CDC27 (NM_001114091) Human Untagged Clone

CAT#: SC318990

CDC27 (untagged)-Human cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC27
• Synonyms: ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E; H-NUC; HNUC; NUC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001114091 edited
  CCCCGGTAACGGTCGCTGGTGAGTTTAAATGAGCAGGGGCTGGCCGGGCCGGAGCCGCTA
  CAGGGGGGGCCTGAGGCACTGCAGAAAGTGGGCCTGAGCCTCGAGGATGACGGTGCTGCA
  GGAACCCGTCCAGGCTGCTATATGGCAAGCACTAAACCACTATGCTTACCGAGATGCGGT
  TTTCCTCGCAGAACGCCTTTATGCAGAAGTACACTCAGAAGAAGCCTTGTTTTTACTGGC
  AACCTGTTATTACCGCTCAGGAAAGGCATATAAAGCATATAGACTCTTGAAAGGACACAG
  TTGTACTACACCGCAATGCAAATACCTGCTTGCAAAATGTTGTGTTGATCTCAGCAAGCT
  TGCAGAAGGGGAACAAATCTTATCTGGTGGAGTGTTTAATAAGCAGAAAAGCCATGATGA
  TATTGTTACTGAGTTTGGTGATTCAGCTTGCTTTACTCTTTCATTGTTGGGACATGTATA
  TTGCAAGACAGATCGGCTTGCCAAAGGATCAGAATGTTACCAAAAGAGCCTTAGTTTAAA
  TCCTTTCCTCTGGTCTCCCTTTGAATCATTATGTGAAATAGGTGAAAAGCCAGATCCTGA
  CCAAACATTTAAATTCACATCTTTACAGAACTTTAGCAACTGTCTGCCCAACTCTTGCAC
  AACACAAGTACCTAATCATAGTTTATCTCACAGACAGCCTGAGACAGTTCTTACGGAAAC
  ACCCCAGGACACAATTGAATTAAACAGATTGAATTTAGAATCTTCCAATTCAAAGTACTC
  CTTGAATACAGATTCCTCAGTGTCTTATATTGATTCAGCTGTAATTTCACCTGATACTGT
  CCCACTGGGAACAGGAACTTCCATATTATCTAAACAGGTTCAAAATAAACCAAAAACTGG
  TCGAAGTTTATTAGGAGGACCAGCAGCTCTTAGTCCATTAACCCCAAGTTTTGGGATTTT
  GCCATTAGAAACCCCAAGTCCTGGAGATGGATCCTATTTACAAAACTACACTAATACACC
  TCCTGTAATTGATGTGCCATCCACCGGAGCCCCTTCAAAAAAGACTTTTCGTGTTTTACA
  GTCTGTTGCCAGAATCGGCCAAACTGGAACAAAGTCTGTCTTCTCACAGAGTGGAAATAG
  CCGAGAGGTAACTCCAATTCTTGCACAAACACAAAGTTCTGGTCCACAAACAAGTACAAC
  ACCTCAGGTATTGAGCCCCACTATTACATCTCCCCCAAACGCACTGCCTCGAAGAAGTTC
  ACGACTCTTTACTAGTGACAGCTCCACAACCAAGGAGAATAGCAAAAAATTAAAAATGAA
  GTTTCCACCTAAAATCCCAAACAGAAAAACAAAAAGTAAAACTAATAAAGGAGGAATAAC
  TCAACCTAACATAAATGATAGCCTGGAAATTACAAAATTGGACTCTTCCATCATTTCAGA
  AGGGAAAATATCCACAATCACACCTCAGATTCAGGCCTTTAATCTACAAAAAGCAGCAGC
  AGAAGGTTTGATGAGCCTTCTTCGTGAAATGGGGAAAGGTTATTTAGCTTTGTGTTCATA
  CAACTGCAAAGAAGCTATAAATATTTTGAGCCATCTACCTTCTCACCACTACAATACTGG
  TTGGGTACTGTGCCAAATTGGAAGGGCCTATTTTGAACTTTCAGAGTACATGCAAGCTGA
  AAGAATATTCTCAGAGGTTAGAAGGATTGAGAATTATAGAGTTGAAGGCATGGAGATCTA
  CTCTACAACACTTTGGCATCTTCAAAAAGATGTTGCTCTTTCAGTTCTGTCAAAAGACTT
  AACAGACATGGATAAAAATTCGCCAGAGGCCTGGTGTGCTGCAGGGAACTGTTTCAGTCT
  GCAACGGGAACATGATATTGCAATTAAATTCTTCCAGAGAGCTATCCAAGTTGATCCAAA
  TTACGCTTATGCCTATACTCTATTAGGGCATGAGTTTGTCTTAACTGAAGAATTGGACAA
  AGCATTAGCTTGTTTTCGAAATGCTATCAGAGTCAATCCTAGACATTATAATGCATGGTA
  TGGTTTAGGAATGATTTATTACAAGCAAGAAAAATTCAGCCTTGCAGAAATGCATTTCCA
  AAAAGCGCTTGATATCAACCCTCAAAGTTCAGTTTTACTTTGCCACATTGGAGTAGTTCA
  ACATGCACTGAAAAAATCAGAGAAGGCTTTGGATACCCTAAACAAAGCCATTGTCATTGA
  TCCCAAGAACCCTCTATGCAAATTTCACAGAGCCTCAGTTTTATTTGCAAATGAAAAATA
  TAAGTCTGCTTTACAAGAACTTGAAGAATTGAAACAAATTGTTCCCAAAGAATCCCTCGT
  TTACTTCTTAATAGGAAAGGTTTACAAGAAGTTAGGTCAAACGCACCTCGCCCTGATGAA
  TTTCTCTTGGGCTATGGATTTAGATCCTAAAGGAGCCAATAACCAGATTAAAGAGGCAAT
  TGATAAGCGTTATCTTCCAGATGATGAGGAGCCAATAACCCAAGAAGAACAGATCATGGG
  AACAGATGAATCCCAGGAGAGCAGCATGACAGATGCGGATGACACACAACTTCATGCAGC
  TGAAAGTGATGAATTTTAACTTCTGGAAATCAGACTTTTACAACTGGATGTGTGACTAGT
  GCTGACGTGTTTCTTGTCCCTCTGTATACTGAGTCTTTACTCTTGAGCTGGCGGTGTCAT
  CGTCCGTCACTTATACCATGAGTGTGCCACTTTCATTGGACCCTGACTGTATACAGAATG
  AAAGGCAGTGCAATATTTAGCTGCTAACAAGACTGGCTCTTTTACCAGTATGAATGACAA
  TTTATGGGGGGTAGGGTGGGGAACTTTCTTTTCTGTTTTTCTTTAATCTCCCTTTGTTGG
  AAAGTATCATGAAAGGAAGAGTTATGCTTTATCTTGAAGGAACCATTAGATATGGAAAAT
  AGTGATGAACCAGAGTTTCTTGGTTGCTTTTTCAAAAATTTGTTTTTATTTGGTTCTGTT
  CCTGATAAACAGAGTAACTGACCTTCATTTCTAGGTTCTTCAAGAATGGTGTTTGCAAGT
  GCCAGATGGAACAATAAAAGACGTTGCCTATAACAGTGAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001114091
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001114091.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114091.1, NP_001107563.1
• RefSeq Size: 5611 bp
• RefSeq ORF: 2493 bp
• Locus ID: 996
• UniProt ID: P30260
• Cytogenetics: 17q21.32
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• Gene Summary: The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.