MAGEB1 (NM_177404) Human Tagged ORF Clone

CAT#: RC224906

MAGEB1 (Myc-DDK-tagged)-Human melanoma antigen family B, 1 (MAGEB1), transcript variant 2

Product Images

Circular map for RC224906

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAGEB1 (Cat# RC224906, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAGEB1(Cat# TA800471). Positive lysates LY406154 (100ug) and LC406154 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY406154) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224906 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MAGEB1 protein (Cat# TP324906). The protein was produced from HEK293T cells transfected with MAGEB1 cDNA clone (Cat# RC224906) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAGEB1
• Synonyms: CT3.1; DAM10; MAGE-Xp; MAGEL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224906 representing NM_177404.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCTCGGGGTCAGAAGAGTAAGCTCCGTGCTCGTGAGAAACGCCGCAAGGCGCGAGAGGAGACCCAG
  GGTCTCAAGGTTGCTCACGCCACTGCAGCAGAGAAAGAGGAGTGCCCCTCCTCCTCTCCTGTTTTAGGG
  GATACTCCCACAAGCTCCCCTGCTGCTGGCATTCCCCAGAAGCCTCAGGGAGCTCCACCCACCACCACT
  GCTGCTGCAGCTGTGTCATGTACCGAATCTGACGAAGGTGCCAAATGCCAAGGTGAGGAAAATGCAAGT
  TTCTCCCAGGCCACAACATCCACTGAGAGCTCAGTCAAAGATCCTGTAGCCTGGGAGGCAGGAATGCTG
  ATGCACTTCATTCTACGTAAGTATAAAATGAGAGAGCCCATTATGAAGGCAGATATGCTGAAGGTTGTT
  GATGAAAAGTACAAGGATCACTTCACTGAGATCCTCAATGGAGCCTCTCGCCGCTTGGAGCTCGTCTTT
  GGCCTTGATTTGAAGGAAGACAACCCTAGTGGCCACACCTACACCCTCGTCAGTAAGCTAAACCTCACC
  AATGATGGAAACCTGAGCAATGATTGGGACTTTCCCAGGAATGGGCTTCTGATGCCTCTCCTGGGTGTG
  ATCTTCTTAAAGGGCAACTCTGCCACCGAGGAAGAGATCTGGAAATTCATGAATGTGTTGGGAGCCTAT
  GATGGAGAGGAGCACTTAATCTATGGGGAACCCCGTAAGTTCATCACCCAAGATCTGGTGCAGGAAAAA
  TATCTGAAGTACGAGCAGGTGCCCAACAGTGATCCCCCACGCTATCAATTCCTATGGGGTCCGAGAGCC
  TATGCTGAAACCACCAAGATGAAAGTCCTCGAGTTTTTGGCCAAGATGAATGGTGCCACTCCCCGTGAC
  TTCCCATCCCATTATGAAGAGGCTTTGAGAGATGAGGAAGAGAGAGCCCAAGTCCGATCCAGTGTTAGA
  GCCAGGCGTCGCACTACTGCCACGACTTTTAGAGCGCGTTCTAGAGCCCCATTCAGCAGGTCCTCCCAC
  CCCATG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC224906
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPRGQKSKLRAREKRRKAREETQGLKVAHATAAEKEECPSSSPVLGDTPTSSPAAGIPQKPQGAPPTTT
  AAAAVSCTESDEGAKCQGEENASFSQATTSTESSVKDPVAWEAGMLMHFILRKYKMREPIMKADMLKVV
  DEKYKDHFTEILNGASRRLELVFGLDLKEDNPSGHTYTLVSKLNLTNDGNLSNDWDFPRNGLLMPLLGV
  IFLKGNSATEEEIWKFMNVLGAYDGEEHLIYGEPRKFITQDLVQEKYLKYEQVPNSDPPRYQFLWGPRA
  YAETTKMKVLEFLAKMNGATPRDFPSHYEEALRDEEERAQVRSSVRARRRTTATTFRARSRAPFSRSSH
  PM
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC224906 also available, TP324906
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177404
• ORF Size: 1041 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177404.3
• RefSeq Size: 1708 bp
• RefSeq ORF: 1044 bp
• Locus ID: 4112
• UniProt ID: P43366
• Cytogenetics: Xp21.2
• MW: 39 kDa
• Gene Summary: This gene is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex reversal) critical region, and expressed in testis and in a significant fraction of tumors of various histological types. This gene and other MAGEB members are clustered on chromosome Xp22-p21. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene, however, the full length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]