FAM123C (AMER3) (NM_001105193) Human Tagged ORF Clone

CAT#: RC226219

AMER3 (Myc-DDK-tagged)-Human family with sequence similarity 123C (FAM123C), transcript variant 2

Product Images

Circular map for RC226219

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM123C
• Synonyms: FAM123C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226219 representing NM_001105193
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGAAGAGAGGAAAGACCTTCATCAAGTCCAGCCTGCAGGTTTCCCACGAGAAACCCCCAGACC
  CAGCAGCCGTGGCTGCAGCCAGGGAGGGGACAGGCCCCTGGTCAGTCCTTCCAGGAGGGCAACAGAGGCC
  CCACAGTGAGAAGGGCCCCCAAGCCAGCCCCAGTGCCCAAGAATACGACAGATGCCCCAACAAAGGGGCG
  CAGCTGGACCCCAAAGGGGGACCCGCAGCCCTCTGTGGAGCCACCTTCAAACCGGTGCGAAAGTGCAAGA
  CTCACGACAGCATGTCTGGGGCAGGCAGGGCCACGGCTGCCACAGGGCAGCTGGTGGGCAGTGCAAGCTT
  CCCGGGCTCCCCGGGCAGCCGGCGCATGATCGACTACCGCCACTTTGTGCCCCAGATGCCCTTTGTGCCA
  GCTGTGGCCAAGAGCATCCCGAGGAAGAGGATTTCCCTGAAGAGGCCCAAGAAGTGCTTTCGGAACCTAT
  TCCACATTCGGAGAAACAAGACTGAGGACTTGGCCTCGCTGGCGGCCGAGGGGAAAAGCCTGCCCTCCCC
  AGGGGACCCGTCAGACCCTGGGGGGCGGCGAAGCAAAGCCTTCCTCCCCCCGGGTGAGGGGCCGGGGCTG
  GACGGCCTGTGCCAGGACCTGTTGGACAGCGAGCTCCTGGCCGATGCATCCTTTGGTCTCTGCAGGGCCC
  TGTGTGAGGACGTGGCCTCACTCCAGAGCTTCGACTCGCTCACGGGTTGTGGGGAGGTGTTCGCAGATGA
  GAGCTCGGTGCCATCTCTGGAGCTGAACGAGGGCCCGGAGAGCCCAACCCAGGCTGCTCAGGGCCTGGAG
  AGCAAGGTTCCCAGGGGCCCTCTCCAGGGCAGTGTGGAGCAGCTGGCCTCGCCCGCCCAGAATGAAGCCT
  CTGACTTCACCAGGTTCTGGGACAGTGTGAATCGCTCAGTGCGTCAGCAGCAGCGTGCCCTCCTAGGCCC
  GTGGCTTTCAGGCCCCCAGGGGACAGACAGGGACCAACCCCGGCTGGACACAGCTGGGCTCGCTGAGCTG
  CCCCTCTGCCCCTGCAGGGACCCTCGCAGCGGCTCCAAAGCCAGCTCCATCGACACAGGCACCCCCAAGA
  GCGAGCAGCCCGAATCCGTGTCCACAAGTGACGAGGGCTACTATGATTCCTTCTCGCCAGGACTTGAGGA
  GGACAAGAAGGAAGCTGAGAGCCCAGGCACTCCTGCCGCCACCTTCCCACGGGACAGCTACAGTGGGGAC
  GCCCTCTACGAGCTCTTCCACGACCCCAGCGAGGGTCCTCTTGGCCCCAGCCCAGATGATGACCTGTGCG
  TGTCTGAGAGTCTGTCAGGGCCGGCCCTGGGGACGCCACTGTCCATATGCAGCTTCCGAGTGGGGGCCGA
  GGAGAACTTGGCCCCAGCACCAGGCCCTGACCTGCTCAGCCAGGGCTTCCTACAGAGCTCCTGGAAGGGC
  AAGGAGTGCCTGCTGAAGCTGTGTGACACTGAGCTCGCCATCACCATGGGCATCGTCAGCTGGCTGCGCC
  GAGGCCCCACGCCCCGTGCCCCACCCACCCCTGGGCAGCCTGCAGCTCCACCTGGTTCCCAGGGAGCCCC
  TAGGGCACCCACAGAGAAGCTGGGGGGCAGGGAGGGCCTGGCCTCAGATGCAGGGGGGGCGACAGTTTGC
  TCAGCACCCAGCAGGCAGGAGCTGTGGGCACACCCGGGCACCACAGGCCTGCTCGCCGGAGAGAGCAAGG
  CCCTCGGAGGGGCCACACAAGGGACTGGCACACTGTCCAGGGATGCCTCTCGAGAGGAAGAGACACGAGG
  TCACTCTGAAGGCTTGTTCTCCTCTATGGAGTCTGCAGCCACTTCGACAACAGATACTTCCGGTAAAAAT
  AAGGCCCCAGTTCCTTCTACCTGGCCCTGCTCCCAGAAGGAGCCTGGGCCACCAGGGGTCCTGGGGTGTT
  TCCGAGGCCCCTGGAGGCCAGGTCACGGAGGTGACACTCTGGATGCAGAGCCCATGCTGGCAGGCTGTGT
  GGCCCGTGTGGCAGCCCTGAAGATCAGCTCAAACGAACAGCCCCCGGCCGCATGGCCTCCAAGGCAAGAC
  ATGGGCAGTGGGCTCTTTGGGCAGCGCTGGGCCAGGGGCCCTGACATGCTGGAGCAGAAACAGTCCAGCA
  GCTCCCCCAGCATGACCACCATCCATGGCCTACCCTACTCAGCCAGCACACAGGACCAGAGGTGTCGAGA
  TCGTGTCCAGGACCTGAGCTGGCTCAGGGTGGAGCCCACCGGGCTAGGTGTCCAGGCCTGGGCCTCTGTG
  GAGGACCAGCCCTTGCAGCTCAGCACAGAGGCTGTGGAGCAGGTGGCACACGGCAGCCAGCTGGACTCTG
  AGCCCCGCTCAGCCCCTGCTGCCCGGTGGAGTTCCCAGGGCCACCATCCAGAAAGCCTGGGCCTCACTTT
  GAACAGCCAGCAGGAAGGGGGGGTCTCTGCAAGTGCCCCAGAATGCCGCTGCAGCCTCCTGGCCCGTGAG
  GGCCTCCTCTGTGGCCAGCCAGAAGTGGGGGCCTCTGGGCCAGCCATGGCTGAGCCCCATCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226219 representing NM_001105193
  Red=Cloning site Green=Tags(s)
  
  MELKRGKTFIKSSLQVSHEKPPDPAAVAAAREGTGPWSVLPGGQQRPHSEKGPQASPSAQEYDRCPNKGA
  QLDPKGGPAALCGATFKPVRKCKTHDSMSGAGRATAATGQLVGSASFPGSPGSRRMIDYRHFVPQMPFVP
  AVAKSIPRKRISLKRPKKCFRNLFHIRRNKTEDLASLAAEGKSLPSPGDPSDPGGRRSKAFLPPGEGPGL
  DGLCQDLLDSELLADASFGLCRALCEDVASLQSFDSLTGCGEVFADESSVPSLELNEGPESPTQAAQGLE
  SKVPRGPLQGSVEQLASPAQNEASDFTRFWDSVNRSVRQQQRALLGPWLSGPQGTDRDQPRLDTAGLAEL
  PLCPCRDPRSGSKASSIDTGTPKSEQPESVSTSDEGYYDSFSPGLEEDKKEAESPGTPAATFPRDSYSGD
  ALYELFHDPSEGPLGPSPDDDLCVSESLSGPALGTPLSICSFRVGAEENLAPAPGPDLLSQGFLQSSWKG
  KECLLKLCDTELAITMGIVSWLRRGPTPRAPPTPGQPAAPPGSQGAPRAPTEKLGGREGLASDAGGATVC
  SAPSRQELWAHPGTTGLLAGESKALGGATQGTGTLSRDASREEETRGHSEGLFSSMESAATSTTDTSGKN
  KAPVPSTWPCSQKEPGPPGVLGCFRGPWRPGHGGDTLDAEPMLAGCVARVAALKISSNEQPPAAWPPRQD
  MGSGLFGQRWARGPDMLEQKQSSSSPSMTTIHGLPYSASTQDQRCRDRVQDLSWLRVEPTGLGVQAWASV
  EDQPLQLSTEAVEQVAHGSQLDSEPRSAPAARWSSQGHHPESLGLTLNSQQEGGVSASAPECRCSLLARE
  GLLCGQPEVGASGPAMAEPHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105193
• ORF Size: 2583 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105193.1, NP_001098663.1
• RefSeq ORF: 2586 bp
• Locus ID: 205147
• UniProt ID: Q8N944
• Cytogenetics: 2q21.1
• MW: 90.3 kDa
• Gene Summary: Regulator of the canonical Wnt signaling pathway. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane (By similarity).[UniProtKB/Swiss-Prot Function]