MSF (SEPT9) (NM_001113491) Human Tagged ORF Clone

CAT#: RC225988

SEPT9 (Myc-DDK-tagged)-Human septin 9 (SEPT9), transcript variant 1

Product Images

Circular map for RC225988

• 

  Western blot validation of overexpression lysate (Cat# LY426417) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225988 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MSF
• Synonyms: AF17q25; MSF; MSF1; NAPB; PNUTL4; SEPT9; SeptD1; SINT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225988 representing NM_001113491
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAGTCTTACTCAGGAGGCACGCGGACCTCCAGTGGCCGGCTCCGGAGGCTTGGTGACTCCAGTG
  GCCCAGCCTTGAAAAGATCTTTTGAGGTCGAGGAGGTCGAGACACCCAACTCCACCCCACCCCGGAGGGT
  CCAGACTCCCCTACTCCGAGCCACTGTGGCCAGCTCCACCCAGAAATTCCAGGACCTGGGCGTGAAGAAC
  TCAGAACCCTCGGCCCGCCATGTGGACTCCCTAAGCCAACGCTCCCCCAAGGCGTCCCTGCGGAGGGTGG
  AGCTCTCGGGCCCCAAGGCGGCCGAGCCGGTGTCCCGGCGCACTGAGCTGTCCATTGACATCTCGTCCAA
  GCAGGTGGAGAACGCCGGGGCCATCGGCCCGTCCCGGTTCGGGCTCAAGAGGGCCGAGGTGTTGGGCCAC
  AAGACGCCAGAACCGGCCCCTCGGAGGACGGAGATCACCATCGTCAAACCCCAGGAGTCAGCCCACCGGA
  GGATGGAGCCCCCTGCCTCCAAGGTCCCCGAGGTGCCCACTGCCCCTGCCACCGACGCAGCCCCCAAGAG
  GGTGGAGATCCAGATGCCCAAGCCTGCTGAGGCGCCCACCGCCCCCAGCCCAGCCCAGACCTTGGAGAAT
  TCAGAGCCTGCCCCTGTGTCTCAGCTGCAGAGCAGGCTGGAGCCCAAGCCCCAGCCCCCTGTGGCTGAGG
  CTACACCCCGGAGCCAGGAGGCCACTGAGGCGGCTCCCAGCTGCGTTGGCGACATGGCCGACACCCCCAG
  AGATGCCGGGCTCAAGCAGGCGCCTGCATCACGGAACGAGAAGGCCCCGGTGGACTTCGGCTACGTGGGG
  ATTGACTCCATCCTGGAGCAGATGCGCCGGAAGGCCATGAAGCAGGGCTTCGAGTTCAACATCATGGTGG
  TCGGGCAGAGCGGCTTGGGTAAATCCACCTTAATCAACACCCTCTTCAAATCCAAAATCAGCCGGAAGTC
  GGTGCAGCCCACCTCAGAGGAGCGCATCCCCAAGACCATCGAGATCAAGTCCATCACGCACGATATTGAG
  GAGAAAGGCGTCCGGATGAAGCTGACAGTGATTGACACACCAGGGTTCGGGGACCACATCAACAACGAGA
  ACTGCTGGCAGCCCATCATGAAGTTCATCAATGACCAGTACGAGAAATACCTGCAGGAGGAGGTCAACAT
  CAACCGCAAGAAGCGCATCCCGGACACCCGCGTCCACTGCTGCCTCTACTTCATCCCCGCCACCGGCCAC
  TCCCTCAGGCCCCTGGACATCGAGTTTATGAAACGCCTGAGCAAGGTGGTCAACATCGTCCCTGTCATCG
  CCAAGGCGGACACACTCACCCTGGAGGAGAGGGTCCACTTCAAACAGCGGATCACCGCAGACCTGCTGTC
  CAACGGCATCGACGTGTACCCCCAGAAGGAATTTGATGAGGACTCGGAGGACCGGCTGGTGAACGAGAAG
  TTCCGGGAGATGATCCCATTTGCTGTGGTGGGCAGTGACCACGAGTACCAGGTCAACGGCAAGAGGATCC
  TTGGGAGGAAGACCAAGTGGGGTACCATCGAAGTTGAAAACACCACACACTGTGAGTTTGCCTACCTGCG
  GGACCTTCTCATCAGGACGCACATGCAGAACATCAAGGACATCACCAGCAGCATCCACTTCGAGGCGTAC
  CGTGTGAAGCGCCTCAACGAGGGCAGCAGCGCCATGGCCAACGGCATGGAGGAGAAGGAGCCAGAAGCCC
  CGGAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225988 representing NM_001113491
  Red=Cloning site Green=Tags(s)
  
  MKKSYSGGTRTSSGRLRRLGDSSGPALKRSFEVEEVETPNSTPPRRVQTPLLRATVASSTQKFQDLGVKN
  SEPSARHVDSLSQRSPKASLRRVELSGPKAAEPVSRRTELSIDISSKQVENAGAIGPSRFGLKRAEVLGH
  KTPEPAPRRTEITIVKPQESAHRRMEPPASKVPEVPTAPATDAAPKRVEIQMPKPAEAPTAPSPAQTLEN
  SEPAPVSQLQSRLEPKPQPPVAEATPRSQEATEAAPSCVGDMADTPRDAGLKQAPASRNEKAPVDFGYVG
  IDSILEQMRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIE
  EKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGH
  SLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVHFKQRITADLLSNGIDVYPQKEFDEDSEDRLVNEK
  FREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSSIHFEAY
  RVKRLNEGSSAMANGMEEKEPEAPEM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113491
• ORF Size: 1758 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113491.2
• RefSeq ORF: 1761 bp
• Locus ID: 10801
• UniProt ID: Q9UHD8
• Cytogenetics: 17q25.3
• Protein Families: Druggable Genome
• MW: 65.2 kDa
• Gene Summary: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2009]