WDR26 (NM_001115113) Human Tagged ORF Clone

CAT#: RC225856

WDR26 (Myc-DDK-tagged)-Human WD repeat domain 26 (WDR26), transcript variant 2

Product Images

Circular map for RC225856

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  Western blot validation of overexpression lysate (Cat# LY426522) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225856 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified WDR26 protein (Cat# TP325856). The protein was produced from HEK293T cells transfected with WDR26 cDNA clone (Cat# RC225856) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR26
• Synonyms: CDW2; GID7; MIP2; SKDEAS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225856 representing NM_001115113
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGAGTCAGGATGTCGTTTAGAACATCCTTCTGCTACCAAATTCCGAAATCATGTCATGGAAGGAG
  ACTGGGATAAGGCAGAAAATGACCTGAATGAACTAAAGCCTTTAGTGCATTCTCCTCATGCTATTGTGAG
  GATGAAGTTTTTGCTGCTGCAGCAGAAGTACCTAGAATACCTGGAGGATGGCAAGGTCCTGGAGGCACTT
  CAAGTTCTACGCTGTGAATTGACGCCGCTGAAATACAATACAGAGCGCATTCATGTTCTTAGTGGGTATC
  TGATGTGTAGCCATGCAGAAGACCTACGTGCAAAAGCAGAATGGGAAGGCAAAGGGACAGCTTCCCGATC
  TAAACTATTGGATAAACTTCAGACCTATTTACCACCATCAGTGATGCTTCCCCCACGGCGTTTACAGACT
  CTCCTGCGGCAGGCGGTGGAACTACAAAGGGATCGGTGCCTATATCACAATACCAAACTTGATAATAATC
  TAGATTCTGTGTCTCTGCTTATAGACCATGTTTGTAGTAGGAGGCAGTTCCCATGTTATACGCAGCAGAT
  ACTTACGGAGCATTGTAATGAAGTGTGGTTCTGTAAATTCTCTAATGATGGCACTAAACTAGCAACAGGA
  TCAAAAGATACAACAGTTATCATATGGCAAGTTGATCCGGATACACACCTGCTAAAACTGCTTAAAACAT
  TAGAAGGACATGCTTATGGCGTTTCTTATATTGCATGGAGTCCAGATGACAACTATCTTGTTGCTTGTGG
  CCCAGATGACTGCTCTGAGCTTTGGCTTTGGAATGTACAAACAGGAGAACTAAGGACAAAAATGAGCCAG
  TCTCATGAAGACAGTTTGACAAGTGTGGCTTGGAATCCAGATGGGAAGCGCTTTGTGACTGGAGGTCAGC
  GTGGGCAGTTCTATCAGTGTGACTTAGATGGTAATCTCCTTGACTCCTGGGAAGGGGTAAGAGTGCAATG
  CCTTTGGTGCTTGAGTGATGGAAAGACTGTTCTGGCATCAGATACACACCAGCGAATTCGGGGCTATAAC
  TTCGAGGACCTTACAGATAGGAACATAGTACAAGAAGATCATCCTATTATGTCTTTTACTATTTCAAAAA
  ATGGCCGATTAGCTTTGTTAAATGTAGCAACTCAGGGAGTTCATTTATGGGACTTGCAAGACAGAGTTTT
  AGTAAGAAAGTATCAAGGTGTTACACAAGGGTTTTATACAATTCATTCATGTTTTGGAGGCCATAATGAA
  GACTTCATCGCTAGTGGCAGTGAAGATCACAAGGTTTACATCTGGCACAAACGTAGTGAACTGCCAATTG
  CGGAGCTGACAGGGCACACACGTACAGTAAACTGTGTGAGCTGGAACCCACAGATTCCATCCATGATGGC
  CAGCGCCTCAGATGATGGCACTGTTAGAATATGGGGACCAGCACCTTTTATAGACCACCAGAATATTGAA
  GAGGAATGCAGTAGCATGGATAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225856 representing NM_001115113
  Red=Cloning site Green=Tags(s)
  
  MQESGCRLEHPSATKFRNHVMEGDWDKAENDLNELKPLVHSPHAIVRMKFLLLQQKYLEYLEDGKVLEAL
  QVLRCELTPLKYNTERIHVLSGYLMCSHAEDLRAKAEWEGKGTASRSKLLDKLQTYLPPSVMLPPRRLQT
  LLRQAVELQRDRCLYHNTKLDNNLDSVSLLIDHVCSRRQFPCYTQQILTEHCNEVWFCKFSNDGTKLATG
  SKDTTVIIWQVDPDTHLLKLLKTLEGHAYGVSYIAWSPDDNYLVACGPDDCSELWLWNVQTGELRTKMSQ
  SHEDSLTSVAWNPDGKRFVTGGQRGQFYQCDLDGNLLDSWEGVRVQCLWCLSDGKTVLASDTHQRIRGYN
  FEDLTDRNIVQEDHPIMSFTISKNGRLALLNVATQGVHLWDLQDRVLVRKYQGVTQGFYTIHSCFGGHNE
  DFIASGSEDHKVYIWHKRSELPIAELTGHTRTVNCVSWNPQIPSMMASASDDGTVRIWGPAPFIDHQNIE
  EECSSMDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001115113
• ORF Size: 1494 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001115113.1, NP_001108585.1
• RefSeq ORF: 1938 bp
• Locus ID: 80232
• UniProt ID: Q9H7D7
• Cytogenetics: 1q42.11-q42.12
• MW: 56.94 kDa
• Gene Summary: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]