INSYN2B (NM_001129891) Human Tagged ORF Clone

CAT#: RC225919

FAM196B (Myc-DDK-tagged)-Human family with sequence similarity 196, member B (FAM196B)

Product Images

Circular map for RC225919

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: INSYN2B
• Synonyms: C5orf57; FAM196B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225919 representing NM_001129891
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGCAAAATATGAAAGTGAGACCTGTGCTTCTAAAGCGTAACAGTCTAGAATCAGTGGAGTTTG
  TGAAACAACCTCACCACCGCAGGAGCAAATCCCAGCAGGTGAGATTCAAGGAAGATGGGACCACTAAGAA
  TCCAACTGGCCTAGCTGAGGTTGACGTCCAAACTCCAGAAGACCCGGCTGTGATGGGGAAGACTCAAGCA
  ACCAGGCACCATCTTCCCCCCACCTACTCGCTCTCCTTCCCCAGGTCCCAGAAGGCAGGGGGCTTTCGCA
  ACATTGCAATCCAAACTTCCCCCAGTCTCAGGAAGCATTTCCCAGTTTTCAAAAGGAAGAGACTCACAGC
  CAGTAAGTCCCTGGTGGAAATGCCAACAGCCTCCCAAAGTGCCATCCAGGTCAACGGTAACCTCTCTGAA
  CAGGACATTGTGTCTTCTGACCTTGCCTACTTAAGGTTGGCTCAGCATCTTGAGGATGGGCCTCGAAGGG
  TCAAGGTGTCCCATGCATTCCTCCCAAGGGTCCCCAAGGTGCAAAGCAATGGTCCTGTTAGCATATGCTT
  GGAAGCAGGAACTTGGAGGTCCTTAGAGAAAGCCACAGCTGCCATTCAGGTTCCAGATGATATTTATCAC
  AGTCCTTCCTGGGAAGCTAGAGAGTCTGCTCTCAGCCCAGACAGGTCAGCTGAAGTAAGTAACTCCATAC
  ACCCTTTGGATGACACACGTCCAGGTGATGGGAGAAGGGTGACTCCACTAGATTCAGAAAAATCCACCTC
  CTGCTTAAATGCCACCAGCGTTGCCAGCCACACACCAGGCACAGAGGAACTTAAACCTGAATTGCTTTTG
  CCCAAAGACAACTCAGATGACAAAGACCTTGGCTCACTGTCATCTCAGTCAAAGGAAACGTGTGTTCCTT
  CATCTCCACGGACTCACAGTTCCCCCTCACAAGGCTCCCACAGCCAGCCAGCCCACCCAGGAAGAGCCTC
  AGACTGTCCTTCATCAAGTAACAATCACCAGAATCTGGTGTCACTCAAAACTAACAGTGCATCGAAATCT
  GCCCCTGGGTGTCAGGAGCAGACGGCAAACAACCCCACCGAGTCAGACACACTGGAGTTTCCAAATTGTC
  CAGGAAGTAATCATCTCCCATCCTCTCTTTCAAGGAGTGAGACCAAACTTCAGAGCAACAGGGAGATTAG
  TGACATTAATCAAATTCACCTGGCACGGGGTGAACTCTGCGACCTCCAAGGCCGACTGCAATCTGTGGAG
  GAATCTCTGCACTCGAACCAAGAGAAAATTAAAGTCCTTTTGAATGTAATTCAAGACTTGGAGAAAGCTC
  GAGCTCTCACTGAAGGGCGCAACTTTTATCGAACTGGCCAAGATCTCAACAATTGCAGTACCTGCCAGAA
  CACGGCGTGCATCATATACAGTGTAGAGTATGATTTTCGGCAGCAGGAAGGCAGGTTCCATGAAGTTTTG
  CAGAGTCTGGAGGAAGCAGAGCCAGTTGAGGAGGCATCGCCCCCACCAAAGTCCCCAGCAGAACCCCCAG
  CCCCGGAAAAGCAGGACTTAAGGCGGAAAACCAAGAAGGTGAAAAAGAAATGCTTCTGGTGGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225919 representing NM_001129891
  Red=Cloning site Green=Tags(s)
  
  MAQQNMKVRPVLLKRNSLESVEFVKQPHHRRSKSQQVRFKEDGTTKNPTGLAEVDVQTPEDPAVMGKTQA
  TRHHLPPTYSLSFPRSQKAGGFRNIAIQTSPSLRKHFPVFKRKRLTASKSLVEMPTASQSAIQVNGNLSE
  QDIVSSDLAYLRLAQHLEDGPRRVKVSHAFLPRVPKVQSNGPVSICLEAGTWRSLEKATAAIQVPDDIYH
  SPSWEARESALSPDRSAEVSNSIHPLDDTRPGDGRRVTPLDSEKSTSCLNATSVASHTPGTEELKPELLL
  PKDNSDDKDLGSLSSQSKETCVPSSPRTHSSPSQGSHSQPAHPGRASDCPSSSNNHQNLVSLKTNSASKS
  APGCQEQTANNPTESDTLEFPNCPGSNHLPSSLSRSETKLQSNREISDINQIHLARGELCDLQGRLQSVE
  ESLHSNQEKIKVLLNVIQDLEKARALTEGRNFYRTGQDLNNCSTCQNTACIIYSVEYDFRQQEGRFHEVL
  QSLEEAEPVEEASPPPKSPAEPPAPEKQDLRRKTKKVKKKCFWWI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001129891
• ORF Size: 1605 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001129891.3
• RefSeq ORF: 1608 bp
• Locus ID: 100131897
• UniProt ID: A6NMK8
• Cytogenetics: 5q35.1
• MW: 59 kDa