ABLIM2 (NM_001130088) Human Tagged ORF Clone

CAT#: RC225796

ABLIM2 (Myc-DDK-tagged)-Human actin binding LIM protein family, member 2 (ABLIM2), transcript variant 7

Product Images

Circular map for RC225796

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  Western blot validation of overexpression lysate (Cat# LY427156) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225796 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ABLIM2 protein (Cat# TP325796). The protein was produced from HEK293T cells transfected with ABLIM2 cDNA clone (Cat# RC225796) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABLIM2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225796 representing NM_001130088
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGCAGTGTCGCAGCCCCAGGCTGCTCCCAGCCCGCTGGAGAAGTCGCCCAGCACGGCGATCCTGT
  GCAACACGTGTGGGAATGTGTGCAAGGGCGAGGTGCTGCGGGTGCAGGACAAGTACTTCCACATCAAGTG
  CTTCGTCTGTAAAGCATGTGGCTGCGACCTGGCCGAGGGCGGCTTCTTCGTGCGGCAGGGCGAGTACATC
  TGCACGCTGGACTACCAGAGGCTCTACGGCACCCGCTGCTTCAGCTGCGACCAGTTCATTGAGGGTGAGG
  TGGTGTCGGCGCTGGGCAAGACCTACCACCCCGACTGCTTCGTGTGTGCCGTCTGCCGGCTGCCCTTCCC
  CCCCGGGGACCGAGTGACCTTCAACGGGAAGGAATGCATGTGCCAGAAGTGTTCCCTGCCCGTATCGGTG
  GGCAGCAGCGCGCACCTGTCCCAGGGCCTCCGAAGTTGTGGGGGCTGCGGCACAGAAATCAAGAATGGCC
  AGGCCCTGGTAGCCTTGGACAAGCACTGGCACTTGGGCTGTTTTAAGTGCAAGAGCTGTGGGAAGCTCCT
  GAATGCCGAGTACATCAGCAAGGATGGGCTGCCCTACTGCGAAGCTGACTATCACGCCAAGTTCGGCATC
  CGCTGTGACAGCTGTGAGAAATACATCACGGGGCGCGTGCTGGAGGCCGGAGAGAAGCACTACCACCCTT
  CCTGCGCGCTATGTGTCAGGTGCGGCCAGATGTTTGCAGAAGGCGAAGAGATGTATCTTCAAGGTTCCTC
  CATCTGGCATCCGGCGTGTCGACAAGCAGCCAGAACTGAAGACAGAAACAAGGAAACCAGAACTTCCTCA
  GAGAGCATCATTTCTGTCCCTGCTTCCAGCACCTCAGGGTCTCCGAGCCGTGTGATTTATGCCAAGCTTG
  GTGGTGAGATCCTGGACTACAGGGACTTGGCAGCCCTTCCTAAAAGTAAGGCCATCTATGACATCGACCG
  CCCCGACATGATCTCCTACTCACCCTACATCAGCCACTCTGCAGGGGACAGGCAGAGCTACGGCGAGTCC
  CCTCAGTTGCTCTCGCCAACGCCGACCGAGGGGGATCAGGATGACCGGTCCTACAAGCAGTGTCGGACCT
  CCAGCCCAAGCTCCACTGGGTCGGTTAGCCTCGGGCGCTACACTCCGACCTCACGGTCACCACAGCACTA
  CAGCCGTCCAGCTGCCAGGCGATCGGATGGGGAGGATGGAAGCTTGGACCAGGATAACAGGAAGCAGAAG
  AGCAGCTGGCTGATGCTCAAGGGGGATGCAGACACAAGGACCAATTCTCCAGACCTGGACACCCAGTCCT
  TGTCCCACAGCAGCGGGACCGACAGAGACCCTCTCCAAAGGATGGCAGGGGACAGCTTTCACTCACGTGA
  GTGGTTCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225796 representing NM_001130088
  Red=Cloning site Green=Tags(s)
  
  MSAVSQPQAAPSPLEKSPSTAILCNTCGNVCKGEVLRVQDKYFHIKCFVCKACGCDLAEGGFFVRQGEYI
  CTLDYQRLYGTRCFSCDQFIEGEVVSALGKTYHPDCFVCAVCRLPFPPGDRVTFNGKECMCQKCSLPVSV
  GSSAHLSQGLRSCGGCGTEIKNGQALVALDKHWHLGCFKCKSCGKLLNAEYISKDGLPYCEADYHAKFGI
  RCDSCEKYITGRVLEAGEKHYHPSCALCVRCGQMFAEGEEMYLQGSSIWHPACRQAARTEDRNKETRTSS
  ESIISVPASSTSGSPSRVIYAKLGGEILDYRDLAALPKSKAIYDIDRPDMISYSPYISHSAGDRQSYGES
  PQLLSPTPTEGDQDDRSYKQCRTSSPSSTGSVSLGRYTPTSRSPQHYSRPAARRSDGEDGSLDQDNRKQK
  SSWLMLKGDADTRTNSPDLDTQSLSHSSGTDRDPLQRMAGDSFHSREWFF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130088
• ORF Size: 1410 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130088.2
• RefSeq ORF: 1413 bp
• Locus ID: 84448
• UniProt ID: Q6H8Q1
• Cytogenetics: 4p16.1
• Protein Pathways: Axon guidance
• MW: 51.5 kDa
• Gene Summary: May act as scaffold protein. May stimulate ABRA activity and ABRA-dependent SRF transcriptional activity.[UniProtKB/Swiss-Prot Function]