CPA5 (NM_001127441) Human Tagged ORF Clone

CAT#: RC225716

CPA5 (Myc-DDK-tagged)-Human carboxypeptidase A5 (CPA5), transcript variant 2

Product Images

Circular map for RC225716

• 

  Western blot validation of overexpression lysate (Cat# LY426784) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225716 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPA5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225716 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGCACTCCTGGAGGCGGGACGCGCCCTGGGCCATCCCCCGTGGACAGGCGGACACTCCTGGTCT
  TCAGCTTTATCCTGGCAGCAGCTTTGGGCCAAATGAATTTCACAGGGGACCAGGTTCTTCGAGTCCTGGC
  CAAAGATGAGAAGCAGCTTTCACTTCTCGGGGATCTGGAGGGCCTGAAACCCCAGAAGGTGGACTTCTGG
  CGTGGCCCAGCCAGGCCCAGCCTCCCTGTGGATATGAGAGTTCCTTTCTCCGAACTGAAAGACATCAAAG
  CTTATCTGGAGTCTCATGGACTTGCTTACAGCATCATGATAAAGGACATCCAGGTGCTGCTGGATGAGGG
  AAGACAGGCCATGGCGAAATCCCGCCGGCTGGAGCGCAGCACCAACAGCTTCAGTTACTCATCATACCAC
  ACCCTGGAGGAGATATATAGCTGGATTGACAACTTTGTAATGGAGCATTCCGATATTGTCTCAAAAATTC
  AGATTGGCAACAGCTTTGAAAACCAGTCCATTCTTGTCCTGAAGTTCAGCACTGGAGGTTCTCGGCACCC
  AGCCATCTGGATCGACACTGGAATTCACTCCCGGGAGTGGATCACCCATGCCACCGGCATCTGGACTGCC
  AATAAGATTGTCAGTGATTATGGCAAAGACCGTGTCCTGACAGACATACTGAATGCCATGGACATCTTCA
  TAGAGCTCGTCACAAACCCTGATGGGTTTGCTTTTACCCACAGCATGAACCGCTTATGGCGGAAGAACAA
  GTCCATCAGACCTGGAATCTTCTGCATCGGCGTGGATCTCAACAGGAACTGGAAGTCGGGTTTTGGAGGA
  AATGGTTCTAACAGCAACCCCTGCTCAGAAACTTATCACGGGCCCTCCCCTCAGTCGGAGCCGGAGGTGG
  CTGCCATAGTGAACTTCATCACAGCCCATGGCAACTTCAAGGCTCTGATCTCCATCCACAGCTACTCTCA
  GATGCTTATGTACCCTTACGGCCGATTGCTGGAGCCCGTTTCAAATCAGAGGGAGTTGTACGATCTTGCC
  AAGGATGCGGTGGAGGCCTTGTATAAGGTCCATGGGATCGAGTACATTTTTGGCAGCATCAGCACCACCC
  TCTATGTGGCCAGTGGGATCACCGTCGACTGGGCCTATGACAGTGGCATCAAGTACGCCTTCAGCTTTGA
  GCTCCGGGACACTGGGCAGTATGGCTTCCTGCTGCCGGCCACACAGATCATCCCCACGGCCCAGGAGACG
  TGGATGGCGCTTCGGACCATCATGGAGCACACCCTGAATCACCCCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225716 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQGTPGGGTRPGPSPVDRRTLLVFSFILAAALGQMNFTGDQVLRVLAKDEKQLSLLGDLEGLKPQKVDFW
  RGPARPSLPVDMRVPFSELKDIKAYLESHGLAYSIMIKDIQVLLDEGRQAMAKSRRLERSTNSFSYSSYH
  TLEEIYSWIDNFVMEHSDIVSKIQIGNSFENQSILVLKFSTGGSRHPAIWIDTGIHSREWITHATGIWTA
  NKIVSDYGKDRVLTDILNAMDIFIELVTNPDGFAFTHSMNRLWRKNKSIRPGIFCIGVDLNRNWKSGFGG
  NGSNSNPCSETYHGPSPQSEPEVAAIVNFITAHGNFKALISIHSYSQMLMYPYGRLLEPVSNQRELYDLA
  KDAVEALYKVHGIEYIFGSISTTLYVASGITVDWAYDSGIKYAFSFELRDTGQYGFLLPATQIIPTAQET
  WMALRTIMEHTLNHPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127441
• ORF Size: 1308 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127441.1, NP_001120913.1
• RefSeq Size: 2078 bp
• RefSeq ORF: 1311 bp
• Locus ID: 93979
• UniProt ID: Q8WXQ8
• Cytogenetics: 7q32.2
• Protein Families: Druggable Genome, Secreted Protein
• MW: 49 kDa
• Gene Summary: Carboxypeptidases have functions ranging from digestion of food to selective biosynthesis of neuroendocrine peptides. Members of the A/B subfamily of carboxypeptidases, such as CPA5, contain an approximately 90-amino acid pro region that assists in the folding of the active carboxypeptidase domain. Cleavage of the pro region activates the enzyme (Wei et al., 2002 [PubMed 11836249]).[supplied by OMIM, Mar 2008]