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CMH1 (MYH7) (NM_000257) Human Tagged ORF Clone
CAT#: RC224521
- TrueORF®
MYH7 (Myc-DDK-tagged)-Human myosin, heavy chain 7, cardiac muscle, beta (MYH7)
ORF Plasmid: tGFP
"NM_000257" in other vectors (2)
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Frequently bought together (4)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
USD 198.00
Other products for "CMH1"
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | CMH1 |
| Synonyms | CMD1S; CMH1; MPD1; MYHCB; SPMD; SPMM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224521 representing NM_000257
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGATTCGGAGATGGCAGTCTTTGGGGCTGCCGCCCCCTACCTGCGCAAGTCAGAGAAGGAGCGGC TAGAAGCGCAGACCAGGCCTTTTGACCTCAAGAAGGATGTCTTCGTGCCTGATGACAAACAGGAGTTTGT CAAGGCCAAGATCGTGTCTCGAGAGGGTGGCAAAGTCACTGCCGAGACCGAGTATGGCAAGACAGTGACC GTGAAGGAGGACCAGGTGATGCAGCAGAACCCACCCAAGTTCGACAAAATCGAGGACATGGCCATGCTGA CCTTCCTGCATGAGCCCGCGGTGCTCTACAACCTCAAGGATCGCTACGGCTCCTGGATGATCTACACCTA CTCGGGCCTCTTCTGTGTCACCGTCAACCCTTACAAGTGGCTGCCGGTGTACACTCCTGAGGTGGTGGCT GCCTACCGGGGCAAGAAGAGGAGCGAGGCCCCGCCCCACATCTTCTCCATCTCCGACAACGCCTATCAGT ACATGCTGACAGACAGAGAAAACCAGTCCATCCTGATCACCGGAGAATCCGGAGCAGGGAAGACAGTCAA CACCAAGAGGGTCATCCAGTACTTTGCTGTTATTGCAGCCATTGGGGACCGCAGCAAGAAGGACCAGAGC CCGGGCAAGGGCACCCTGGAGGACCAGATCATCCAGGCCAACCCTGCTCTGGAGGCCTTTGGCAATGCCA AGACCGTCCGGAACGACAACTCCTCCCGCTTCGGGAAATTCATTCGAATTCATTTTGGGGCAACAGGAAA GTTGGCATCTGCAGACATAGAGACCTATCTTCTGGAAAAATCCAGAGTTATTTTCCAGCTGAAAGCAGAG AGAGATTATCACATTTTCTACCAAATCCTGTCTAACAAAAAGCCTGAGCTGCTGGACATGCTGCTGATCA CCAACAACCCCTACGATTATGCATTCATCTCCCAAGGAGAGACCACCGTGGCCTCCATTGATGACGCTGA GGAGCTCATGGCCACTGATAACGCTTTTGATGTGCTGGGCTTCACTTCAGAGGAGAAAAACTCCATGTAT AAGCTGACAGGCGCCATCATGCACTTTGGAAACATGAAGTTCAAGCTGAAGCAGCGGGAGGAGCAGGCGG AGCCAGACGGCACTGAAGAGGCTGACAAGTCTGCCTACCTCATGGGGCTGAACTCAGCCGACCTGCTCAA GGGGCTGTGCCACCCTCGGGTGAAAGTGGGCAATGAGTACGTCACCAAGGGGCAGAATGTCCAGCAGGTG ATATATGCCACTGGGGCACTGGCCAAGGCAGTGTATGAGAGGATGTTCAACTGGATGGTGACGCGCATCA ATGCCACCCTGGAGACCAAGCAGCCACGCCAGTACTTCATAGGAGTCCTGGACATCGCTGGCTTCGAGAT CTTCGATTTCAACAGCTTTGAGCAGCTCTGCATCAACTTCACCAACGAGAAGCTGCAGCAGTTCTTCAAC CACCACATGTTTGTGCTGGAGCAGGAGGAGTACAAGAAGGAGGGCATCGAGTGGACATTCATTGACTTTG GCATGGACCTGCAGGCCTGCATTGACCTCATCGAGAAGCCCATGGGCATCATGTCCATCCTGGAAGAGGA GTGCATGTTCCCCAAGGCCACCGACATGACCTTCAAGGCCAAGCTGTTTGACAACCACCTGGGCAAATCC GCCAACTTCCAGAAGCCACGCAATATCAAGGGGAAGCCTGAAGCCCACTTCTCCCTGATCCACTATGCCG GCATCGTGGACTACAACATCATTGGCTGGCTGCAGAAGAACAAGGATCCTCTCAATGAGACTGTCGTGGG CTTGTATCAGAAGTCTTCCCTCAAGCTGCTCAGCACCCTGTTTGCCAACTATGCTGGGGCTGATGCGCCT ATTGAGAAGGGCAAAGGCAAGGCCAAGAAAGGCTCGTCCTTTCAGACTGTGTCAGCTCTGCACAGGGAAA ATCTGAACAAGCTGATGACCAACTTGCGCTCCACCCATCCCCACTTTGTACGTTGTATCATCCCTAATGA GACAAAGTCTCCAGGGGTGATGGACAACCCCCTGGTCATGCACCAGCTGCGCTGCAATGGTGTGCTGGAG GGCATCCGCATCTGCAGGAAAGGCTTCCCCAACCGCATCCTCTACGGGGACTTCCGGCAGAGGTATCGCA TCCTGAACCCAGCGGCCATCCCTGAGGGACAGTTCATTGATAGCAGGAAGGGGGCAGAGAAGCTGCTCAG CTCCCTGGACATTGATCACAACCAGTACAAGTTTGGCCACACCAAGGTGTTCTTCAAGGCCGGGCTGCTG GGGCTGCTGGAGGAAATGAGGGACGAGAGGCTGAGCCGCATCATCACGCGTATCCAGGCCCAGTCCCGAG GTGTGCTCGCCAGAATGGAGTACAAAAAGCTGCTGGAACGTAGAGACTCCCTGCTGGTAATCCAGTGGAA CATTCGGGCCTTCATGGGGGTCAAGAATTGGCCCTGGATGAAGCTCTACTTCAAGATCAAGCCGCTGCTG AAGAGTGCAGAAAGAGAGAAGGAGATGGCCTCCATGAAGGAGGAGTTCACACGCCTCAAAGAGGCGCTAG AGAAGTCCGAGGCTCGCCGCAAGGAGCTGGAGGAGAAGATGGTGTCCCTGCTGCAGGAGAAGAATGACCT GCAGCTCCAAGTGCAGGCGGAACAAGACAACCTGGCAGATGCTGAGGAGCGCTGTGATCAGCTGATCAAA AACAAGATTCAGCTGGAGGCCAAGGTGAAGGAGATGAACGAGAGGCTGGAGGATGAGGAGGAGATGAATG CTGAGCTCACTGCCAAGAAGCGCAAGCTGGAAGATGAGTGCTCAGAGCTCAAAAGGGACATCGATGATCT GGAGCTGACACTGGCCAAAGTGGAGAAGGAGAAACACGCAACAGAGAACAAGGTGAAAAACCTGACAGAG GAGATGGCTGGGCTGGATGAGATCATTGCCAAGCTGACCAAGGAGAAGAAAGCTCTGCAAGAGGCCCACC AACAGGCTCTGGATGACCTTCAGGCCGAGGAGGACAAGGTCAACACCCTGACTAAGGCCAAAGTCAAGCT GGAGCAGCAAGTGGATGATCTGGAAGGATCCCTGGAGCAAGAGAAGAAGGTGCGCATGGACCTGGAGCGA GCGAAGCGGAAGCTGGAGGGCGACCTGAAGCTGACCCAGGAGAGCATCATGGACCTGGAGAATGACAAGC AGCAGCTGGATGAGCGGCTGAAAAAAAAAGACTTTGAGCTGAATGCTCTCAACGCAAGGATTGAGGATGA ACAGGCCCTCGGCAGCCAGCTGCAGAAGAAGCTCAAGGAGCTTCAGGCACGCATCGAGGAGCTGGAGGAG GAGCTGGAGGCCGAGCGCACCGCCAGGGCTAAGGTGGAGAAGCTGCGCTCAGACCTGTCTCGGGAGCTGG AGGAGATCAGCGAGCGGCTGGAAGAGGCCGGCGGGGCCACGTCCGTGCAGATCGAGATGAACAAGAAGCG CGAGGCCGAGTTCCAGAAGATGCGGCGGGACCTGGAGGAGGCCACGCTGCAGCACGAGGCCACTGCCGCG GCCCTGCGCAAGAAGCACGCCGACAGCGTGGCCGAGCTGGGCGAGCAGATCGACAACCTGCAGCGGGTGA AGCAGAAGCTGGAGAAGGAGAAGAGCGAGTTCAAGCTGGAGCTGGATGACGTCACCTCCAACATGGAGCA GATCATCAAGGCCAAGGCTAACCTGGAGAAGATGTGCCGGACCTTGGAAGACCAGATGAATGAGCACCGG AGCAAGGCGGAGGAGACCCAGCGTTCTGTCAACGACCTCACCAGCCAGCGGGCCAAGTTGCAAACCGAGA ATGGTGAGCTGTCCCGGCAGCTGGATGAGAAGGAGGCACTGATCTCCCAGCTGACCCGAGGCAAGCTCAC CTACACCCAGCAGCTGGAGGACCTCAAGAGGCAGCTGGAGGAGGAGGTTAAGGCGAAGAACGCCCTGGCC CACGCACTGCAGTCGGCCCGGCATGACTGCGACCTGCTGCGGGAGCAGTACGAGGAGGAGACGGAGGCCA AGGCCGAGCTGCAGCGCGTCCTTTCCAAGGCCAACTCGGAGGTGGCCCAGTGGAGGACCAAGTATGAGAC GGACGCCATTCAGCGGACTGAGGAGCTCGAGGAGGCCAAGAAGAAGCTGGCCCAGCGGCTGCAGGAAGCT GAGGAGGCCGTGGAGGCTGTTAATGCCAAGTGCTCCTCGCTGGAGAAGACCAAGCACCGGCTACAGAATG AGATCGAGGACTTGATGGTGGACGTAGAGCGCTCCAATGCTGCTGCTGCAGCCCTGGACAAGAAGCAGAG GAACTTCGACAAGATCCTGGCCGAGTGGAAGCAGAAGTATGAGGAGTCGCAGTCGGAGCTGGAGTCCTCG CAGAAGGAGGCTCGCTCCCTCAGCACAGAGCTCTTCAAACTCAAGAACGCCTATGAGGAGTCCCTGGAAC ATCTGGAGACCTTCAAGCGGGAGAACAAAAACCTGCAGGAGGAGATCTCCGACTTGACTGAGCAGTTGGG TTCCAGCGGAAAGACTATCCATGAGCTGGAGAAGGTCCGAAAGCAGCTGGAGGCCGAGAAGATGGAGCTG CAGTCAGCCCTGGAGGAGGCCGAGGCCTCCCTGGAGCACGAGGAGGGCAAGATCCTCCGGGCCCAGCTGG AGTTCAACCAGATCAAGGCAGAGATCGAGCGGAAGCTGGCAGAGAAGGACGAGGAGATGGAACAGGCCAA GCGCAACCACCTGCGGGTGGTGGACTCGCTGCAGACCTCCCTGGACGCAGAGACACGCAGCCGCAACGAG GCCCTGAGGGTGAAGAAGAAGATGGAAGGAGACCTCAATGAGATGGAGATCCAGCTCAGCCACGCCAACC GCATGGCCGCCGAGGCCCAGAAGCAAGTCAAGAGCCTCCAGAGCTTGTTGAAGGACACCCAGATTCAGCT GGACGATGCAGTCCGTGCCAACGACGACCTGAAGGAGAACATCGCCATCGTGGAGCGGCGCAACAACCTG CTGCAGGCTGAGCTGGAGGAGTTGCGTGCCGTGGTGGAGCAGACAGAGCGGTCCCGGAAGCTGGCGGAGC AGGAGCTGATTGAGACTAGTGAGCGGGTGCAGCTGCTGCATTCCCAGAACACCAGCCTCATCAACCAGAA GAAGAAGATGGATGCTGACCTGTCCCAGCTCCAGACTGAAGTGGAGGAGGCAGTGCAGGAGTGCAGGAAT GCTGAGGAGAAGGCCAAGAAGGCCATCACGGATGCCGCCATGATGGCAGAGGAGCTGAAGAAGGAGCAGG ACACCAGCGCCCACCTGGAGCGCATGAAGAAGAACATGGAACAGACCATTAAGGACCTGCAGCACCGGCT GGACGAAGCCGAGCAGATCGCCCTCAAGGGCGGCAAGAAGCAGCTGCAGAAGCTGGAAGCGCGGGTGCGG GAGCTGGAGAATGAGCTGGAGGCCGAGCAGAAGCGCAACGCAGAGTCGGTGAAGGGCATGAGGAAGAGCG AGCGGCGCATCAAGGAGCTCACCTACCAGACGGAGGAGGACAGGAAAAACCTGCTGCGGCTGCAGGACCT GGTAGACAAGCTGCAGCTAAAGGTCAAGGCCTACAAGCGCCAGGCCGAGGAGGCGGAGGAGCAAGCCAAC ACCAACCTGTCCAAGTTCCGCAAGGTGCAGCACGAGCTGGATGAGGCAGAGGAGCGGGCGGACATCGCCG AGTCCCAGGTCAACAAGCTGCGGGCCAAGAGCCGTGACATTGGCACGAAGGGCTTGAATGAGGAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC224521 representing NM_000257
Red=Cloning site Green=Tags(s) MGDSEMAVFGAAAPYLRKSEKERLEAQTRPFDLKKDVFVPDDKQEFVKAKIVSREGGKVTAETEYGKTVT VKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKDRYGSWMIYTYSGLFCVTVNPYKWLPVYTPEVVA AYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQS PGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAE RDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMY KLTGAIMHFGNMKFKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQV IYATGALAKAVYERMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFN HHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKS ANFQKPRNIKGKPEAHFSLIHYAGIVDYNIIGWLQKNKDPLNETVVGLYQKSSLKLLSTLFANYAGADAP IEKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLE GIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYKFGHTKVFFKAGLL GLLEEMRDERLSRIITRIQAQSRGVLARMEYKKLLERRDSLLVIQWNIRAFMGVKNWPWMKLYFKIKPLL KSAEREKEMASMKEEFTRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIK NKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTE EMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLER AKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEE ELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAA ALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHR SKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALA HALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQEA EEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESS QKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKMEL QSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNE ALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNL LQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRN AEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVR ELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQAN TNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGTKGLNEE SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000257 |
| ORF Size | 5805 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000257.4 |
| RefSeq Size | 6044 bp |
| RefSeq ORF | 5808 bp |
| Locus ID | 4625 |
| UniProt ID | P12883 |
| Cytogenetics | 14q11.2 |
| Domains | IQ, myosin_head, Myosin_tail, M, Myosin_N |
| Protein Pathways | Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), Tight junction, Viral myocarditis |
| MW | 223.5 kDa |
| Gene Summary | Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq, Jul 2008] |
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