ALDH1L2 (NM_001034173) Human Tagged ORF Clone

CAT#: RC224951

ALDH1L2 (Myc-DDK-tagged)-Human aldehyde dehydrogenase 1 family, member L2 (ALDH1L2), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Circular map for RC224951

• 

  Western blot validation of overexpression lysate (Cat# LY421958) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224951 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALDH1L2
• Synonyms: mtFDH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224951 representing NM_001034173
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGGCGGGGCAGCCAGGCGCTCCGGCGCTTCTCCACTGGCCGGGTTTATTTCAAAAACAAGCTGA
  AGTTGGCACTAATTGGCCAGAGCCTCTTTGGACAAGAAGTCTATAGCCACCTCCGCAAAGAGGGCCACCG
  AGTAGTAGGGGTGTTCACAGTTCCAGACAAGGATGGAAAAGCTGACCCTCTGGCTTTGGCTGCAGAGAAA
  GATGGGACCCCTGTGTTCAAGCTTCCTAAATGGAGGGTCAAGGGCAAGACCATCAAAGAAGTGGCAGAAG
  CCTACAGATCCGTGGGTGCAGAGCTAAATGTGCTCCCTTTCTGCACTCAGTTCATTCCCATGGATATAAT
  TGATAGTCCAAAGCACGGCTCTATCATTTATCACCCATCCATCCTGCCCAGGCACAGAGGAGCCTCTGCT
  ATCAATTGGACTCTAATTATGGGAGATAAGAAAGCTGGGTTTTCTGTTTTCTGGGCTGATGATGGCTTGG
  ATACAGGACCCATCCTTCTTCAGAGATCATGTGATGTTGAACCCAATGATACAGTGGATGCACTTTATAA
  TCGGTTTCTTTTTCCTGAAGGAATCAAGGCCATGGTAGAAGCTGTCCAACTCATAGCTGATGGAAAAGCT
  CCTCGTATACCCCAGCCAGAAGAAGGGGCAACATATGAAGGTATCCAGAAAAAGGAAAATGCTGAGATTT
  CTTGGGACCAGTCTGCCGAAGTTTTACATAACTGGATTCGAGGTCATGATAAAGTCCCTGGAGCTTGGAC
  AGAGATAAATGGACAGATGGTCACTTTCTATGGCTCGACATTACTGAATAGCTCTGTGCCTCCTGGAGAA
  CCACTGGAAATTAAAGGTGCCAAGAAGCCTGGTCTCGTTACCAAAAATGGACTTGTTCTTTTTGGTAACG
  ATGGAAAAGCACTGACGGTGAGAAATCTGCAGTTTGAAGATGGAAAAATGATCCCTGCCTCTCAGTACTT
  TTCAACGGGTGAGACGTCAGTGGTAGAACTGACAGCTGAAGAGGTGAAAGTGGCAGAGACCATCAAGGTC
  ATCTGGGCTGGAATTTTAAGCAATGTCCCCATTATTGAAGACTCAACAGACTTCTTTAAATCTGGAGCAA
  GCTCAATGGATGTTGCCAGGCTGGTTGAAGAGATCAGACAGAAATGTGGTGGGCTTCAGTTGCAGAATGA
  AGATGTCTATATGGCCACCAAGTTTGAAGGCTTTATCCAAAAGGTCGTGAGGAAACTGAGAGGAGAAGAT
  CAAGAGGTGGAGCTGGTTGTAGATTATATTTCAAAGGAGGTCAATGAAATCATGGTAAAAATGCCATACC
  AGTGTTTCATAAATGGACAGTTCACAGATGCAGACGATGGAAAGACTTACGACACTATCAACCCAACAGA
  TGGATCTACAATATGCAAAGTATCCTACGCTTCTTTGGCGGATGTTGATAAAGCAGTAGCAGCAGCAAAA
  GATGCTTTTGAAAACGGTGAATGGGGAAGAATGAATGCAAGAGAAAGAGGAAGATTGATGTATAGACTTG
  CAGACCTACTGGAAGAGAACCAAGAAGAGCTGGCAACTATTGAAGCCCTTGATTCAGGGGCTGTCTATAC
  CTTGGCCCTGAAGACACACATTGGAATGTCTGTGCAAACATTCAGATATTTTGCTGGCTGGTGCGACAAA
  ATTCAGGGTTCTACTATTCCAATCAACCAGGCCCGTCCAAATCGCAATCTGACCTTCACCAAGAAAGAGC
  CACTCGGTGTCTGTGCCATTATTATTCCCTGGAACTACCCGCTGATGATGCTGGCATGGAAGAGTGCTGC
  GTGTTTGGCAGCAGGCAATACCTTAGTGCTCAAGCCAGCACAGGTCACGCCCTTGACTGCTTTGAAGTTT
  GCAGAACTGTCTGTGAAAGCAGGCTTTCCAAAGGGGGTCATCAACATCATTCCAGGCTCAGGTGGCATAG
  CAGGACAACGTCTGTCTGAACATCCTGACATCCGCAAACTTGGTTTCACTGGATCCACTCCTATTGGCAA
  ACAGATCATGAAGAGCTGTGCTGTTAGCAACTTGAAGAAAGTTTCCCTTGAGCTTGGTGGCAAGTCTCCA
  CTTATAATATTTAATGACTGTGAACTTGACAAGGCTGTGCGAATGGGCATGGGAGCAGTATTTTTCAACA
  AAGGAGAGAACTGTATTGCTGCTGGGCGGTTGTTCGTGGAAGAATCCATCCACGACGAATTTGTGACAAG
  AGTGGTAGAAGAAATTAAAAAGATGAAAATTGGTGATCCACTTGACAGATCCACTGATCATGGGCCCCAA
  AATCATAAGGCTCATCTGGAAAAGCTGCTGCAATACTGTGAAACTGGAGTGAAAGAAGGGGCCACTTTGG
  TGTACGGGGGAAGACAAGTCCAAAGGCCAGGCTTTTTCATGGAGCCGACCGTGTTCACAGATGTGGAAGA
  CTACATGTACCTCGCCAAAGAGGAATCCTTTGGGCCTATTATGGTCATTTCTAAATTCCAAAATGGGGAC
  ATCGATGGAGTGTTGCAGCGAGCAAATAGTACAGAGTATGGTTTGGCCTCAGGGGTTTTTACAAGAGACA
  TAAACAAAGCTATGTATGTGAGTGAAAAACTGGAAGCAGGAACTGTTTTTATTAACACATACAACAAGAC
  AGATGTGGCGGCCCCATTTGGCGGAGTTAAACAATCTGGCTTTGGAAAAGACTTAGGTGAGGAAGCTCTA
  AATGAATATCTCAAAACCAAGACGGTGACACTGGAATAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224951 representing NM_001034173
  Red=Cloning site Green=Tags(s)
  
  MLRRGSQALRRFSTGRVYFKNKLKLALIGQSLFGQEVYSHLRKEGHRVVGVFTVPDKDGKADPLALAAEK
  DGTPVFKLPKWRVKGKTIKEVAEAYRSVGAELNVLPFCTQFIPMDIIDSPKHGSIIYHPSILPRHRGASA
  INWTLIMGDKKAGFSVFWADDGLDTGPILLQRSCDVEPNDTVDALYNRFLFPEGIKAMVEAVQLIADGKA
  PRIPQPEEGATYEGIQKKENAEISWDQSAEVLHNWIRGHDKVPGAWTEINGQMVTFYGSTLLNSSVPPGE
  PLEIKGAKKPGLVTKNGLVLFGNDGKALTVRNLQFEDGKMIPASQYFSTGETSVVELTAEEVKVAETIKV
  IWAGILSNVPIIEDSTDFFKSGASSMDVARLVEEIRQKCGGLQLQNEDVYMATKFEGFIQKVVRKLRGED
  QEVELVVDYISKEVNEIMVKMPYQCFINGQFTDADDGKTYDTINPTDGSTICKVSYASLADVDKAVAAAK
  DAFENGEWGRMNARERGRLMYRLADLLEENQEELATIEALDSGAVYTLALKTHIGMSVQTFRYFAGWCDK
  IQGSTIPINQARPNRNLTFTKKEPLGVCAIIIPWNYPLMMLAWKSAACLAAGNTLVLKPAQVTPLTALKF
  AELSVKAGFPKGVINIIPGSGGIAGQRLSEHPDIRKLGFTGSTPIGKQIMKSCAVSNLKKVSLELGGKSP
  LIIFNDCELDKAVRMGMGAVFFNKGENCIAAGRLFVEESIHDEFVTRVVEEIKKMKIGDPLDRSTDHGPQ
  NHKAHLEKLLQYCETGVKEGATLVYGGRQVQRPGFFMEPTVFTDVEDYMYLAKEESFGPIMVISKFQNGD
  IDGVLQRANSTEYGLASGVFTRDINKAMYVSEKLEAGTVFINTYNKTDVAAPFGGVKQSGFGKDLGEEAL
  NEYLKTKTVTLEY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034173
• ORF Size: 2769 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034173.4
• RefSeq Size: 2796 bp
• RefSeq ORF: 2772 bp
• Locus ID: 160428
• UniProt ID: Q3SY69
• Cytogenetics: 12q23.3
• Protein Families: Druggable Genome
• MW: 101.6 kDa
• Gene Summary: This gene encodes a member of both the aldehyde dehydrogenase superfamily and the formyl transferase superfamily. This member is the mitochondrial form of 10-formyltetrahydrofolate dehydrogenase (FDH), which converts 10-formyltetrahydrofolate to tetrahydrofolate and CO2 in an NADP(+)-dependent reaction, and plays an essential role in the distribution of one-carbon groups between the cytosolic and mitochondrial compartments of the cell. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2010]