ZNF336 (GZF1) (NM_022482) Human Tagged ORF Clone

CAT#: RC224698

GZF1 (Myc-DDK-tagged)-Human GDNF-inducible zinc finger protein 1 (GZF1)

Product Images

Circular map for RC224698

• 

  Western blot validation of overexpression lysate (Cat# LY411649) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224698 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF336
• Synonyms: JLSM; ZBTB23; ZNF336
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224698 representing NM_022482
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGCGGTGCAGTTCTGCTGGAATCCAAATCCTCCCCATTTAACCTACTGCATGAGATGCATGAGC
  TTCGCCTCCTGGGTCACCTGTGTGACGTGACAGTCAGCGTGGAGTATCAGGGTGTCCGCAAAGACTTCAT
  GGCCCACAAGGCAGTGCTGGCTGCCACCAGCAAGTTTTTTAAGGAAGTGTTCCTTAATGAGAAGAGTGTG
  GATGGTACTAGGACTAATGTCTACTTAAATGAAGTGCAGGTTGCTGACTTTGCTTCATTTCTTGAGTTTG
  TCTACACTGCAAAGGTACAGGTGGAAGAAGATCGGGTGCAGCGAATGCTGGAAGTGGCTGAAAAGCTGAA
  ATGTTTGGATTTATCAGAAACTTGTTTTCAATTAAAGAAACAGATGTTAGAGTCAGTACTTTTGGAGTTG
  CAAAATTTCTCAGAGTCTCAGGAGGTGGAGGTGAGCAGTGGCTCCCAAGTTAGTGCTGCTCCTGCCCCCA
  GGGCAAGTGTGGCCACCGATGGCCCTCACCCCAGTGGTCTCACGGATTCCTTGGACTACCCAGGAGAGAG
  AGCCAGCAATGGCATGTCTTCAGATTTGCCACCGAAGAAGTCCAAGGACAAACTAGACAAGAAGAAAGAG
  GTAGTTAAACCTCCCTACCCTAAAATCAGGAGAGCTAGTGGAAGGCTGGCTGGGAGGAAGGTCTTTGTGG
  AGATCCCTAAAAAGAAATATACGAGAAGACTCCGAGAGCAGCAGAAAACTGCTGAGGGTGATGTGGGGGA
  CTACAGGTGTCCCCAGGACCAAAGCCCGGACAGGGTGGGCACGGAGATGGAGCCGGTTTCCAAAAATGAG
  GGTTGCCAGGCAGGTGCTGAGTTGGAGGAATTGTCAAAGAAAGCAGGGCCGGAGGAGGAAGAGGAGGAGG
  AGGAGGAGGACGAAGAAGGGGAGAAGAAGAAGAGCAACTTTAAGTGCAGCATTTGCGAGAAGGCGTTTCT
  GTATGAGAAGAGCTTCCTGAAGCACAGCAAGCACCGCCACGGCGTGGCCACCGAGGTGGTGTACCGCTGC
  GACACCTGCGGCCAGACCTTCGCCAACCGCTGCAACCTGAAGAGCCACCAGCGCCACGTGCACAGCAGCG
  AGCGCCATTTCCCATGCGAGCTGTGCGGGAAGAAGTTCAAGCGCAAGAAGGACGTGAAGCGGCACGTGCT
  GCAGGTGCATGAGGGCGGCGGCGAGCGGCACCGCTGCGGCCAGTGCGGCAAGGGCCTGAGTTCCAAGACA
  GCGCTGCGGCTGCACGAGCGCACACACACGGGAGACCGGCCCTACGGCTGCACCGAGTGCGGCGCCAGGT
  TCTCGCAGCCGTCCGCGCTCAAGACGCACATGAGAATTCATACAGGGGAAAAACCTTTTGTCTGTGATGA
  ATGTGGTGCAAGATTCACTCAGAACCACATGCTGATTTATCATAAAAGGTGTCACACAGGTGAAAGACCT
  TTTATGTGTGAAACATGTGGCAAGAGTTTTGCTTCTAAGGAGTACTTAAAACACCACAATAGAATCCATA
  CTGGATCCAAACCCTTTAAATGTGAAGTATGTTTCAGGACTTTTGCCCAGCGGAATTCACTGTACCAGCA
  TATTAAAGTCCACACAGGGGAGCGTCCCTACTGCTGTGACCAGTGCGGCAAGCAGTTCACCCAGCTCAAC
  GCCCTCCAGCGCCACCGCCGCATCCACACAGGGGAGAGGCCATTCATGTGCAATGCGTGCGGACGGACAT
  TCACCGACAAGTCCACTCTTCGGCGGCACACCTCAATACACGATAAGAATACTCCATGGAAGTCTTTCCT
  TGTCATTGTAGATGGCTCGCCCAAGAACGATGACGGACACAAGACTGAACAGCCTGACGAAGAGTATGTG
  TCATCCAAGCTTTCGGATAAATTGCTGTCTTTTGCAGAAAATGGCCATTTCCACAACCTGGCTGCAGTCC
  AAGACACTGTACCTACCATGCAGGAGAACAGTTCTGCTGACACAGCCTGCAAGGCAGATGACTCCGTGGT
  GTCCCAGGACACCCTCCTGGCCACCACCATCAGTGAGCTTAGCGAGCTGACCCCACAGACAGACTCGATG
  CCCACACAGCTTCACTCTTTGAGCAACATGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224698 representing NM_022482
  Red=Cloning site Green=Tags(s)
  
  MESGAVLLESKSSPFNLLHEMHELRLLGHLCDVTVSVEYQGVRKDFMAHKAVLAATSKFFKEVFLNEKSV
  DGTRTNVYLNEVQVADFASFLEFVYTAKVQVEEDRVQRMLEVAEKLKCLDLSETCFQLKKQMLESVLLEL
  QNFSESQEVEVSSGSQVSAAPAPRASVATDGPHPSGLTDSLDYPGERASNGMSSDLPPKKSKDKLDKKKE
  VVKPPYPKIRRASGRLAGRKVFVEIPKKKYTRRLREQQKTAEGDVGDYRCPQDQSPDRVGTEMEPVSKNE
  GCQAGAELEELSKKAGPEEEEEEEEEDEEGEKKKSNFKCSICEKAFLYEKSFLKHSKHRHGVATEVVYRC
  DTCGQTFANRCNLKSHQRHVHSSERHFPCELCGKKFKRKKDVKRHVLQVHEGGGERHRCGQCGKGLSSKT
  ALRLHERTHTGDRPYGCTECGARFSQPSALKTHMRIHTGEKPFVCDECGARFTQNHMLIYHKRCHTGERP
  FMCETCGKSFASKEYLKHHNRIHTGSKPFKCEVCFRTFAQRNSLYQHIKVHTGERPYCCDQCGKQFTQLN
  ALQRHRRIHTGERPFMCNACGRTFTDKSTLRRHTSIHDKNTPWKSFLVIVDGSPKNDDGHKTEQPDEEYV
  SSKLSDKLLSFAENGHFHNLAAVQDTVPTMQENSSADTACKADDSVVSQDTLLATTISELSELTPQTDSM
  PTQLHSLSNME
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022482
• ORF Size: 2133 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022482.5
• RefSeq Size: 4752 bp
• RefSeq ORF: 2136 bp
• Locus ID: 64412
• UniProt ID: Q9H116
• Cytogenetics: 20p11.21
• Domains: BTB, zf-C2H2
• Protein Families: Transcription Factors
• MW: 80.3 kDa
• Gene Summary: Transcriptional repressor that binds the GZF1 responsive element (GRE) (consensus: 5'-TGCGCN[TG][CA]TATA-3'). May be regulating VSX2/HOX10 expression.[UniProtKB/Swiss-Prot Function]