Apc6 (CDC16) (NM_001078645) Human Tagged ORF Clone

CAT#: RC224869

CDC16 (Myc-DDK-tagged)-Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 2

Product Images

Circular map for RC224869

• 

  Western blot validation of overexpression lysate (Cat# LY421501) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224869 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CDC16 protein (Cat# TP324869). The protein was produced from HEK293T cells transfected with CDC16 cDNA clone (Cat# RC224869) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Apc6
• Synonyms: ANAPC6; APC6; CDC16Hs; CUT9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224869 representing NM_001078645
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTAGAGCGGCTGCGGAAGCGCGTCCGGCAGTACCTCGACCAGCAACAGTATCAAAGTGCTCTAT
  TTTGGGCAGATAAAGTAGCTTCACTCTCTCGTGAAGAACCCCAGGACATCTATTGGTTGGCTCAATGTCT
  TTACCTGACAGCACAATATCACAGAGCCGCCCATGCACTTCGGTCACGAAAACTGGACAAATTGTATGAA
  GCATGTCGTTACCTTGCAGCTAGGTGCCATTATGCTGCAAAAGAGCACCAGCAGGCCCTTGATGTTCTTG
  ACATGGAAGAGCCCATCAATAAAAGATTATTTGAAAAATACTTGAAGGATGAAAGTGGCTTCAAAGATCC
  CTCCAGCGACTGGGAAATGTCACAGTCTTCAATAAAGAGTTCTATCTGTCTTCTACGCGGGAAAATCTAT
  GATGCTCTAGATAACCGAACCCTGGCTACCTACAGCTACAAAGAAGCTTTGAAGCTTGATGTCTACTGTT
  TTGAAGCGTTCGATCTTTTAACATCACATCACATGCTGACAGCACAAGAAGAAAAAGAACTTCTTGAATC
  ACTACCCCTTAGCAAGCTGTGTAATGAAGAACAGGAATTGCTGCGTTTTCTATTTGAGAACAAATTGAAA
  AAATATAATAAGCCTAGTGAAACGGTCATCCCTGAATCTGTAGATGGCTTGCAAGAGAATCTGGATGTGG
  TAGTGTCTTTAGCTGAGAGACATTATTATAACTGTGATTTTAAAATGTGCTACAAGCTTACTTCTGTAGT
  AATGGAGAAAGATCCTTTCCATGCAAGTTGTTTACCTGTACATATAGGGACGCTTGTAGAGCTGAATAAA
  GCCAATGAACTTTTCTATCTTTCTCATAAACTGGTGGATTTATATCCTAGTAATCCTGTGTCTTGGTTTG
  CAGTGGGATGTTACTATCTCATGGTCGGTCATAAAAATGAACATGCCAGAAGATATCTCAGCAAAGCCAC
  AACACTTGAGAAAACCTATGGACCTGCATGGATAGCCTATGGACATTCATTTGCGGTGGAGAGTGAGCAC
  GACCAAGCGATGGCTGCTTACTTCACAGCAGCACAGCTGATGAAAGGGTGTCATTTGCCTATGCTGTATA
  TTGGATTAGAATATGGTTTGACCAATAACTCAAAACTAGCTGAAAGGTTCTTCAGCCAAGCTCTGAGCAT
  TGCACCGGAAGACCCTTTTGTTATGCATGAGGTCGGCGTGGTTGCATTTCAGAATGGAGAATGGAAAACA
  GCCGAAAAATGGTTTCTTGATGCTTTGGAAAAAATTAAAGCAATTGGGAACGAGGTAACAGTTGACAAAT
  GGGAACCTTTGTTGAACAACTTGGGGCATGTCTGCAGAAAACTTAAAAAGTATGCTGAGGCCTTGGATTA
  CCACCGTCAGGCACTGGTGTTGATTCCTCAGAACGCATCCACCTACTCTGCTATTGGATATATCCACAGT
  CTGATGGGCAACTTTGAAAATGCTGTGGACTACTTCCACACAGCCCTTGGTCTTAGGCGAGATGATACAT
  TTTCTGTTACAATGCTTGGTCATTGCATCGAAATGTACATTGGTGATTCTGAAGCTTATATCGGAGCAGA
  CATTAAAGACAAATTAAAATGTTATGACTTTGATGTGCATACAATGAAGACACTAAAAAACATTATTTCA
  CCTCCGTGGGATTTCAGGGAATTTGAAGTAGAAAAACAGACTGCAGAAGAAACGGGGCTTACGCCATTGG
  AAACCTCAAGGAAAACTCCAGATTCCAGACCTTCCTTGGAAGAAACCTTTGAAATTGAAATGAATGAAAG
  TGACATGATGTTAGAGACATCTATGTCAGACCACAGCACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224869 representing NM_001078645
  Red=Cloning site Green=Tags(s)
  
  MNLERLRKRVRQYLDQQQYQSALFWADKVASLSREEPQDIYWLAQCLYLTAQYHRAAHALRSRKLDKLYE
  ACRYLAARCHYAAKEHQQALDVLDMEEPINKRLFEKYLKDESGFKDPSSDWEMSQSSIKSSICLLRGKIY
  DALDNRTLATYSYKEALKLDVYCFEAFDLLTSHHMLTAQEEKELLESLPLSKLCNEEQELLRFLFENKLK
  KYNKPSETVIPESVDGLQENLDVVVSLAERHYYNCDFKMCYKLTSVVMEKDPFHASCLPVHIGTLVELNK
  ANELFYLSHKLVDLYPSNPVSWFAVGCYYLMVGHKNEHARRYLSKATTLEKTYGPAWIAYGHSFAVESEH
  DQAMAAYFTAAQLMKGCHLPMLYIGLEYGLTNNSKLAERFFSQALSIAPEDPFVMHEVGVVAFQNGEWKT
  AEKWFLDALEKIKAIGNEVTVDKWEPLLNNLGHVCRKLKKYAEALDYHRQALVLIPQNASTYSAIGYIHS
  LMGNFENAVDYFHTALGLRRDDTFSVTMLGHCIEMYIGDSEAYIGADIKDKLKCYDFDVHTMKTLKNIIS
  PPWDFREFEVEKQTAEETGLTPLETSRKTPDSRPSLEETFEIEMNESDMMLETSMSDHST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001078645
• ORF Size: 1860 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001078645.3
• RefSeq Size: 2317 bp
• RefSeq ORF: 1863 bp
• Locus ID: 8881
• UniProt ID: Q13042
• Cytogenetics: 13q34
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• MW: 71.5 kDa
• Gene Summary: The protein encoded by this gene functions as a protein ubiquitin ligase and is a component of the multiprotein APC complex. The APC complex is a cyclin degradation system that governs exit from mitosis by targeting cell cycle proteins for degredation by the 26S proteasome. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein, and other APC complex proteins, contain a tetratricopeptide repeat (TPR) domain; a protein domain that is often involved in protein-protein interactions and the assembly of multiprotein complexes. Multiple alternatively spliced transcript variants, encoding distinct proteins, have been identified. [provided by RefSeq, Jan 2016]