CLK3 (NM_001130028) Human Tagged ORF Clone

CAT#: RC226050

CLK3 (Myc-DDK-tagged)-Human CDC-like kinase 3 (CLK3), transcript variant 1

Product Images

Circular map for RC226050

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CLK3
• Synonyms: PHCLK3; PHCLK3/152
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226050 representing NM_001130028
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGCCCGTCCTCTCCGCGCGCAGGAGGGAGTTGGCGGACCACGCGGGGTCGGGGCGACGGAGCGGGCCCA
  GCCCCACGGCCAGGTCGGGGCCCCACCTCTCGGCTCTGAGAGCCCAGCCGGCCCGGGCCGCGCACCTGTC
  AGGTCGGGGGACCTACGTGCGCCGCGACACGGCGGGAGGCGGGCCGGGCCAGGCTCGTCCCCTCGGCCCT
  CCCGGAACTAGTCTCCTAGGCCGCGGCGCCCGCCGGAGCGGAGAGGGCTGGTGCCCCGGAGCCTTCGAGT
  CGGGGGCTAGAGCGGCCAGGCCTCCGAGCCGGGTCGAGCCGAGGCTGGCGACGGCTGCGTCACGCGAGGG
  GGCGGGGCTGCCACGGGCGGAGGTCGCAGCCGGAAGCGGAAGAGGCGCTCGGAGCGGGGAGTGGGGCCTA
  GCTGCAGCCGGAGCCTGGGAGACGATGCATCACTGTAAGCGATACCGCTCCCCTGAACCAGACCCGTACC
  TGAGCTACCGATGGAAGAGGAGGAGGTCCTACAGTCGGGAACATGAAGGGAGACTGCGATACCCGTCCCG
  AAGGGAGCCTCCCCCACGAAGATCTCGGTCCAGAAGCCATGACCGCCTGCCCTACCAGAGGAGGTACCGG
  GAGCGCCGTGACAGCGATACATACCGGTGTGAAGAGCGGAGCCCATCCTTTGGAGAGGACTACTATGGAC
  CTTCACGTTCTCGTCATCGTCGGCGATCGCGGGAGAGGGGGCCATACCGGACCCGCAAGCATGCCCACCA
  CTGCCACAAACGCCGCACCAGGTCTTGTAGCAGCGCCTCCTCGAGAAGCCAACAGAGCAGTAAGCGCAGC
  AGCCGGAGTGTGGAAGATGACAAGGAGGGTCACCTGGTGTGCCGGATCGGCGATTGGCTCCAAGAGCGAT
  ATGAGATTGTGGGGAACCTGGGTGAAGGCACCTTTGGCAAGGTGGTGGAGTGCTTGGACCATGCCAGAGG
  GAAGTCTCAGGTTGCCCTGAAGATCATCCGCAACGTGGGCAAGTACCGGGAGGCTGCCCGGCTAGAAATC
  AACGTGCTCAAAAAAATCAAGGAGAAGGACAAAGAAAACAAGTTCCTGTGTGTCTTGATGTCTGACTGGT
  TCAACTTCCACGGTCACATGTGCATCGCCTTTGAGCTCCTGGGCAAGAACACCTTTGAGTTCCTGAAGGA
  GAATAACTTCCAGCCTTACCCCCTACCACATGTCCGGCACATGGCCTACCAGCTCTGCCACGCCCTTAGA
  TTTCTGCATGAGAATCAGCTGACCCATACAGACTTGAAACCAGAGAACATCCTGTTTGTGAATTCTGAGT
  TTGAAACCCTCTACAATGAGCACAAGAGCTGTGAGGAGAAGTCAGTGAAGAACACCAGCATCCGAGTGGC
  TGACTTTGGCAGTGCCACATTTGACCATGAGCACCACACCACCATTGTGGCCACCCGTCACTATCGCCCG
  CCTGAGGTGATCCTTGAGCTGGGCTGGGCACAGCCCTGTGACGTCTGGAGCATTGGCTGCATTCTCTTTG
  AGTACTACCGGGGCTTCACACTCTTCCAGACCCACGAAAACCGAGAGCACCTGGTGATGATGGAGAAGAT
  CCTAGGGCCCATCCCATCACACATGATCCACCGTACCAGGAAGCAGAAATATTTCTACAAAGGGGGCCTA
  GTTTGGGATGAGAACAGCTCTGACGGCCGGTATGTGAAGGAGAACTGCAAACCTCTGAAGAGTTACATGC
  TCCAAGACTCCCTGGAGCACGTGCAGCTGTTTGACCTGATGAGGAGGATGTTAGAATTTGACCCTGCCCA
  GCGCATCACACTGGCCGAGGCCCTGCTGCACCCCTTCTTTGCTGGCCTGACCCCTGAGGAGCGGTCCTTC
  CACACCAGCCGCAACCCAAGCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226050 representing NM_001130028
  Red=Cloning site Green=Tags(s)
  
  MPVLSARRRELADHAGSGRRSGPSPTARSGPHLSALRAQPARAAHLSGRGTYVRRDTAGGGPGQARPLGP
  PGTSLLGRGARRSGEGWCPGAFESGARAARPPSRVEPRLATAASREGAGLPRAEVAAGSGRGARSGEWGL
  AAAGAWETMHHCKRYRSPEPDPYLSYRWKRRRSYSREHEGRLRYPSRREPPPRRSRSRSHDRLPYQRRYR
  ERRDSDTYRCEERSPSFGEDYYGPSRSRHRRRSRERGPYRTRKHAHHCHKRRTRSCSSASSRSQQSSKRS
  SRSVEDDKEGHLVCRIGDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEI
  NVLKKIKEKDKENKFLCVLMSDWFNFHGHMCIAFELLGKNTFEFLKENNFQPYPLPHVRHMAYQLCHALR
  FLHENQLTHTDLKPENILFVNSEFETLYNEHKSCEEKSVKNTSIRVADFGSATFDHEHHTTIVATRHYRP
  PEVILELGWAQPCDVWSIGCILFEYYRGFTLFQTHENREHLVMMEKILGPIPSHMIHRTRKQKYFYKGGL
  VWDENSSDGRYVKENCKPLKSYMLQDSLEHVQLFDLMRRMLEFDPAQRITLAEALLHPFFAGLTPEERSF
  HTSRNPSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130028
• ORF Size: 1914 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130028.1, NP_001123500.1
• RefSeq ORF: 1473 bp
• Locus ID: 1198
• UniProt ID: P49761
• Cytogenetics: 15q24.1
• Protein Families: Druggable Genome, Protein Kinase
• MW: 73.3 kDa
• Gene Summary: This gene encodes a protein belonging to the serine/threonine type protein kinase family. This protein is a nuclear dual-specificity kinase that regulates the intranuclear distribution of the serine/arginine-rich (SR) family of splicing factors. Two transcript variants encoding different isoforms have been found for this gene. Related pseudogenes are located on chromosomes 1 and 9. [provided by RefSeq, Jul 2008]