CDC23 (NM_004661) Human Tagged ORF Clone

CAT#: RC224973

CDC23 (Myc-DDK-tagged)-Human cell division cycle 23 homolog (S. cerevisiae) (CDC23)

Product Images

Circular map for RC224973

• 

  Western blot validation of overexpression lysate (Cat# LY401477) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224973 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDC23
• Synonyms: ANAPC8; APC8; CUT23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224973 representing NM_004661
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGAGTACCTCCATGGTCCCGGTGGCTGTGACGGCGGCAGTGGCGCCTGTCCTGTCCATAAACA
  GCGATTTCTCAGATTTGCGGGAAATTAAAAAGCAACTGCTGCTTATTGCGGGCCTTACCCGGGAGCGGGG
  CCTACTACACAGTAGCAAATGGTCGGCGGAGTTGGCTTTCTCTCTCCCTGCATTGCCTCTGGCCGAGCTG
  CAACCGCCTCCGCCTATTACAGAGGAAGATGCCCAGGATATGGATGCCTATACCCTGGCCAAGGCCTACT
  TTGACGTTAAAGAGTATGATCGGGCAGCACATTTCCTGCATGGCTGCAATAGCAAGAAAGCCTATTTTCT
  GTATATGTATTCCAGATATCTGTCTGGAGAAAAAAAGAAGGACGATGAAACAGTTGATAGCTTAGGCCCC
  CTGGAAAAAGGACAAGTGAAAAATGAGGCGCTTAGAGAATTGAGAGTGGAGCTCAGCAAAAAACACCAAG
  CTCGAGAACTTGATGGATTTGGACTTTATCTGTATGGTGTGGTGCTTCGAAAACTGGACTTGGTTAAAGA
  GGCCATTGATGTGTTTGTGGAAGCTACTCATGTTTTGCCCTTGCATTGGGGAGCCTGGTTAGAACTCTGT
  AACCTGATCACAGACAAAGAGATGCTGAAGTTCCTGTCTTTGCCAGACACCTGGATGAAAGAGTTTTTTC
  TGGCTCATATATACACAGAGTTGCAGTTGATAGAGGAGGCCCTGCAAAAGTATCAGAATCTCATTGATGT
  GGGCTTCTCTAAGAGCTCGTATATTGTTTCCCAAATTGCAGTTGCCTATCACAATATCAGAGATATTGAC
  AAAGCCCTCTCCATTTTTAATGAGCTAAGGAAACAAGACCCTTACAGGATTGAAAATATGGACACATTCT
  CCAACCTTCTTTATGTCAGGAGCATGAAATCGGAGTTGAGTTATCTGGCTCATAACCTCTGTGAGATTGA
  TAAATATCGTGTAGAAACGTGCTGTGTAATTGGCAATTATTACAGTTTACGTTCTCAGCATGAGAAAGCA
  GCCTTATATTTCCAGAGAGCCCTGAAATTAAATCCTCGGTATCTTGGTGCCTGGACACTAATGGGACATG
  AGTACATGGAGATGAAGAACACGTCTGCTGCTATCCAGGCTTATAGACATGCCATTGAGGTCAACAAACG
  GGACTACAGAGCTTGGTATGGCCTCGGGCAGACCTATGAAATCCTTAAGATGCCATTTTACTGCCTTTAT
  TATTATAGACGGGCCCACCAGCTTCGACCCAATGATTCTCGCATGCTGGTTGCTTTAGGAGAATGTTACG
  AGAAACTCAATCAACTAGTGGAAGCCAAAAAGTGTTATTGGAGAGCTTACGCCGTGGGAGATGTGGAGAA
  AATGGCTCTGGTGAAACTGGCAAAGCTTCATGAACAGTTGACTGAGTCAGAACAGGCTGCCCAGTGTTAC
  ATCAAATATATCCAAGATATCTATTCCTGTGGGGAAATAGTAGAACACTTGGAGGAAAGCACTGCCTTTC
  GCTATCTGGCCCAGTACTATTTTAAGTGCAAACTGTGGGATGAAGCTTCAACTTGTGCACAAAAGTGTTG
  TGCATTTAATGATACCCGGGAAGAAGGTAAGGCCTTACTCCGGCAAATCCTACAGCTTCGGAACCAAGGC
  GAGACTCCTACCACCGAGGTGCCTGCTCCCTTTTTCCTACCTGCTTCACTCTCTGCTAACAATACCCCCA
  CACGCAGAGTTTCTCCACTCAACTTGTCTTCTGTCACGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224973 representing NM_004661
  Red=Cloning site Green=Tags(s)
  
  MAASTSMVPVAVTAAVAPVLSINSDFSDLREIKKQLLLIAGLTRERGLLHSSKWSAELAFSLPALPLAEL
  QPPPPITEEDAQDMDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLSGEKKKDDETVDSLGP
  LEKGQVKNEALRELRVELSKKHQARELDGFGLYLYGVVLRKLDLVKEAIDVFVEATHVLPLHWGAWLELC
  NLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQNLIDVGFSKSSYIVSQIAVAYHNIRDID
  KALSIFNELRKQDPYRIENMDTFSNLLYVRSMKSELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKA
  ALYFQRALKLNPRYLGAWTLMGHEYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLY
  YYRRAHQLRPNDSRMLVALGECYEKLNQLVEAKKCYWRAYAVGDVEKMALVKLAKLHEQLTESEQAAQCY
  IKYIQDIYSCGEIVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCAFNDTREEGKALLRQILQLRNQG
  ETPTTEVPAPFFLPASLSANNTPTRRVSPLNLSSVTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004661
• ORF Size: 1791 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004661.4
• RefSeq Size: 3169 bp
• RefSeq ORF: 1794 bp
• Locus ID: 8697
• UniProt ID: Q9UJX2
• Cytogenetics: 5q31.2
• Domains: TPR, APC8
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• MW: 68.7 kDa
• Gene Summary: The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008]