GIT1 (NM_001085454) Human Tagged ORF Clone

CAT#: RC224712

GIT1 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant 1

Product Images

Circular map for RC224712

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GIT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224712 representing NM_001085454
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGAAAGGGGCCGCGAGCGGAGGTGTGTGCGGACTGCAGCGCCCCGGACCCTGGCTGGGCATCCA
  TCAGCAGGGGTGTGCTGGTGTGTGACGAGTGCTGCAGCGTGCACCGGAGCCTGGGACGCCACATCTCCAT
  TGTCAAGCACCTTCGCCACAGCGCCTGGCCTCCCACGCTGCTGCAGATGGTGCACACGCTTGCCAGCAAC
  GGGGCCAACTCCATCTGGGAGCACTCCCTGCTGGACCCCGCACAAGTGCAGAGCGGCCGGCGTAAAGCCA
  ACCCCCAAGACAAAGTCCACCCCATCAAGTCAGAGTTCATCAGGGCCAAGTACCAGATGCTGGCATTTGT
  GCACAAGCTTCCCTGCCGGGACGATGATGGAGTCACCGCCAAAGACCTCAGCAAGCAACTACACTCGAGC
  GTGCGGACAGGCAACCTGGAGACATGTCTGCGCCTGCTCTCCCTGGGTGCCCAGGCCAACTTCTTCCACC
  CAGAGAAGGGCACCACACCTCTGCACGTGGCTGCCAAGGCAGGACAGACACTGCAGGCCGAGCTGCTTGT
  AGTGTATGGGGCTGACCCTGGCTCCCCTGATGTTAATGGCCGCACACCCATTGACTATGCCAGGCAGGCG
  GGGCACCATGAGCTGGCGGAAAGGCTGGTTGAGTGCCAATATGAGCTCACTGACCGGCTGGCCTTCTACC
  TCTGTGGACGCAAGCCGGATCACAAGAATGGGCATTACATCATCCCACAGATGGCTGACAGATCTCGGCA
  AAAGTGCATGTCTCAGAGCCTTGACTTATCCGAATTGGCCAAAGCTGCTAAGAAGAAGCTGCAGGCGCTC
  AGCAACCGGCTTTTTGAGGAACTCGCCATGGACGTGTATGACGAGGTGGATCGAAGAGAAAATGATGCAG
  TGTGGCTGGCTACCCAAAACCACAGCACTCTGGTGACAGAGCGCAGTGCCGTGCCCTTCCTGCCTGTTAA
  CCCGGAATACTCAGCCACGCGGAATCAGGGGCGACAAAAGCTGGCCCGCTTTAATGCCCGAGAGTTTGCC
  ACCTTGATCATCGACATTCTCAGTGAGGCCAAGCGGAGACAGCAGGGCAAGAGCCTGAGCAGCCCCACAG
  ACAACCTCGAGCTGTCTCTGCGGAGCCAGAGTGACCTCGACGACCAACACGACTACGACAGCGTGGCCTC
  TGACGAGGACACAGACCAGGAGCCCCTGCGCAGCACCGGCGCCACTCGGAGCAACCGGGCCCGGAGCATG
  GACTCCTCGGACTTGTCTGACGGGGCTGTGACGCTGCAGGAGTACCTGGAGCTGAAGAAGGCCCTGGCTA
  CATCGGAGGCAAAGGTGCAGCAGCTCATGAAGGTCAACAGTAGCCTGAGCGACGAGCTCCGGAGGCTGCA
  GCGAGAGATCCACAAGCTGCAGGCGGAGAACCTGCAGCTCCGGCAGCCTCCAGGGCCGGTGCCCACACCT
  CCACTCCCCAGTGAACGGGCGGAACACACACCCATGGCGCCAGGCGGGAGCACACACCGCAGGGATCGCC
  AGGCCTTTTCCATGTATGAACCTGGCTCTGCCCTGAAGCCCTTTGGGGGCCCCCCTGGGGACGAGCTCAC
  TACGCGGCTGCAGCCTTTCCACAGCACTGAGCTAGAGGACGACGCCATCTATTCAGTGCACGTCCCTGCT
  GGCCTTTACCGGATCCGGAAAGGGGTGTCTGCCTCAGCTGTGCCCTTCACTCCCTCCTCCCCGCTGCTGT
  CCTGCTCCCAGGAGGGAAGCCGCCACACGAGCAAGCTTTCCCGCCACGGCAGTGGAGCCGACAGTGACTA
  TGAGAACACGCAAAGTGGGGACCCACTGCTGGGGCTGGAAGGGAAGAGGTTTCTAGAGCTGGGCAAAGAG
  GAAGACTTCCACCCAGAGCTGGAAAGCCTGGATGGAGACCTAGATCCTGGGCTTCCCAGCACAGAGGATG
  TCATCTTGAAGACAGAGCAGGTCACCAAGAACATTCAGGAACTGTTGCGGGCAGCCCAGGAGTTCAAGCA
  TGACAGCTTCGTGCCCTGCTCAGAGAAGATCCATTTGGCTGTGACCGAGATGGCCTCCCTCTTCCCAAAG
  AGGCCAGCCCTGGAGCCAGTGCGGAGCTCACTGCGGCTGCTCAACGCCAGCGCCTACCGGCTGCAGAGTG
  AGTGCCGGAAGACAGTGCCCCCAGAGCCCGGCGCCCCAGTGGACTTCCAGCTGCTGACTCAGCAGGTGAT
  CCAGTGCGCCTATGACATCGCCAAGGCTGCCAAGCAGCTGGTCACCATCACCACCCGAGAGAAGAAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224712 representing NM_001085454
  Red=Cloning site Green=Tags(s)
  
  MSRKGPRAEVCADCSAPDPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQMVHTLASN
  GANSIWEHSLLDPAQVQSGRRKANPQDKVHPIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSS
  VRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQA
  GHHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRSRQKCMSQSLDLSELAKAAKKKLQAL
  SNRLFEELAMDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFA
  TLIIDILSEAKRRQQGKSLSSPTDNLELSLRSQSDLDDQHDYDSVASDEDTDQEPLRSTGATRSNRARSM
  DSSDLSDGAVTLQEYLELKKALATSEAKVQQLMKVNSSLSDELRRLQREIHKLQAENLQLRQPPGPVPTP
  PLPSERAEHTPMAPGGSTHRRDRQAFSMYEPGSALKPFGGPPGDELTTRLQPFHSTELEDDAIYSVHVPA
  GLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKE
  EDFHPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPK
  RPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001085454
• ORF Size: 2310 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001085454.2
• RefSeq Size: 3778 bp
• RefSeq ORF: 2313 bp
• Locus ID: 28964
• UniProt ID: Q9Y2X7
• Cytogenetics: 17q11.2
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Regulation of actin cytoskeleton
• MW: 85.3 kDa
• Gene Summary: GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve ENTR1 and PTPN13 (By similarity).[UniProtKB/Swiss-Prot Function]