ZNF749 (NM_001023561) Human Tagged ORF Clone

CAT#: RC224405

ZNF749 (Myc-DDK-tagged)-Human zinc finger protein 749 (ZNF749)

Product Images

Circular map for RC224405

• 

  Western blot validation of overexpression lysate (Cat# LY422604) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224405 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF749
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224405 representing NM_001023561
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTGACCGAGGATTGTATGGTCTTTGAGGATGTGGCCATATATTTCTCCCAAGAGGAATGGGGGA
  TCCTTAATGATGCTCAGAGACACCTGCACAGCAATGTGATGTTGGAGAACTTTGCGCTTTTGTCATCAGT
  AGGTTGTTGGCATGGAGCCAAGGATGAGGAGGTACCTTCCAAGCAGTGTGTTTCTGTAAGAGTGTTACAG
  GTCACAATTCCAAAGCCAGCTTTGTCCACCCTGAAGGCCCAGCCCTGCAAGATGTGTAGCTCAATTCTGA
  AGGACATTCTGCACCTGGCTGAGCACGATGGAACACACCCTGAGCAAGGGCTGTACACATGTGCAGCAGA
  GCATGACCTGCACCAAAAGGAGCAGATTAGAGAGAAGCTCACCAGAAGTGATGAGTGGAGGCCTTCATTT
  GTGAACCACAGTGCTCACGTGGGAGAGAGGAACTTCACATGCACGCAGGGTGGCAAGGATTTTACTGCCA
  GCTCAGACCTTCTCCAGCAACAGGTCTTAAACAGTGGGTGGAAGCTGTACAGGGATACCCAGGATGGGGA
  AGCCTTTCAAGGTGAACAGAATGATTTCAACTCCAGCCAAGGTGGGAAAGACTTTTGCCACCAACATGGG
  CTGTTTGAGCACCAAAAAACCCATAATGGGGAGAGGCCTTATGAGTTCAGTGAATGTGGGGAATTGTTTA
  GGTACAACTCCAACCTTATTAAATATCAGCAAAATCATGCTGGAGAAAGGCCTTATGAGGGCACTGAATA
  TGGAAAGACCTTTATTAGAAAGTCCAACCTAGTTCAGCACCAGAAAATTCACAGTGAAGGCTTTCTTTCA
  AAAAGGTCTGACCCCATTGAACATCAGGAGATTCTCAGTAGACCAACACCTTATGAATGCACCCAGTGTG
  GGAAGGCCTTTCTTACACAGGCTCATCTGGTTGGTCACCAGAAAACCCATACTGGAGAACAGCCCTATGA
  ATGCAACAAGTGTGGGAAGTTTTTTATGTATAACTCCAAACTCATCAGACATCAGAAAGTTCACACTGGG
  GAGAGGCGTTACGAGTGCAGTGAATGTGGGAAATTGTTTATGGACAGCTTCACACTCGGTAGACATCAGA
  GAGTTCATACTGGAGAAAGGCCTTTTGAATGCAGCATATGTGGAAAATTCTTTAGTCACCGCTCCACACT
  CAATATGCACCAGAGAGTTCATGCTGGCAAAAGGCTTTATAAGTGTAGCGAATGTGGGAAAGCCTTTAGC
  CTCAAACATAATGTTGTTCAGCATCTGAAAATTCATACTGGAGAACGGCCTTATGAGTGCACTGAATGTG
  AGAAGGCCTTTGTTAGAAAGTCCCACCTAGTTCAGCACCAGAAAATCCACACTGATGCATTTTCAAAAAG
  GTCTGACCTCATTCAACACAAGAGGATTGACATTAGGCCAAGGCCTTATACATGCAGTGAATGTGGGAAG
  GCCTTCCTTACACAGGCTCATCTGGTTGGTCACCAGAAAATCCATACTGGAGAACGGCCTTATGAATGCA
  CTCAATGTGCGAAGGCCTTTGTTAGAAAGTCCCACCTAGTTCAGCATGAGAAAATCCACACTGATGCATT
  TTCAAAAAGGTCTGACCTCATTCAACACAAGAGGATTGACCTCAGGCCAAGGCCTTATGTGTGTAGTGAA
  TGTGGGAAGGCCTTCCTTACACAGGCTCATCTAGATGGTCACCAGAAAATCCAGACTGGAGAACGGCGTT
  ATGAATGCAATGAATGTGGGAAATTCTTTTTGGACAGCTACAAACTTGTTATTCATCAGAGAATTCACAC
  TGGAGAAAAGCCTTATAAATGCAGCAAATGTGGGAAATTCTTTAGATATCGCTGTACACTGAGTAGACAT
  CAGAAAGTTCACACTGGAGAAAGACCTTATGAGTGTAGTGAATGTGGGAAATTTTTTAGAGATAGCTACA
  AACTCATTATTCATCAGAGAGTTCATACTGGAGAAAAGCCTTATGAATGCAGCAACTGTGGGAAGTTTCT
  TAGATACCGCTCTACATTCATTAAACATCATAAAGTTTGCACTGGGGAGAAGCCTCATGAGTGCAGTAAA
  TGTAGGGAATTGTTTAGGACTAAATCGAGCCTTATTATACATCAGCAGTCTCACACTGGAGAAAGTCCTT
  TTAAGTTAAGGGAATGTGGGAAAGACTTCAACAAATGTAATACTGGTCAGCGCCAAAAAACTCACACTGG
  AGAAAGGTCTTATGAGTGTGGTGAATCCAGCAAAGTGTTTAAATACAACTCCAGCCTCATTAAACATCAG
  ATAATTCATACTGGAAAAAGGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224405 representing NM_001023561
  Red=Cloning site Green=Tags(s)
  
  MNLTEDCMVFEDVAIYFSQEEWGILNDAQRHLHSNVMLENFALLSSVGCWHGAKDEEVPSKQCVSVRVLQ
  VTIPKPALSTLKAQPCKMCSSILKDILHLAEHDGTHPEQGLYTCAAEHDLHQKEQIREKLTRSDEWRPSF
  VNHSAHVGERNFTCTQGGKDFTASSDLLQQQVLNSGWKLYRDTQDGEAFQGEQNDFNSSQGGKDFCHQHG
  LFEHQKTHNGERPYEFSECGELFRYNSNLIKYQQNHAGERPYEGTEYGKTFIRKSNLVQHQKIHSEGFLS
  KRSDPIEHQEILSRPTPYECTQCGKAFLTQAHLVGHQKTHTGEQPYECNKCGKFFMYNSKLIRHQKVHTG
  ERRYECSECGKLFMDSFTLGRHQRVHTGERPFECSICGKFFSHRSTLNMHQRVHAGKRLYKCSECGKAFS
  LKHNVVQHLKIHTGERPYECTECEKAFVRKSHLVQHQKIHTDAFSKRSDLIQHKRIDIRPRPYTCSECGK
  AFLTQAHLVGHQKIHTGERPYECTQCAKAFVRKSHLVQHEKIHTDAFSKRSDLIQHKRIDLRPRPYVCSE
  CGKAFLTQAHLDGHQKIQTGERRYECNECGKFFLDSYKLVIHQRIHTGEKPYKCSKCGKFFRYRCTLSRH
  QKVHTGERPYECSECGKFFRDSYKLIIHQRVHTGEKPYECSNCGKFLRYRSTFIKHHKVCTGEKPHECSK
  CRELFRTKSSLIIHQQSHTGESPFKLRECGKDFNKCNTGQRQKTHTGERSYECGESSKVFKYNSSLIKHQ
  IIHTGKRP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001023561
• ORF Size: 2334 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001023561.4
• RefSeq Size: 2929 bp
• RefSeq ORF: 2337 bp
• Locus ID: 388567
• UniProt ID: O43361
• Cytogenetics: 19q13.43
• MW: 90.2 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]