SIGLEC11 (NM_001135163) Human Tagged ORF Clone

CAT#: RC226010

SIGLEC11 (Myc-DDK-tagged)-Human sialic acid binding Ig-like lectin 11 (SIGLEC11), transcript variant 2

Product Images

Circular map for RC226010

• 

  Western blot validation of overexpression lysate (Cat# LY427568) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226010 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIGLEC11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226010 representing NM_001135163
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCCGGGACAGGCCCAGCCCCAGAGCCCAGAGATGCTGCTGCTGCCCCTGCTGCTGCCCGTGCTGG
  GGGCGGGGTCCCTGAACAAGGATCCCAGTTACAGTCTTCAAGTGCAGAGGCAGGTGCCGGTGCCGGAGGG
  CCTGTGTGTCATCGTGTCTTGCAACCTCTCCTACCCCCGGGATGGCTGGGACGAGTCTACTGCTGCTTAT
  GGCTACTGGTTCAAAGGACGGACCAGCCCAAAGACGGGTGCTCCTGTGGCCACTAACAACCAGAGTCGAG
  AGGTGGAAATGAGCACCCGGGACCGATTCCAGCTCACTGGGGATCCCGGCAAAGGGAGCTGCTCCTTGGT
  GATCAGAGACGCGCAGAGGGAGGATGAGGCATGGTACTTCTTTCGGGTGGAGAGAGGAAGCCGTGTGAGA
  CATAGTTTCCTGAGCAATGCGTTCTTTCTAAAAGTAACAGCCCTGACTAAGAAGCCTGATGTCTACATCC
  CCGAGACCCTGGAGCCCGGGCAGCCGGTGACGGTCATCTGTGTGTTTAACTGGGCTTTCAAGAAATGTCC
  AGCCCCTTCTTTCTCCTGGACGGGGGCTGCCCTCTCCCCTAGAAGAACCAGACCAAGCACCTCCCACTTC
  TCAGTGCTCAGCTTCACGCCCAGCCCCCAGGACCACGACACCGACCTCACCTGCCATGTGGACTTCTCCA
  GAAAGGGTGTGAGCGCACAGAGGACCGTCCGACTCCGTGTGGCCTATGCCCCCAAAGACCTTATTATCAG
  CATTTCACATGACAACACGTCAGCCCTGGAACTCCAGGGAAACGTCATATATCTGGAAGTTCAGAAAGGC
  CAGTTCCTGCGGCTCCTCTGTGCTGCTGACAGCCAGCCCCCTGCCACGCTGAGCTGGGTCCTGCAGGACA
  GAGTCCTCTCCTCGTCCCACCCCTGGGGCCCCAGAACCCTGGGGCTGGAGCTGCGTGGGGTAAGGGCCGG
  GGATTCAGGGCGCTACACCTGCCGAGCGGAGAACAGGCTTGGCTCCCAGCAGCAAGCCCTGGACCTCTCT
  GTGCAGTATCCTCCAGAGAACCTGAGAGTGATGGTTTCCCAAGCAAACAGGACAGTCCTGGAAAACCTCG
  GGAACGGCACATCCCTCCCGGTCCTGGAGGGCCAAAGCCTGCGCCTGGTCTGTGTCACCCACAGCAGCCC
  CCCAGCCAGGCTGAGCTGGACCCGGTGGGGACAGACCGTGGGCCCCTCCCAGCCCTCAGACCCCGGGGTC
  CTGGAGCTGCCACCCATTCAAATGGAGCACGAAGGAGAGTTCACCTGCCACGCTCAGCACCCTCTGGGCT
  CCCAGCACGTCTCTCTCAGCCTCTCCGTGCACTGGAAGCTGGAGCATGGGGGAGGACTTGGCCTGGGGGC
  TGCCCTGGGAGCTGGCGTCGCTGCCCTGCTCGCTTTCTGTTCCTGCCTTGTCGTCTTCAGGGTGAAGATC
  TGCAGGAAGGAAGCTCGCAAGAGGGCAGCAGCTGAGCAGGACGTGCCCTCCACCCTGGGACCCATCTCCC
  AGGGTCACCAGCATGAATGCTCGGCAGGCAGCTCCCAAGACCACCCGCCCCCAGGTGCAGCCACCTACAC
  CCCGGGGAAGGGGGAAGAGCAGGAGCTCCACTATGCCTCCCTCAGCTTCCAGGGCCTGAGGCTCTGGGAG
  CCTGCGGACCAGGAGGCCCCCAGCACCACCGAGTACTCGGAGATCAAGATCCACACAGGACAGCCCCTGA
  GGGGCCCAGGCTTTGGGCTTCAATTGGAGAGGGAGATGTCAGGGATGGTTCCAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226010 representing NM_001135163
  Red=Cloning site Green=Tags(s)
  
  MVPGQAQPQSPEMLLLPLLLPVLGAGSLNKDPSYSLQVQRQVPVPEGLCVIVSCNLSYPRDGWDESTAAY
  GYWFKGRTSPKTGAPVATNNQSREVEMSTRDRFQLTGDPGKGSCSLVIRDAQREDEAWYFFRVERGSRVR
  HSFLSNAFFLKVTALTKKPDVYIPETLEPGQPVTVICVFNWAFKKCPAPSFSWTGAALSPRRTRPSTSHF
  SVLSFTPSPQDHDTDLTCHVDFSRKGVSAQRTVRLRVAYAPKDLIISISHDNTSALELQGNVIYLEVQKG
  QFLRLLCAADSQPPATLSWVLQDRVLSSSHPWGPRTLGLELRGVRAGDSGRYTCRAENRLGSQQQALDLS
  VQYPPENLRVMVSQANRTVLENLGNGTSLPVLEGQSLRLVCVTHSSPPARLSWTRWGQTVGPSQPSDPGV
  LELPPIQMEHEGEFTCHAQHPLGSQHVSLSLSVHWKLEHGGGLGLGAALGAGVAALLAFCSCLVVFRVKI
  CRKEARKRAAAEQDVPSTLGPISQGHQHECSAGSSQDHPPPGAATYTPGKGEEQELHYASLSFQGLRLWE
  PADQEAPSTTEYSEIKIHTGQPLRGPGFGLQLEREMSGMVPK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135163
• ORF Size: 1806 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135163.1, NP_001128635.1
• RefSeq ORF: 1809 bp
• Locus ID: 114132
• UniProt ID: Q96RL6
• Cytogenetics: 19q13.33
• Protein Families: Druggable Genome, Transmembrane
• MW: 65.6 kDa
• Gene Summary: This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. This family member mediates anti-inflammatory and immunosuppressive signaling. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]