FBXL6 (NM_024555) Human Tagged ORF Clone

CAT#: RC224939

FBXL6 (Myc-DDK-tagged)-Human F-box and leucine-rich repeat protein 6 (FBXL6), transcript variant 2

Product Images

Circular map for RC224939

• 

  Western blot validation of overexpression lysate (Cat# LY411229) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224939 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FBXL6
• Synonyms: FBL6; FBL6A; PP14630
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224939 representing NM_024555
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCCCAGCCTCCCGGCAGGTCCGACGCAGAGCCCGGGCAGCGCCGCGGCCCCGCTCGGCCGAGG
  ACTGGTGGTGGGACCGGCTGGCGCCGAGGGGCTCGGGGTACCACCTGCTGCAGTCCGACAGCATGCTGCT
  GGTGCTGTCCGAACCCGGCCCCGCCCGGCCCCGCGCACAGCGGCGCGCTTCCCGCCGCACTCCCCGGCAG
  CCGCCCCGGGGCCCCAGCGCCGCGGCCAAGCCCAAGGCCGGGCTCAGGTCCGAGGCGGCGGCCGCGCCCG
  CACCCGCACCGGCACCCACGCCCACGCCCGAGGAAGGGCCCGACGCGGGCTGGGGAGACCGCATTCCCTT
  GGAAATCCTGGTGCAGATTTTCGGGTTGTTGGTGGCGGCGGACGGCCCCATGCCCTTCCTGGGCAGGGCT
  GCGCGCGTGTGCCGCCGCTGGCAGGAGGCCGCTTCCCAACCCGCGCTCTGGCACACCGTGACCCTGTCGT
  CCCCGCTGGTCGGCCGGCCTGCCAAGGGCGGGGTCAAGGCGGAGAAGAAGCTCCTTGCTTCCCTGGAGTG
  GCTTATGCCCAATCGGTTTTCACAGCTCCAGAGGCTGACCCTCATCCACTGGAAGTCTCAGCTGGTAGGT
  GAGTGCTGTCCTCGGCTCACTTTCCTCAAGCTCTCCGGCTGCCACGGTGTGACTGCTGACGCTCTGGTCA
  TGCTAGCCAAAGCCTGCTGCCAGCTCCATAGCCTGGACCTACAGCACTCCATGGTGGAGTCCACAGCTGT
  GGTGAGCTTCTTGGAGGAGGCAGGGTCCCGAATGCGCAAGTTGTGGCTGACCTACAGCTCCCAGACGACA
  GCCATCCTGGGCGCACTGCTGGGCAGCTGCTGCCCCCAGCTCCAGGTCCTGGAGGTGAGCACCGGCATCA
  ACCGTAATAGCATTCCCCTTCAGCTGCCTGTCGAGGCTCTGCAGAAAGGCTGCCCTCAGCTCCAGGTGCT
  GCGGCTGTTGAACCTGATGTGGCTGCCCAAGCCTCCGGGACGAGGGGTGGCTCCCGGACCAGGCTTCCCT
  AGCCTAGAGGAGCTCTGCCTGGCGAGCTCAACCTGCAACTTTGTGAGCAACGAGGTCCTGGGCCGCCTAC
  TCCACGGCTCTCCCAACCTGCGCTTACTGGATCTTCGTGGCTGTGCGCGCATCACGCCGGCTGGCCTTCA
  GGATCTGCCATGTCGGGAGCTGGAGCAGCTTCATCTGGGCCTGTATGGCACGTCAGACCGGCTGACTCTA
  GCCAAGGAGGGCAGCCCCTTTTTGACCCAGAAGTGGTGCCATACACTGCGAGAACTGGACTTGAGTGGCC
  AGGGGTTCAGTGAGAAGGACCTGGAGCAGGCCCTGGCTGCCTTCTTAAGCACCCCTGGGGGCTCACACCC
  AGCCCTGTGCTCTCTTAACCTCAGGGGCACCCGGGTCACACCAAGCACTGTCAGCTCTGTGATCAGCAGC
  TGCCCGGGCCTGCTCTACCTCAACCTGGAGTCCTGCCGCTGCCTTCCCCGGGGTCTGAAGCGGGCCTACC
  GGGGCCTGGAGGAAGTCCAGTGGTGTCTGGAGCAGCTGCTCACCAGCCCCTCACCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224939 representing NM_024555
  Red=Cloning site Green=Tags(s)
  
  MAAPASRQVRRRARAAPRPRSAEDWWWDRLAPRGSGYHLLQSDSMLLVLSEPGPARPRAQRRASRRTPRQ
  PPRGPSAAAKPKAGLRSEAAAAPAPAPAPTPTPEEGPDAGWGDRIPLEILVQIFGLLVAADGPMPFLGRA
  ARVCRRWQEAASQPALWHTVTLSSPLVGRPAKGGVKAEKKLLASLEWLMPNRFSQLQRLTLIHWKSQLVG
  ECCPRLTFLKLSGCHGVTADALVMLAKACCQLHSLDLQHSMVESTAVVSFLEEAGSRMRKLWLTYSSQTT
  AILGALLGSCCPQLQVLEVSTGINRNSIPLQLPVEALQKGCPQLQVLRLLNLMWLPKPPGRGVAPGPGFP
  SLEELCLASSTCNFVSNEVLGRLLHGSPNLRLLDLRGCARITPAGLQDLPCRELEQLHLGLYGTSDRLTL
  AKEGSPFLTQKWCHTLRELDLSGQGFSEKDLEQALAAFLSTPGGSHPALCSLNLRGTRVTPSTVSSVISS
  CPGLLYLNLESCRCLPRGLKRAYRGLEEVQWCLEQLLTSPSPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024555
• ORF Size: 1599 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024555.4
• RefSeq Size: 1781 bp
• RefSeq ORF: 1602 bp
• Locus ID: 26233
• UniProt ID: Q8N531
• Cytogenetics: 8q24.3
• Domains: LRR, F-box, LRR_CC
• MW: 57.7 kDa
• Gene Summary: This gene encodes a member of a family of proteins that are characterized by an F-box motif. The encoded protein also contains leucine-rich repeats. F-box-containing proteins comprise one of the subunits of the SCF (SKP1-cullin-F-box) complex, which functions in phosphorylation-dependent ubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]