Kv2.1 (KCNB1) (NM_004975) Human Tagged ORF Clone

CAT#: RC224667

KCNB1 (Myc-DDK-tagged)-Human potassium voltage-gated channel, Shab-related subfamily, member 1 (KCNB1)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro


  "NM_004975" in other vectors (6)

Reconstitution Protocol

USD 786.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

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Rabbit Polyclonal Anti-KCNB1 Antibody
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USD 380.00

Other products for "Kv2.1"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol Kv2.1
Synonyms DEE26; DRK1; Kv2.1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC224667 representing NM_004975
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGCGGGCATGACGAAGCATGGCTCCCGCTCCACCAGCTCGCTGCCGCCCGAGCCCATGGAGATCG
TGCGCAGCAAGGCGTGCTCTCGGCGGGTCCGCCTCAACGTCGGGGGGCTGGCGCACGAGGTACTCTGGCG
TACCCTGGACCGCCTGCCCCGCACGCGGCTGGGCAAGCTCCGCGACTGCAACACGCACGACTCGCTGCTC
GAGGTGTGCGATGACTACAGCCTCGACGACAACGAGTACTTCTTTGACCGCCACCCGGGCGCCTTCACCT
CCATCCTCAACTTCTACCGCACTGGGCGACTGCACATGATGGAGGAGATGTGCGCGCTCAGCTTCAGCCA
AGAGCTCGACTACTGGGGCATCGACGAGATCTACCTGGAGTCCTGCTGCCAGGCCCGCTACCACCAGAAG
AAAGAGCAGATGAACGAGGAGCTCAAGCGTGAGGCCGAGACCCTACGGGAGCGGGAAGGCGAGGAGTTCG
ATAACACGTGCTGCGCAGAGAAGAGGAAAAAACTCTGGGACCTACTGGAGAAGCCCAATTCCTCTGTGGC
TGCCAAGATCCTTGCCATAATTTCCATCATGTTCATCGTCCTCTCCACCATTGCCCTGTCCCTCAACACG
CTGCCTGAGCTACAGAGCCTCGATGAGTTCGGCCAGTCCACAGACAACCCCCAGCTGGCCCACGTGGAGG
CCGTGTGCATCGCATGGTTCACCATGGAGTACCTGCTGAGGTTCCTCTCCTCGCCCAAGAAGTGGAAGTT
CTTCAAGGGCCCACTCAATGCCATTGACTTGTTGGCCATTCTGCCATACTATGTCACCATTTTCCTCACC
GAATCCAACAAGAGCGTGCTGCAATTCCAGAATGTCCGCCGCGTGGTCCAGATCTTCCGCATCATGCGAA
TTCTCCGCATCCTTAAGCTTGCACGCCACTCCACTGGCCTCCAGTCTCTGGGCTTCACTTTGCGGAGGAG
CTACAATGAGTTGGGCTTGCTCATCCTCTTCCTTGCCATGGGCATTATGATCTTCTCCAGCCTTGTCTTC
TTTGCTGAGAAGGATGAGGACGACACCAAGTTCAAAAGCATCCCAGCCTCTTTCTGGTGGGCCACCATCA
CCATGACTACTGTTGGGTATGGAGACATCTACCCCAAGACTCTCCTGGGGAAAATTGTTGGGGGACTCTG
CTGCATTGCAGGAGTCCTGGTGATTGCTCTTCCCATCCCCATCATCGTCAATAACTTCTCTGAGTTCTAT
AAGGAGCAGAAGAGACAGGAGAAAGCAATCAAACGGCGAGAGGCTCTGGAGAGAGCCAAGAGGAATGGCA
GCATCGTATCCATGAACATGAAGGATGCTTTTGCCCGGAGCATTGAGATGATGGACATTGTGGTTGAGAA
AAATGGGGAGAATATGGGTAAGAAAGACAAAGTACAAGATAACCACTTGTCTCCTAACAAATGGAAATGG
ACAAAGAGGACACTGTCTGAAACCAGCTCAAGTAAGTCCTTTGAAACCAAGGAACAGGGATCCCCTGAAA
AAGCCAGATCGTCTTCTAGTCCTCAGCACCTGAACGTTCAGCAGTTGGAAGACATGTACAATAAGATGGC
CAAGACCCAATCCCAACCCATCCTCAATACCAAGGAGTCAGCAGCACAGAGCAAACCAAAGGAAGAACTT
GAAATGGAGAGTATCCCCAGCCCCGTAGCCCCTCTGCCCACTCGCACAGAAGGGGTCATTGACATGCGAA
GTATGTCAAGCATTGATAGTTTCATTAGCTGTGCCACAGACTTCCCTGAGGCCACCAGATTCTCCCACAG
CCCTTTGACATCACTCCCCAGCAAGACTGGGGGCAGCACAGCCCCAGAAGTGGGCTGGCGGGGAGCTCTG
GGTGCCAGTGGTGGTAGGTTTGTGGAGGCCAACCCCAGCCCTGATGCCAGCCAGCACTCTAGTTTCTTCA
TCGAGAGCCCCAAGAGTTCCATGAAAACTAACAACCCTTTGAAGCTCCGAGCACTTAAAGTCAACTTCAT
GGAGGGTGACCCCAGTCCACTCCTCCCCGTTCTAGGGATGTACCATGACCCTCTCAGGAACCGGGGGAGT
GCTGCGGCTGCTGTCGCTGGACTGGAGTGTGCCACGCTTTTGGACAAGGCTGTGCTGAGCCCAGAGTCCT
CCATCTACACCACAGCAAGTGCTAAGACACCCCCCCGGTCTCCTGAGAAACACACAGCAATAGCGTTCAA
CTTTGAGGCGGGTGTCCACCAGTACATTGACGCAGACACAGATGATGAGGGACAGCTGCTCTACAGTGTG
GACTCCAGCCCCCCCAAAAGCCTCCCTGGGAGCACCAGTCCGAAGTTCAGCACGGGGACAAGATCGGAGA
AAAACCACTTTGAAAGCTCCCCTTTACCCACCTCCCCTAAGTTCTTAAGGCAGAACTGTATTTACTCCAC
AGAAGCATTGACTGGAAAAGGCCCCAGTGGTCAGGAAAAGTGCAAACTTGAGAACCACATCTCCCCTGAC
GTCCGTGTGTTGCCAGGGGGAGGAGCCCATGGAAGCACACGAGATCAGAGCATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC224667 representing NM_004975
Red=Cloning site Green=Tags(s)

MPAGMTKHGSRSTSSLPPEPMEIVRSKACSRRVRLNVGGLAHEVLWRTLDRLPRTRLGKLRDCNTHDSLL
EVCDDYSLDDNEYFFDRHPGAFTSILNFYRTGRLHMMEEMCALSFSQELDYWGIDEIYLESCCQARYHQK
KEQMNEELKREAETLREREGEEFDNTCCAEKRKKLWDLLEKPNSSVAAKILAIISIMFIVLSTIALSLNT
LPELQSLDEFGQSTDNPQLAHVEAVCIAWFTMEYLLRFLSSPKKWKFFKGPLNAIDLLAILPYYVTIFLT
ESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVF
FAEKDEDDTKFKSIPASFWWATITMTTVGYGDIYPKTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFY
KEQKRQEKAIKRREALERAKRNGSIVSMNMKDAFARSIEMMDIVVEKNGENMGKKDKVQDNHLSPNKWKW
TKRTLSETSSSKSFETKEQGSPEKARSSSSPQHLNVQQLEDMYNKMAKTQSQPILNTKESAAQSKPKEEL
EMESIPSPVAPLPTRTEGVIDMRSMSSIDSFISCATDFPEATRFSHSPLTSLPSKTGGSTAPEVGWRGAL
GASGGRFVEANPSPDASQHSSFFIESPKSSMKTNNPLKLRALKVNFMEGDPSPLLPVLGMYHDPLRNRGS
AAAAVAGLECATLLDKAVLSPESSIYTTASAKTPPRSPEKHTAIAFNFEAGVHQYIDADTDDEGQLLYSV
DSSPPKSLPGSTSPKFSTGTRSEKNHFESSPLPTSPKFLRQNCIYSTEALTGKGPSGQEKCKLENHISPD
VRVLPGGGAHGSTRDQSI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004975
ORF Size 2574 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004975.4
RefSeq Size 3756 bp
RefSeq ORF 2577 bp
Locus ID 3745
UniProt ID Q14721
Cytogenetics 20q13.13
Domains BTB, K_tetra, Kv2channel, ion_trans
Protein Families Druggable Genome, Ion Channels: Potassium, Transmembrane
Protein Pathways Taste transduction
MW 95.7 kDa
Gene Summary Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shab-related subfamily. This member is a delayed rectifier potassium channel and its activity is modulated by some other family members. [provided by RefSeq, Jul 2008]

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