ZNF569 (NM_152484) Human Tagged ORF Clone

CAT#: RC224370

ZNF569 (Myc-DDK-tagged)-Human zinc finger protein 569 (ZNF569)

Product Images

Circular map for RC224370

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF569
• Synonyms: ZAP1; Zfp74; ZNF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224370 representing NM_152484
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGAGTCCCAGGGAACAGTAACATTCAAAGATGTGGCTATCGACTTCACTCAGGAGGAGTGGAAGA
  GATTGGATCCTGCTCAGAGAAAACTGTACCGGAATGTGATGCTAGAAAACTATAACAACTTAATCACAGT
  AGGCTATCCGTTCACCAAACCTGATGTGATTTTCAAATTGGAGCAAGAAGAAGAACCATGGGTGATGGAG
  GAAGAAGTATTAAGGAGACACTGGCAAGGAGAAATATGGGGAGTTGATGAGCATCAGAAAAACCAGGACA
  GACTTTTGAGACAAGTTGAAGTTAAATTCCAGAAAACACTGACTGAAGAAAAAGGCAATGAATGTCAAAA
  GAAATTTGCAAATGTATTTCCTCTGAACTCTGATTTTTTCCCTTCCAGACACAATCTCTATGAGTATGAC
  TTATTTGGAAAGTGTTTAGAACATAATTTTGACTGTCATAATAATGTGAAATGCCTTATGAGAAAGGAGC
  ATTGTGAATATAATGAACCTGTGAAATCATATGGTAATAGCTCATCCCATTTTGTCATTACCCCCTTTAA
  GTGTAATCATTGTGGAAAAGGCTTCAATCAGACTTTGGACCTCATCAGACATCTGAGAATTCATACTGGA
  GAGAAGCCCTATGAATGTAGTAACTGTAGAAAAGCCTTCAGTCACAAGGAAAAACTTATTAAACATTATA
  AAATTCACAGTAGGGAGCAGTCTTACAAATGTAATGAATGTGGTAAAGCTTTCATTAAAATGTCAAATCT
  CATTAGACATCAAAGAATTCATACTGGAGAGAAGCCCTATGCATGTAAGGAATGTGAGAAGTCCTTCAGC
  CAGAAATCAAATCTTATTGATCATGAAAAAATTCATACTGGAGAGAAACCTTATGAATGTAATGAGTGTG
  GAAAAGCATTCAGCCAGAAGCAAAGCCTCATTGCACATCAGAAAGTTCATACTGGGGAGAAACCTTATGC
  ATGTAATGAATGTGGTAAAGCCTTCCCTCGAATTGCATCCCTTGCTCTTCATATGAGAAGTCATACAGGA
  GAAAAACCTTATAAATGTGATAAATGTGGTAAAGCCTTCTCTCAGTTTTCCATGCTTATTATACATGTTA
  GAATTCATACAGGTGAAAAACCCTATGAATGTAATGAGTGTGGAAAAGCCTTCTCTCAAAGCTCAGCCCT
  TACTGTACATATGAGAAGTCACACTGGTGAGAAACCCTATGAATGTAAGGAATGCAGAAAAGCCTTCAGC
  CACAAGAAAAACTTCATTACACACCAGAAAATTCATACTAGAGAGAAACCTTATGAGTGTAATGAATGTG
  GGAAAGCTTTTATACAGATGTCAAATCTTGTTAGACACCAGAGAATTCATACTGGGGAAAAACCCTATAT
  ATGTAAGGAATGTGGGAAAGCCTTTAGCCAGAAATCAAATCTCATTGCTCATGAAAAAATTCATTCTGGA
  GAGAAACCCTATGAATGCAATGAATGTGGTAAAGCCTTCAGCCAAAAGCAAAACTTCATTACACATCAAA
  AAGTTCATACTGGAGAGAAACCTTATGATTGTAATGAATGTGGTAAAGCCTTCTCTCAAATTGCATCCCT
  TACCCTTCATTTGAGAAGTCATACAGGGGAAAAGCCTTATGAATGTGATAAATGTGGTAAAGCCTTCTCT
  CAGTGCTCACTGCTTAATTTACATATGAGAAGTCACACAGGTGAGAAGCCCTATGTATGTAATGAATGTG
  GGAAAGCCTTCTCTCAAAGAACTTCCCTTATTGTGCACATGAGAGGCCATACAGGTGAAAAACCCTATGA
  ATGTAATAAATGTGGAAAAGCCTTCTCCCAAAGCTCATCCCTTACTATACATATACGAGGACATACAGGT
  GAGAAACCCTTCGACTGTAGTAAATGTGGAAAAGCCTTCTCTCAAATCTCATCTCTTACCCTTCATATGA
  GAAAACATACAGGTGAGAAGCCCTATCACTGTATTGAGTGTGGCAAGGCTTTCAGCCAAAAGTCGCACCT
  TGTTAGACACCAGAGAATTCATACTCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224370 representing NM_152484
  Red=Cloning site Green=Tags(s)
  
  MTESQGTVTFKDVAIDFTQEEWKRLDPAQRKLYRNVMLENYNNLITVGYPFTKPDVIFKLEQEEEPWVME
  EEVLRRHWQGEIWGVDEHQKNQDRLLRQVEVKFQKTLTEEKGNECQKKFANVFPLNSDFFPSRHNLYEYD
  LFGKCLEHNFDCHNNVKCLMRKEHCEYNEPVKSYGNSSSHFVITPFKCNHCGKGFNQTLDLIRHLRIHTG
  EKPYECSNCRKAFSHKEKLIKHYKIHSREQSYKCNECGKAFIKMSNLIRHQRIHTGEKPYACKECEKSFS
  QKSNLIDHEKIHTGEKPYECNECGKAFSQKQSLIAHQKVHTGEKPYACNECGKAFPRIASLALHMRSHTG
  EKPYKCDKCGKAFSQFSMLIIHVRIHTGEKPYECNECGKAFSQSSALTVHMRSHTGEKPYECKECRKAFS
  HKKNFITHQKIHTREKPYECNECGKAFIQMSNLVRHQRIHTGEKPYICKECGKAFSQKSNLIAHEKIHSG
  EKPYECNECGKAFSQKQNFITHQKVHTGEKPYDCNECGKAFSQIASLTLHLRSHTGEKPYECDKCGKAFS
  QCSLLNLHMRSHTGEKPYVCNECGKAFSQRTSLIVHMRGHTGEKPYECNKCGKAFSQSSSLTIHIRGHTG
  EKPFDCSKCGKAFSQISSLTLHMRKHTGEKPYHCIECGKAFSQKSHLVRHQRIHTH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152484
• ORF Size: 2058 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152484.3
• RefSeq Size: 4071 bp
• RefSeq ORF: 2061 bp
• Locus ID: 148266
• UniProt ID: Q5MCW4
• Cytogenetics: 19q13.12
• Protein Families: Transcription Factors
• MW: 79.4 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]