PPP2R2B (NM_181676) Human Tagged ORF Clone

CAT#: RC224583

PPP2R2B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 4

Product Images

Circular map for RC224583

• 

  Western blot validation of overexpression lysate (Cat# LY405665) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224583 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPP2R2B
• Synonyms: B55BETA; PP2AB55BETA; PP2ABBETA; PP2APR55B; PP2APR55BETA; PR2AB55BETA; PR2ABBETA; PR2APR55BETA; PR52B; PR55-BETA; PR55BETA; SCA12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224583 representing NM_181676
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATGCTTCTCTCGTTACCTGCCTTACATCTTCAGACCTCCGAACACCATCCTTTCTTCCAGCTGCC
  ACACAGAAGCTGACATTATCTCTACGGTAGAATTCAACCACACGGGAGAATTACTAGCGACAGGGGACAA
  GGGGGGTCGGGTTGTAATATTTCAACGAGAGCAGGAGAGTAAAAATCAGGTTCATCGTAGGGGTGAATAC
  AATGTTTACAGCACATTCCAGAGCCATGAACCCGAGTTCGATTACCTGAAGAGTTTAGAAATAGAAGAAA
  AAATCAATAAAATAAGATGGCTCCCCCAGCAGAATGCAGCTTACTTTCTTCTGTCTACTAATGATAAAAC
  TGTGAAGCTGTGGAAAGTCAGCGAGCGTGATAAGAGGCCAGAAGGCTACAATCTGAAAGATGAGGAGGGC
  CGGCTCCGGGATCCTGCCACCATCACAACCCTGCGGGTGCCTGTCCTGAGACCCATGGACCTGATGGTGG
  AGGCCACCCCACGAAGAGTATTTGCCAACGCACACACATATCACATCAACTCCATATCTGTCAACAGCGA
  CTATGAAACCTACATGTCCGCTGATGACCTGAGGATTAACCTATGGAACTTTGAAATAACCAATCAAAGT
  TTTAATATTGTGGACATTAAGCCAGCCAACATGGAGGAGCTCACGGAGGTGATCACAGCAGCCGAGTTCC
  ACCCCCATCATTGCAACACCTTCGTGTACAGCAGCAGCAAAGGGACAATCCGGCTGTGTGACATGCGGGC
  ATCTGCCCTGTGTGACAGGCACACCAAATTTTTTGAAGAGCCGGAAGATCCAAGCAACAGATCATTTTTC
  TCTGAAATTATCTCTTCGATTTCGGATGTGAAGTTCAGCCACAGTGGGAGGTATATCATGACCAGGGACT
  ACTTGACCGTCAAAGTCTGGGATCTCAACATGGAAAACCGCCCCATCGAGACTTACCAGGTTCATGACTA
  CCTCCGCAGCAAGCTGTGTTCCCTCTATGAAAATGACTGCATTTTTGATAAATTTGAGTGTGTGTGGAAT
  GGGTCAGACAGTGTCATCATGACAGGCTCCTACAACAACTTCTTCAGGATGTTCGACAGAAACACCAAGC
  GTGATGTGACCCTTGAGGCTTCGAGGGAAAACAGCAAGCCCCGGGCTATCCTCAAACCCCGAAAAGTGTG
  TGTGGGGGGCAAGCGGAGAAAAGACGAGATCAGTGTCGACAGTCTGGACTTTAGCAAAAAGATCTTGCAT
  ACAGCTTGGCATCCTTCAGAAAATATTATAGCAGTGGCGGCTACAAATAACCTATATATATTCCAGGACA
  AGGTTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224583 representing NM_181676
  Red=Cloning site Green=Tags(s)
  
  MKCFSRYLPYIFRPPNTILSSSCHTEADIISTVEFNHTGELLATGDKGGRVVIFQREQESKNQVHRRGEY
  NVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAYFLLSTNDKTVKLWKVSERDKRPEGYNLKDEEG
  RLRDPATITTLRVPVLRPMDLMVEATPRRVFANAHTYHINSISVNSDYETYMSADDLRINLWNFEITNQS
  FNIVDIKPANMEELTEVITAAEFHPHHCNTFVYSSSKGTIRLCDMRASALCDRHTKFFEEPEDPSNRSFF
  SEIISSISDVKFSHSGRYIMTRDYLTVKVWDLNMENRPIETYQVHDYLRSKLCSLYENDCIFDKFECVWN
  GSDSVIMTGSYNNFFRMFDRNTKRDVTLEASRENSKPRAILKPRKVCVGGKRRKDEISVDSLDFSKKILH
  TAWHPSENIIAVAATNNLYIFQDKVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181676
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181676.2
• RefSeq Size: 1978 bp
• RefSeq ORF: 1341 bp
• Locus ID: 5521
• UniProt ID: Q00005
• Cytogenetics: 5q32
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Tight junction
• MW: 51.8 kDa
• Gene Summary: The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in this gene cause autosomal dominant spinocerebellar ataxia 12 (SCA12), a disease caused by degeneration of the cerebellum, sometimes involving the brainstem and spinal cord, and in resulting in poor coordination of speech and body movements. Multiple alternatively spliced variants, which encode different isoforms, have been identified for this gene. The 5' UTR of some of these variants includes a CAG trinucleotide repeat sequence (7-28 copies) that can be expanded to 55-78 copies in cases of SCA12. [provided by RefSeq, Jul 2016]