RRAD (NM_001128850) Human Tagged ORF Clone

CAT#: RC225433

RRAD (Myc-DDK-tagged)-Human Ras-related associated with diabetes (RRAD), transcript variant 1

Product Images

Circular map for RC225433

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  Western blot validation of overexpression lysate (Cat# LY427008) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225433 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RRAD protein (Cat# TP325433). The protein was produced from HEK293T cells transfected with RRAD cDNA clone (Cat# RC225433) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RRAD
• Synonyms: RAD; RAD1; REM3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225433 representing NM_001128850
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCTGAACGGCGGCGGCAGCGGAGCGGGCGGGAGCCGCGGTGGGGGCCAGGAGCGCGAGCGCCGTC
  GGGGCAGCACACCCTGGGGCCCCGCCCCGCCGCTGCACCGCCGCAGCATGCCGGTGGACGAGCGCGACCT
  GCAGGCGGCGCTGACCCCGGGTGCCCTGACGGCGGCCGCGGCCGGGACGGGGACCCAGGGTCCCAGGCTG
  GACTGGCCCGAGGACTCCGAGGACTCGCTCAGCTCAGGGGGCAGCGACTCAGACGAGAGCGTTTACAAGG
  TGCTGCTGCTGGGGGCGCCCGGCGTGGGCAAGAGCGCCCTGGCGCGCATCTTCGGCGGTGTGGAGGACGG
  GCCTGAAGCAGAGGCAGCAGGGCACACCTATGATCGCTCCATTGTAGTGGACGGAGAAGAGGCATCACTC
  ATGGTCTACGACATTTGGGAGCAGGACGGGGGCCGCTGGTTGCCCGGCCACTGCATGGCCATGGGGGATG
  CCTATGTCATTGTGTACTCAGTGACGGACAAGGGCAGCTTCGAGAAGGCCTCAGAACTGCGGGTCCAGCT
  GCGGCGTGCACGGCAAACAGATGATGTGCCCATCATCCTCGTGGGCAACAAGAGCGACCTGGTGCGCTCT
  CGTGAGGTCTCGGTGGATGAGGGCCGGGCCTGCGCGGTGGTCTTTGACTGCAAGTTCATTGAGACATCAG
  CGGCATTGCACCACAATGTCCAGGCGCTGTTTGAAGGTGTCGTGCGCCAGATACGCCTGCGCAGGGACAG
  CAAAGAAGCCAACGCACGACGGCAAGCAGGCACCCGGAGGCGAGAGAGCCTTGGCAAAAAGGCGAAGCGC
  TTCTTGGGCCGCATCGTAGCTCGTAACAGCCGCAAGATGGCCTTTCGCGCCAAATCCAAGTCCTGCCACG
  ACCTCTCGGTTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225433 representing NM_001128850
  Red=Cloning site Green=Tags(s)
  
  MTLNGGGSGAGGSRGGGQERERRRGSTPWGPAPPLHRRSMPVDERDLQAALTPGALTAAAAGTGTQGPRL
  DWPEDSEDSLSSGGSDSDESVYKVLLLGAPGVGKSALARIFGGVEDGPEAEAAGHTYDRSIVVDGEEASL
  MVYDIWEQDGGRWLPGHCMAMGDAYVIVYSVTDKGSFEKASELRVQLRRARQTDDVPIILVGNKSDLVRS
  REVSVDEGRACAVVFDCKFIETSAALHHNVQALFEGVVRQIRLRRDSKEANARRQAGTRRRESLGKKAKR
  FLGRIVARNSRKMAFRAKSKSCHDLSVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001128850
• ORF Size: 924 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128850.1, NP_001122322.1
• RefSeq ORF: 927 bp
• Locus ID: 6236
• UniProt ID: P55042
• Cytogenetics: 16q22.1
• MW: 33.1 kDa
• Gene Summary: May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.[UniProtKB/Swiss-Prot Function]