CDC27 (NM_001114091) Human Tagged ORF Clone

CAT#: RC226198

CDC27 (Myc-DDK-tagged)-Human cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 1

Product Images

Circular map for RC226198

• 

  Western blot validation of overexpression lysate (Cat# LY426445) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226198 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDC27
• Synonyms: ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E; H-NUC; HNUC; NUC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226198 representing NM_001114091
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGTGCTGCAGGAACCCGTCCAGGCTGCTATATGGCAAGCACTAAACCACTATGCTTACCGAGATG
  CGGTTTTCCTCGCAGAACGCCTTTATGCAGAAGTACACTCAGAAGAAGCCTTGTTTTTACTGGCAACCTG
  TTATTACCGCTCAGGAAAGGCATATAAAGCATATAGACTCTTGAAAGGACACAGTTGTACTACACCGCAA
  TGCAAATACCTGCTTGCAAAATGTTGTGTTGATCTCAGCAAGCTTGCAGAAGGGGAACAAATCTTATCTG
  GTGGAGTGTTTAATAAGCAGAAAAGCCATGATGATATTGTTACTGAGTTTGGTGATTCAGCTTGCTTTAC
  TCTTTCATTGTTGGGACATGTATATTGCAAGACAGATCGGCTTGCCAAAGGATCAGAATGTTACCAAAAG
  AGCCTTAGTTTAAATCCTTTCCTCTGGTCTCCCTTTGAATCATTATGTGAAATAGGTGAAAAGCCAGATC
  CTGACCAAACATTTAAATTCACATCTTTACAGAACTTTAGCAACTGTCTGCCCAACTCTTGCACAACACA
  AGTACCTAATCATAGTTTATCTCACAGACAGCCTGAGACAGTTCTTACGGAAACACCCCAGGACACAATT
  GAATTAAACAGATTGAATTTAGAATCTTCCAATTCAAAGTACTCCTTGAATACAGATTCCTCAGTGTCTT
  ATATTGATTCAGCTGTAATTTCACCTGATACTGTCCCACTGGGAACAGGAACTTCCATATTATCTAAACA
  GGTTCAAAATAAACCAAAAACTGGTCGAAGTTTATTAGGAGGACCAGCAGCTCTTAGTCCATTAACCCCA
  AGTTTTGGGATTTTGCCATTAGAAACCCCAAGTCCTGGAGATGGATCCTATTTACAAAACTACACTAATA
  CACCTCCTGTAATTGATGTGCCATCCACCGGAGCCCCTTCAAAAAAGACTTTTCGTGTTTTACAGTCTGT
  TGCCAGAATCGGCCAAACTGGAACAAAGTCTGTCTTCTCACAGAGTGGAAATAGCCGAGAGGTAACTCCA
  ATTCTTGCACAAACACAAAGTTCTGGTCCACAAACAAGTACAACACCTCAGGTATTGAGCCCCACTATTA
  CATCTCCCCCAAACGCACTGCCTCGAAGAAGTTCACGACTCTTTACTAGTGACAGCTCCACAACCAAGGA
  GAATAGCAAAAAATTAAAAATGAAGTTTCCACCTAAAATCCCAAACAGAAAAACAAAAAGTAAAACTAAT
  AAAGGAGGAATAACTCAACCTAACATAAATGATAGCCTGGAAATTACAAAATTGGACTCTTCCATCATTT
  CAGAAGGGAAAATATCCACAATCACACCTCAGATTCAGGCCTTTAATCTACAAAAAGCAGCAGCAGAAGG
  TTTGATGAGCCTTCTTCGTGAAATGGGGAAAGGTTATTTAGCTTTGTGTTCATACAACTGCAAAGAAGCT
  ATAAATATTTTGAGCCATCTACCTTCTCACCACTACAATACTGGTTGGGTACTGTGCCAAATTGGAAGGG
  CCTATTTTGAACTTTCAGAGTACATGCAAGCTGAAAGAATATTCTCAGAGGTTAGAAGGATTGAGAATTA
  TAGAGTTGAAGGCATGGAGATCTACTCTACAACACTTTGGCATCTTCAAAAAGATGTTGCTCTTTCAGTT
  CTGTCAAAAGACTTAACAGACATGGATAAAAATTCGCCAGAGGCCTGGTGTGCTGCAGGGAACTGTTTCA
  GTCTGCAACGGGAACATGATATTGCAATTAAATTCTTCCAGAGAGCTATCCAAGTTGATCCAAATTACGC
  TTATGCCTATACTCTATTAGGGCATGAGTTTGTCTTAACTGAAGAATTGGACAAAGCATTAGCTTGTTTT
  CGAAATGCTATCAGAGTCAATCCTAGACATTATAATGCATGGTATGGTTTAGGAATGATTTATTACAAGC
  AAGAAAAATTCAGCCTTGCAGAAATGCATTTCCAAAAAGCGCTTGATATCAACCCTCAAAGTTCAGTTTT
  ACTTTGCCACATTGGAGTAGTTCAACATGCACTGAAAAAATCAGAGAAGGCTTTGGATACCCTAAACAAA
  GCCATTGTCATTGATCCCAAGAACCCTCTATGCAAATTTCACAGAGCCTCAGTTTTATTTGCAAATGAAA
  AATATAAGTCTGCTTTACAAGAACTTGAAGAATTGAAACAAATTGTTCCCAAAGAATCCCTCGTTTACTT
  CTTAATAGGAAAGGTTTACAAGAAGTTAGGTCAAACGCACCTCGCCCTGATGAATTTCTCTTGGGCTATG
  GATTTAGATCCTAAAGGAGCCAATAACCAGATTAAAGAGGCAATTGATAAGCGTTATCTTCCAGATGATG
  AGGAGCCAATAACCCAAGAAGAACAGATCATGGGAACAGATGAATCCCAGGAGAGCAGCATGACAGATGC
  GGATGACACACAACTTCATGCAGCTGAAAGTGATGAATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226198 representing NM_001114091
  Red=Cloning site Green=Tags(s)
  
  MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQ
  CKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQK
  SLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTI
  ELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTP
  SFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTP
  ILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTN
  KGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEA
  INILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSV
  LSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACF
  RNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNK
  AIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAM
  DLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001114091
• ORF Size: 2490 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114091.4
• RefSeq ORF: 2493 bp
• Locus ID: 996
• UniProt ID: P30260
• Cytogenetics: 17q21.32
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• MW: 92.4 kDa
• Gene Summary: The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014]