MECR (NM_016011) Human Tagged ORF Clone

CAT#: RC224634

MECR (Myc-DDK-tagged)-Human mitochondrial trans-2-enoyl-CoA reductase (MECR), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Circular map for RC224634

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  Western blot validation of overexpression lysate (Cat# LY414252) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224634 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MECR protein (Cat# TP324634). The protein was produced from HEK293T cells transfected with MECR cDNA clone (Cat# RC224634) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MECR
• Synonyms: CGI-63; DYTOABG; ETR1; FASN2B; NRBF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224634 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGTCTGCAGTACCCTGTGGCGGGTGCGAACCCCCGCCCGGCAGTGGCGGGGGCTGCTCCCAGCTT
  CTGGCTGTCACGGACCTGCCGCCTCCTCCTACTCCGCATCCGCCGAGCCTGCCCGGGTCCGGGCGCTTGT
  CTATGGGCACCACGGGGATCCAGCCAAGGTCGTCGAACTCAAGAACCTGGAGCTAGCTGCTGTGAGAGGA
  TCAGATGTCCGTGTGAAGATGCTGGCGGCCCCTATCAATCCATCTGACATAAATATGATCCAAGGAAACT
  ACGGACTCCTTCCTGAACTGCCTGCTGTTGGAGGGAACGAAGGTGTTGCACAGGTGGTAGCGGTGGGCAG
  CAATGTGACCGGGCTGAAGCCAGGAGACTGGGTGATTCCAGCAAATGCTGGTTTAGGAACCTGGCGGACC
  GAGGCTGTGTTCAGCGAGGAAGCACTGATCCAAGTTCCGAGTGACATCCCTCTTCAGAGCGCTGCCACCC
  TGGGTGTCAATCCCTGCACAGCCTACAGGATGTTGATGGACTTCGAGCAACTGCAGCCAGGGGATTCTGT
  CATCCAGAATGCATCCAACAGCGGAGTGGGGCAAGCGGTCATCCAGATCGCCGCAGCCCTGGGCCTAAGA
  ACCATCAATGTGGTCCGAGACAGACCTGATATCCAGAAGCTGAGTGACAGACTGAAGAGTCTGGGGGCTG
  AGCATGTCATCACAGAAGAGGAGCTAAGAAGGCCCGAAATGAAAAACTTCTTTAAGGACATGCCCCAGCC
  ACGGCTTGCTCTCAACTGTGTTGGTGGGAAAAGCTCCACAGAGCTGCTGCGGCAGTTAGCGCGTGGAGGA
  ACCATGGTAACCTATGGGGGGATGGCCAAGCAGCCCGTCGTAGCCTCTGTGAGCCTGCTCATTTTTAAGG
  ATCTCAAACTTCGAGGCTTTTGGTTGTCCCAGTGGAAGAAGGATCACAGTCCAGACCAGTTCAAGGAGCT
  GATCCTCACACTGTGCGATCTCATCCGCCGAGGCCAGCTCACAGCCCCTGCCTGCTCCCAGGTCCCGCTG
  CAGGACTACCAGTCTGCCTTGGAAGCCTCCATGAAGCCCTTCATATCTTCAAAGCAGATTCTCACCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224634 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWVCSTLWRVRTPARQWRGLLPASGCHGPAASSYSASAEPARVRALVYGHHGDPAKVVELKNLELAAVRG
  SDVRVKMLAAPINPSDINMIQGNYGLLPELPAVGGNEGVAQVVAVGSNVTGLKPGDWVIPANAGLGTWRT
  EAVFSEEALIQVPSDIPLQSAATLGVNPCTAYRMLMDFEQLQPGDSVIQNASNSGVGQAVIQIAAALGLR
  TINVVRDRPDIQKLSDRLKSLGAEHVITEEELRRPEMKNFFKDMPQPRLALNCVGGKSSTELLRQLARGG
  TMVTYGGMAKQPVVASVSLLIFKDLKLRGFWLSQWKKDHSPDQFKELILTLCDLIRRGQLTAPACSQVPL
  QDYQSALEASMKPFISSKQILTM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016011
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016011.3
• RefSeq Size: 2539 bp
• RefSeq ORF: 1122 bp
• Locus ID: 51102
• UniProt ID: Q9BV79
• Cytogenetics: 1p35.3
• Domains: ADH_zinc_N
• Protein Families: Druggable Genome
• Protein Pathways: Fatty acid elongation in mitochondria, Metabolic pathways
• MW: 40.4 kDa
• Gene Summary: The protein encoded by this gene is an oxidoreductase that catalyzes the last step in mitochondrial fatty acid synthesis. Defects in this gene are a cause of childhood-onset dystonia and optic atrophy. [provided by RefSeq, Mar 2017]