCCTCGCTGGACATGCTGCTCCAG
  GCCCAAGGCTCTTTTCAGGAGCTGTACCAGGTTGCCCAGGAGCAGGTCAGGCAAGGGGAGAAGTTTCTGC
  AGCCGCTGACTGGCTGGGAGGCGGCTGAACTGGACCCCCCTGGGGCACGCTTTCTGGCCCTGCGAGCCCA
  GCTGACTGAATTCTCTAGGGCTTTGGCCCAGCGGTGCCAGCGGCTGGCGGATGCTGAGAGGCTGTTTCAG
  CTCTTCAGGGAGGCCTTGACGTGGGCTGAGGAGGGGCAGCGAGTGTTGGCAGAGCTGGAGCAGGAACGCC
  CGGGGGTTGTGTTGCAGCAGCTGCAGCTGCACTGGACCAGGCACCCTGACTTGCCTCCTGCCCACTTCCG
  AAAGATGTGGGCTCTGGCCACGGGGCTGGGCTCAGAGGCCATCCGCCAGGAGTGCCGCTGGGCCTGGGCG
  CGGTGCCAGGACACCTGGCTGGCCCTGGACCAAAAGCTTGAGGCTTCACTGAAGCTACCACCGGTGGGCA
  GCACAGCTAGCCTGTGTGTCAGCCAGGTCCCCGCTGCACCTGCCCACCCTCCCCTGAGGAAGGCCTACAG
  CTTCGATCGGAATCTGGGGCAGAGTCTCAGTGAACCTGCCTGCCACTGCCACCATGCGGCCACTATTGCT
  GCCTGCCGCAGACCAGAGGCTGGAGGAGGTGCCCTGCCCCAGGCATCCCCTACTGTGCCTCCACCAGGCA
  GCTCTGACCCCAGGAGCCTCAACAGGCTACAGCTGGTGCTGGCAGAGATGGTGGCCACGGAGCGGGAGTA
  TGTCCGGGCTCTAGAGTACACTATGGAGAACTATTTCCCCGAGCTGGATCGCCCCGATGTGCCCCAGGGC
  CTCCGCGGTCAGCGTGCCCACCTCTTTGGCAACCTGGAGAAGCTGCGGGACTTCCACTGCCACTTCTTCC
  TGCGTGAGCTGGAGGCTTGCACCCGGCACCCACCACGAGTGGCCTATGCCTTCCTGCGCCATAGGGTGCA
  GTTTGGGATGTACGCGCTCTACAGCAAGAATAAGCCTCGCTCCGATGCCCTGATGTCAAGCTATGGGCAC
  ACCTTCTTCAAGGACAAGCAGCAAGCACTGGGGGACCACCTGGACCTGGCCTCCTACCTGCTAAAGCCCA
  TCCAGCGCATGGGCAAGTACGCACTGCTGCTGCAGGAGCTGGCACGGGCCTGCGGGGGCCCCACGCAGGA
  GCTCAGTGCGCTGCGGGAGGCCCAGAGCCTTGTGCACTTCCAGCTGCGGCACGGAAACGACCTGCTGGCC
  ATGGACGCCATCCAGGGCTGTGATGTTAACCTCAAGGAACAGGGGCAGCTGGTGCGACAGGATGAGTTTG
  TGGTGCGCACTGGGCGCCACAAGTCCGTGCGCCGCATCTTCCTTTTTGAGGAGCTGCTGCTCTTCAGCAA
  GCCTCGCCATGGGCCCACAGGGGTTGACACATTTGCCTACAAGCGCTCCTTCAAGATGGCAGACCTTGGT
  CTCACTGAGTGCTGTGGGAACAGCAACCTGCGCTTCGAGATCTGGTTCCGCCGCCGCAAGGCCAGGGACA
  CCTTTGTGCTGCAGGCCTCCAGCCTGGCTATCAAGCAGGCCTGGACAGCTGACATCTCCCACCTGCTTTG
  GAGGCAGGCCGTCCACAACAAGGAGGTGCGCATGGCTGAGATGGTGTCCATGGGTGTGGGGAACAAGGCC
  TTCCGAGACATTGCTCCCAGCGAGGAAGCCATCAACGACCGCACCGTCAACTATGTCCTGAAGTGCCGAG
  AAGTTCGCTCTCGGGCGTCCATTGCCGTAGCCCCGTTTGACCATGACAGCCTCTACCTGGGGGCCTCGAA
  CTCCCTTCCTGGAGACCCTGCCTCTTGCTCTGTTCTGGGGTCCCTCAACCTGCACCTGTACAGAGACCCA
  GCTCTTCTGGGTCTCCGCTGTCCCCTGTATCCCAGCTTCCCAGAGGAAGCAGCACTGGAGGCTGAGGCAG
  AGCTGGGCGGCCAGCCCTCTTTGACTGCTGAGGACTCAGAGATCTCGTCCCAATGCCCATCAGCCAGTGG
  CTCCAGTGGCTCTGACAGCAGCTGTGTGTCAGGGCAGGCCCTGGGTAGGGGCCTGGAGGACTTACCCTGT
  GTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226353 representing NM_001129727
  Red=Cloning site Green=Tags(s)
  
  MERPLENGDESPDSQGHATDWRFAVCSFRDAWEEEEPASQMHVKDPGPPRPPAGATQDEELQGSPLSRKF
  QLPPAADESGDAQRGTVESSSVLSEGPGPSGVESLLCPMSSHLSLAQGESDTPGVGLVGDPGPSRAMPSG
  LSPGALDSDPVGLGDPLSEISKLLEAAPSGSGLPKPADCLLAQDLCWELLASGMATLPGTRDVQGRAVLL
  LCAHSPAWLQSECSSQELIRLLLYLRSIPRPEVQALGLTVLVDARICAPSSSLFSGLSQLQEAAPGAVYQ
  VLLVGSTLLKEVPSGLQLEQLPSQSLLTHIPTAGLPTSLGGGLPYCHQAWLDFRRRLEALLQNCQAACAL
  LQGAIESVKAVPQPMEPGEVGQLLQQTEVLMQQVLDSPWLAWLQCQGGRELTWLKQEVPEVTLSPDYRTA
  MDKADELYDRVDGLLHQLTLQSNQRIQALELVQTLEARESGLHQIEVWLQQVGWPALEEAGEPSLDMLLQ
  AQGSFQELYQVAQEQVRQGEKFLQPLTGWEAAELDPPGARFLALRAQLTEFSRALAQRCQRLADAERLFQ
  LFREALTWAEEGQRVLAELEQERPGVVLQQLQLHWTRHPDLPPAHFRKMWALATGLGSEAIRQECRWAWA
  RCQDTWLALDQKLEASLKLPPVGSTASLCVSQVPAAPAHPPLRKAYSFDRNLGQSLSEPACHCHHAATIA
  ACRRPEAGGGALPQASPTVPPPGSSDPRSLNRLQLVLAEMVATEREYVRALEYTMENYFPELDRPDVPQG
  LRGQRAHLFGNLEKLRDFHCHFFLRELEACTRHPPRVAYAFLRHRVQFGMYALYSKNKPRSDALMSSYGH
  TFFKDKQQALGDHLDLASYLLKPIQRMGKYALLLQELARACGGPTQELSALREAQSLVHFQLRHGNDLLA
  MDAIQGCDVNLKEQGQLVRQDEFVVRTGRHKSVRRIFLFEELLLFSKPRHGPTGVDTFAYKRSFKMADLG
  LTECCGNSNLRFEIWFRRRKARDTFVLQASSLAIKQAWTADISHLLWRQAVHNKEVRMAEMVSMGVGNKA
  FRDIAPSEEAINDRTVNYVLKCREVRSRASIAVAPFDHDSLYLGASNSLPGDPASCSVLGSLNLHLYRDP
  ALLGLRCPLYPSFPEEAALEAEAELGGQPSLTAEDSEISSQCPSASGSSGSDSSCVSGQALGRGLEDLPC
  V
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001129727
• ORF Size: 3573 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001129727.3
• RefSeq Size: 4606 bp
• RefSeq ORF: 3576 bp
• Locus ID: 25894
• UniProt ID: Q58EX7
• Cytogenetics: 16q22.1
• MW: 130.8 kDa
• Gene Summary: The protein encoded by this gene can function as a guanine nucleotide exchange factor (GEF) and may play a role in intracellular signaling and cytoskeleton dynamics at the Golgi apparatus. Polymorphisms in the region of this gene have been found to be associated with spinocerebellar ataxia in some study populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]