PPIG (NM_004792) Human Tagged ORF Clone

CAT#: RC224587

PPIG (Myc-DDK-tagged)-Human peptidylprolyl isomerase G (cyclophilin G) (PPIG)

Product Images

Circular map for RC224587

• 

  Western blot validation of overexpression lysate (Cat# LY417745) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224587 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPIG
• Synonyms: CARS-Cyp; CYP; SCAF10; SRCyp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224587 representing NM_004792
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAATAAAGGTTCAACGTCCTCGATGTTTTTTTGACATTGCCATTAACAATCAACCTGCTGGAAGAG
  TTGTCTTTGAATTATTTTCTGATGTGTGCCCCAAAACATGCGAGAACTTTCGTTGTCTTTGTACAGGTGA
  AAAGGGGACCGGGAAATCAACTCAGAAACCATTACATTATAAGAGTTGTCTCTTTCACAGAGTTGTCAAG
  GATTTTATGGTTCAAGGTGGTGACTTCAGTGAAGGAAATGGACGAGGAGGGGAATCTATCTATGGAGGAT
  TTTTTGAAGACGAGAGTTTCGCTGTTAAACACAACAAAGAATTTCTCTTGTCAATGGCCAACAGAGGGAA
  GGATACAAATGGTTCACAGTTCTTCATAACAACGAAACCAACTCCTCATTTAGATGGGCATCATGTTGTT
  TTTGGACAAGTAATCTCTGGTCAAGAAGTTGTAAGAGAGATTGAAAACCAGAAAACAGATGCAGCTAGCA
  AACCGTTTGCGGAGGTACGGATACTCAGTTGTGGAGAGCTGATTCCCAAATCTAAAGTTAAGAAAGAAGA
  AAAGAAAAGGCATAAATCATCATCATCTTCCTCCTCCTCATCTAGTGACTCAGATAGCTCAAGTGATTCT
  CAGTCCTCTTCTGATTCCTCTGATTCCGAAAGTGCTACTGAAGAGAAATCAAAGAAAAGAAAAAAGAAAC
  ATCGGAAAAATTCCCGAAAACACAAGAAAGAAAAGAAAAAGCGAAAGAAAAGCAAGAAGAGTGCATCTAG
  TGAGAGTGAAGCTGAAAATCTTGAAGCACAACCCCAGTCTACTGTCCGTCCAGAAGAGATCCCTCCTATA
  CCTGAAAATAGATTCCTAATGAGAAAAAGTCCTCCTAAAGCTGATGAGAAGGAAAGGAAAAACAGAGAGA
  GAGAAAGGGAAAGAGAGTGTAATCCACCTAACTCCCAGCCTGCTTCATACCAGAGACGACTTTTAGTTAC
  TAGATCTGGCAGGAAAATTAAAGGAAGAGGACCAAGGCGTTATCGAACTCCTTCCAGATCCAGATCAAGG
  GATCGTTTCAGACGTAGTGAGACTCCTCCACATTGGAGGCAAGAGATGCAGAGAGCTCAAAGAATGAGGG
  TATCAAGTGGTGAAAGATGGATCAAGGGGGATAAGAGTGAGTTGAATGAAATAAAAGAAAATCAGAGAAG
  TCCAGTTAGAGTAAAAGAGAGAAAAATAACAGATCACAGGAATGTATCTGAGAGTCCAAACAGAAAAAAT
  GAAAAGGAGAAGAAAGTTAAAGACCATAAATCTAACAGCAAAGAGAGAGACATCAGAAGAAATTCAGAAA
  AAGATGACAAGTATAAAAACAAAGTGAAGAAAAGGGCCAAATCTAAAAGTAGGAGTAAGAGCAAAGAGAA
  ATCAAAGAGTAAAGAAAGAGATTCAAAACATAATAGAAATGAAGAAAAGAGGATGAGGTCAAGGAGTAAA
  GGAAGGGATCATGAAAATGTTAAAGAAAAAGAAAAGCAGTCTGATTCTAAAGGAAAAGATCAGGAAAGGA
  GTAGAAGTAAAGAGAAGTCTAAACAGTTAGAATCAAAGAGTAATGAGCATGATCACAGTAAAAGTAAGGA
  AAAGGATAGACGCGCACAATCCAGGAGTAGAGAATGTGATATAACTAAAGGTAAACACAGTTATAATAGC
  AGAACAAGAGAACGAAGCAGAAGTAGGGACAGAAGCAGAAGAGTGCGATCAAGAACCCATGACAGAGATC
  GCAGCAGAAGCAAGGAGTACCATAGATACAGAGAACAGGAATACAGGAGAAGAGGACGGTCACGAAGCCG
  AGAGAGAAGAACACCACCAGGAAGATCAAGAAGTAAAGATAGGAGGAGAAGGAGGAGAGACTCACGGAGC
  TCAGAGAGAGAAGAAAGTCAAAGCAGAAACAAAGACAAATACAGAAACCAAGAGAGTAAGAGCTCACACA
  GAAAAGAAAATTCTGAGAGTGAGAAAAGAATGTACTCTAAAAGTCGTGATCATAATAGCTCAAATAACAG
  CAGGGAAAAAAAGGCTGATAGAGATCAAAGTCCCTTCTCAAAAATAAAACAAAGCAGTCAGGACAATGAA
  TTAAAGTCCTCCATGTTGAAAAATAAGGAGGATGAGAAGATCAGATCCTCAGTGGAAAAAGAAAACCAAA
  AATCAAAAGGTCAAGAAAATGACCATGTACATGAAAAAAATAAAAAATTTGATCATGAATCAAGCCCTGG
  AACAGATGAAGACAAAAGCGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224587 representing NM_004792
  Red=Cloning site Green=Tags(s)
  
  MGIKVQRPRCFFDIAINNQPAGRVVFELFSDVCPKTCENFRCLCTGEKGTGKSTQKPLHYKSCLFHRVVK
  DFMVQGGDFSEGNGRGGESIYGGFFEDESFAVKHNKEFLLSMANRGKDTNGSQFFITTKPTPHLDGHHVV
  FGQVISGQEVVREIENQKTDAASKPFAEVRILSCGELIPKSKVKKEEKKRHKSSSSSSSSSSDSDSSSDS
  QSSSDSSDSESATEEKSKKRKKKHRKNSRKHKKEKKKRKKSKKSASSESEAENLEAQPQSTVRPEEIPPI
  PENRFLMRKSPPKADEKERKNRERERERECNPPNSQPASYQRRLLVTRSGRKIKGRGPRRYRTPSRSRSR
  DRFRRSETPPHWRQEMQRAQRMRVSSGERWIKGDKSELNEIKENQRSPVRVKERKITDHRNVSESPNRKN
  EKEKKVKDHKSNSKERDIRRNSEKDDKYKNKVKKRAKSKSRSKSKEKSKSKERDSKHNRNEEKRMRSRSK
  GRDHENVKEKEKQSDSKGKDQERSRSKEKSKQLESKSNEHDHSKSKEKDRRAQSRSRECDITKGKHSYNS
  RTRERSRSRDRSRRVRSRTHDRDRSRSKEYHRYREQEYRRRGRSRSRERRTPPGRSRSKDRRRRRRDSRS
  SEREESQSRNKDKYRNQESKSSHRKENSESEKRMYSKSRDHNSSNNSREKKADRDQSPFSKIKQSSQDNE
  LKSSMLKNKEDEKIRSSVEKENQKSKGQENDHVHEKNKKFDHESSPGTDEDKSG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004792
• ORF Size: 2262 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004792.3
• RefSeq Size: 2717 bp
• RefSeq ORF: 2265 bp
• Locus ID: 9360
• UniProt ID: Q13427
• Cytogenetics: 2q31.1
• Domains: pro_isomerase
• MW: 88.4 kDa
• Gene Summary: PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.[UniProtKB/Swiss-Prot Function]