BANK1 (NM_001127507) Human Tagged ORF Clone

CAT#: RC226064

BANK1 (Myc-DDK-tagged)-Human B-cell scaffold protein with ankyrin repeats 1 (BANK1), transcript variant 3

Product Images

Circular map for RC226064

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BANK1
• Synonyms: BANK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226064 representing NM_001127507
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCAGCAGCGCCAGGCAAGGGGCTTGGGAGCCCGGACCCCGCCCCCTGCGGCCCAGCGCCCCCAG
  ATTCTGAAGACTACTTTGAGGTCAACATTCCAACAGACCTACGAGCAAAACATTCTGGGGAAATAAGTGA
  GAGAAAGGAAATTGAAGAACTATCAGAAGCTTCAAGAAACACCATACCACTAGCAGTGGTGCTTCCCACT
  GAAATTCCATGTGAGAATCCTGGTGAAATATTCATAATTTTGAGAGATGAAGTAATTGGTGATACTGTAG
  AGGTTGAATTTACATCAAGTAATAAGCGCATTAGAACACGGCCAGCCCTTTGGAATAAGAAAGTCTGGTG
  CATGAAAGCTTTAGAGTTTCCTGCTGGTTCAGTCCATGTCAATGTCTACTGTGATGGAATCGTTAAAGCT
  ACAACCAAAATTAAGTACTACCCAACAGCAAAGGCAAAGGAATGCCTATTCAGAATGGCAGATTCAGGAG
  AGAGTTTGTGCCAGAATAGCATTGAAGAACTTGATGGTGTCCTTACATCCATATTCAAACATGAGATACC
  ATATTATGAGTTCCAGTCTCTTCAAACTGAAATTTGTTCTCAAAACAAATATACTCATTTCAAAGAACTT
  CCAACTCTTCTCCACTGTGCAGCAAAATTTGGCTTAAAGAACCTGGCTATTCATTTGCTTCAATGTTCAG
  GAGCAACCTGGGCATCTAAGATGAAAAATATGGAGGGTTCAGACCCCGCACATATTGCTGAAAGGCATGG
  TCACAAAGAACTCAAGAAAATCTTCGAAGACTTTTCAATCCAAGAAATTGACATAAATAATGAGCAAGAA
  AATGATTATGAAGAGGATATTGCCTCATTTTCCACATATATTCCTTCCACACAGAACCCAGCATTTCATC
  ATGAAAGCAGGAAGACATACGGGCAGAGTGCAGATGGAGCTGAGGCAAATGAAATGGAAGGGGAAGGAAA
  ACAGAATGGATCAGGCATGGAGACCAAACACAGCCCACTAGAGGTTGGCAGTGAGAGTTCTGAAGACCAG
  TATGATGACTTGTATGTGTTCATTCCTGGTGCTGATCCAGAAAATAATTCACAAGAGCCACTCATGAGCA
  GCAGACCTCCTCTCCCCCCGCCGCGACCTGTAGCTAATGCCTTCCAACTGGAAAGACCTCACTTCACCTT
  ACCAGGGACAATGGTGGAAGGCCAAATGGAAAGAAGTCAAAACTGGGGTCATCCTGGTGTTAGACAAGAA
  ACAGGAGATGAACCCAAAGGAGAAAAAGAGAAGAAAGAAGAGGAAAAAGAGCAGGAGGAGGAAGAAGACC
  CATATACTTTTGCTGAGATTGATGACAGTGAATATGACATGATATTGGCCAATCTGAGTATAAAGAAAAA
  AACTGGGAGTCGGTCTTTCATTATAAATAGACCTCCTGCCCCCACACCCCGACCCACAAGTATACCTCCA
  AAAGAGGAAACTACACCTTACATAGCTCAAGTGTTTCAACAAAAGACAGCCAGAAGACAATCTGATGATG
  ACAAGTTCCGTGGTCTTCCTAAGAAACAAGACAGAGCTCGGATAGAGAGTCCAGCCTTTTCTACTCTCAG
  GGGCTGTCTAACTGATGGTCAGGAAGAACTCATCCTCCTGCAGGAGAAAGTAAAGAATGGGAAAATGTCT
  ATGGATGAAGCTCTGGAGAAATTTAAACACTGGCAGATGGGAAAAAGTGGCCTGGAAATGATTCAGCAGG
  AGAAATTACGACAACTACGAGACTGCATTATTGGGAAAAGGCCAGAAGAAGAAAATGTCTATAATAAACT
  CACCATTGTGCACCATCCAGGTGGTAAGGAAACTGCCCACAATGAAAATAAGTTTTATAATGTACACTTC
  AGCAATAAGCTTCCTGCTCGACCCCAAGTTGAAAAGGAATTTGGTTTCTGTTGCAAGAAAGATCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226064 representing NM_001127507
  Red=Cloning site Green=Tags(s)
  
  MLPAAPGKGLGSPDPAPCGPAPPDSEDYFEVNIPTDLRAKHSGEISERKEIEELSEASRNTIPLAVVLPT
  EIPCENPGEIFIILRDEVIGDTVEVEFTSSNKRIRTRPALWNKKVWCMKALEFPAGSVHVNVYCDGIVKA
  TTKIKYYPTAKAKECLFRMADSGESLCQNSIEELDGVLTSIFKHEIPYYEFQSLQTEICSQNKYTHFKEL
  PTLLHCAAKFGLKNLAIHLLQCSGATWASKMKNMEGSDPAHIAERHGHKELKKIFEDFSIQEIDINNEQE
  NDYEEDIASFSTYIPSTQNPAFHHESRKTYGQSADGAEANEMEGEGKQNGSGMETKHSPLEVGSESSEDQ
  YDDLYVFIPGADPENNSQEPLMSSRPPLPPPRPVANAFQLERPHFTLPGTMVEGQMERSQNWGHPGVRQE
  TGDEPKGEKEKKEEEKEQEEEEDPYTFAEIDDSEYDMILANLSIKKKTGSRSFIINRPPAPTPRPTSIPP
  KEETTPYIAQVFQQKTARRQSDDDKFRGLPKKQDRARIESPAFSTLRGCLTDGQEELILLQEKVKNGKMS
  MDEALEKFKHWQMGKSGLEMIQQEKLRQLRDCIIGKRPEEENVYNKLTIVHHPGGKETAHNENKFYNVHF
  SNKLPARPQVEKEFGFCCKKDH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127507
• ORF Size: 1956 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127507.2, NP_001120979.2
• RefSeq ORF: 1959 bp
• Locus ID: 55024
• UniProt ID: Q8NDB2
• Cytogenetics: 4q24
• MW: 73.5 kDa
• Gene Summary: The protein encoded by this gene is a B-cell-specific scaffold protein that functions in B-cell receptor-induced calcium mobilization from intracellular stores. This protein can also promote Lyn-mediated tyrosine phosphorylation of inositol 1,4,5-trisphosphate receptors. Polymorphisms in this gene are associated with susceptibility to systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]