UGT2B17 (NM_001077) Human Tagged ORF Clone

CAT#: RC224899

UGT2B17 (Myc-DDK-tagged)-Human UDP glucuronosyltransferase 2 family, polypeptide B17 (UGT2B17)

Product Images

Circular map for RC224899

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UGT2B17
• Synonyms: BMND12; UDPGT2B17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224899 representing NM_001077
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTGAAATGGATGTCAGTCTTTCTGCTGATGCAGCTCAGTTGTTACTTTAGCTCTGGGAGTTGTG
  GAAAGGTGCTGGTGTGGCCCACAGAATACAGCCATTGGATAAATATGAAGACAATCCTGGAAGAGCTTGT
  TCAGAGGGGTCATGAGGTGATTGTGTTGACATCTTCGGCTTCTATTCTTGTCAATGCCAGTAAATCATCT
  GCTATTAAATTAGAAGTTTATCCTACATCTTTAACTAAAAATGATTTGGAAGATTTTTTTATGAAAATGT
  TCGATAGATGGACATATAGTATTTCAAAAAATACATTTTGGTCATATTTTTCACAACTACAAGAATTGTG
  TTGGGAATATTCTGACTATAATATAAAGCTCTGTGAAGATGCAGTTTTGAACAAGAAACTTATGAGAAAA
  CTACAAGAGTCAAAATTTGATGTCCTTCTGGCAGATGCCGTTAATCCCTGTGGTGAGCTGCTGGCTGAGC
  TACTTAACATACCCTTTCTGTACAGTCTCCGCTTCTCTGTTGGCTACACAGTTGAGAAGAATGGTGGAGG
  ATTTCTGTTCCCTCCTTCCTATGTACCTGTTGTTATGTCAGAATTAAGTGATCAAATGATTTTCATGGAG
  AGGATAAAAAATATGATATATATGCTTTATTTTGACTTTTGGTTTCAAGCATATGATCTGAAGAAGTGGG
  ACCAGTTTTATAGTGAAGTTCTAGGAAGACCCACTACATTATTTGAGACAATGGGGAAAGCTGAAATGTG
  GCTCATTCGAACCTATTGGGATTTTGAATTTCCTCGCCCATTCTTACCAAATGTTGATTTTGTTGGAGGA
  CTTCACTGTAAACCAGCCAAACCCTTGCCTAAGGAAATGGAAGAGTTTGTGCAGAGCTCTGGAGAAAATG
  GTATTGTGGTGTTTTCTCTGGGGTCGATGATCAGTAACATGTCAGAAGAAAGTGCCAACATGATTGCATC
  AGCCCTTGCCCAGATCCCACAAAAGGTTCTATGGAGATTTGATGGCAAGAAGCCAAATACTTTAGGTTCC
  AATACTCGACTGTATAAGTGGTTACCCCAGAATGACCTTCTTGGTCATCCCAAAACCAAAGCTTTTATAA
  CTCATGGTGGAACCAATGGCATCTATGAGGCAATCTACCATGGGATCCCTATGGTGGGCATTCCCTTGTT
  TGCGGATCAACATGATAACATTGCTCACATGAAAGCCAAGGGAGCAGCCCTCAGTGTGGACATCAGGACC
  ATGTCAAGTAGAGATTTGCTCAATGCATTGAAGTCAGTCATTAATGACCCTATCTATAAAGAGAATATCA
  TGAAATTATCAAGAATTCATCATGATCAACCGGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGAGTT
  TGTCATGCGCCATAAAGGAGCCAAGCACCTTCGGGTCGCAGCCCACAACCTCACCTGGATCCAGTACCAC
  TCTTTGGATGTGATAGCATTCCTGCTGGCCTGCGTGGCAACTATGATATTTATGATCACAAAATGTTGCC
  TGTTTTGTTTCCGAAAGCTTGCCAAAACAGGAAAGAAGAAGAAAAGGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224899 representing NM_001077
  Red=Cloning site Green=Tags(s)
  
  MSLKWMSVFLLMQLSCYFSSGSCGKVLVWPTEYSHWINMKTILEELVQRGHEVIVLTSSASILVNASKSS
  AIKLEVYPTSLTKNDLEDFFMKMFDRWTYSISKNTFWSYFSQLQELCWEYSDYNIKLCEDAVLNKKLMRK
  LQESKFDVLLADAVNPCGELLAELLNIPFLYSLRFSVGYTVEKNGGGFLFPPSYVPVVMSELSDQMIFME
  RIKNMIYMLYFDFWFQAYDLKKWDQFYSEVLGRPTTLFETMGKAEMWLIRTYWDFEFPRPFLPNVDFVGG
  LHCKPAKPLPKEMEEFVQSSGENGIVVFSLGSMISNMSEESANMIASALAQIPQKVLWRFDGKKPNTLGS
  NTRLYKWLPQNDLLGHPKTKAFITHGGTNGIYEAIYHGIPMVGIPLFADQHDNIAHMKAKGAALSVDIRT
  MSSRDLLNALKSVINDPIYKENIMKLSRIHHDQPVKPLDRAVFWIEFVMRHKGAKHLRVAAHNLTWIQYH
  SLDVIAFLLACVATMIFMITKCCLFCFRKLAKTGKKKKRD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077
• ORF Size: 1590 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077.4
• RefSeq Size: 2099 bp
• RefSeq ORF: 1593 bp
• Locus ID: 7367
• UniProt ID: O75795
• Cytogenetics: 4q13.2
• Protein Families: Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, Ascorbate and aldarate metabolism, Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Pentose and glucuronate interconversions, Porphyrin and chlorophyll metabolism, Retinol metabolism, Starch and sucrose metabolism
• MW: 61.5 kDa
• Gene Summary: This gene encodes a member of the uridine diphosphoglucuronosyltransferase protein family. The encoded enzyme catalyzes the transfer of glucuronic acid from uridine diphosphoglucuronic acid to a diverse array of substrates including steroid hormones and lipid-soluble drugs. This process, known as glucuronidation, is an intermediate step in the metabolism of steroids. Copy number variation in this gene is associated with susceptibility to osteoporosis.[provided by RefSeq, Apr 2010]