SMOC1 (NM_022137) Human Tagged ORF Clone

CAT#: RC224279

SMOC1 (Myc-DDK-tagged)-Human SPARC related modular calcium binding 1 (SMOC1), transcript variant 2

Product Images

Circular map for RC224279

• 

  Western blot validation of overexpression lysate (Cat# LY411742) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224279 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SMOC1
• Synonyms: OAS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224279 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCGCGCGCTGCGCCCGCCTGCTCACGCCCCACTTGCTGCTGGTGTTGGTGCAGCTGTCCCCTG
  CTCGCGGCCACCGCACCACAGGCCCCAGGTTTCTAATAAGTGACCGTGACCCACAGTGCAACCTCCACTG
  CTCCAGGACTCAACCCAAACCCATCTGTGCCTCTGATGGCAGGTCCTACGAGTCCATGTGTGAGTACCAG
  CGAGCCAAGTGCCGAGACCCGACCCTGGGCGTGGTGCATCGAGGTAGATGCAAAGATGCTGGCCAGAGCA
  AGTGTCGCCTGGAGCGGGCTCAAGCCCTGGAGCAAGCCAAGAAGCCTCAGGAAGCTGTGTTTGTCCCAGA
  GTGTGGCGAGGATGGCTCCTTTACCCAGGTGCAGTGCCATACTTACACTGGGTACTGCTGGTGTGTCACC
  CCGGATGGGAAGCCCATCAGTGGCTCTTCTGTGCAGAATAAAACTCCTGTATGTTCAGGTTCAGTCACCG
  ACAAGCCCTTGAGCCAGGGTAACTCAGGAAGGAAAGATGACGGGTCTAAGCCGACACCCACGATGGAGAC
  CCAGCCGGTGTTCGATGGAGATGAAATCACAGCCCCAACTCTATGGATTAAACACTTGGTGATCAAGGAC
  TCCAAACTGAACAACACCAACATAAGAAATTCAGAGAAAGTCTATTCGTGTGACCAGGAGAGGCAGAGTG
  CCCTGGAAGAGGCCCAGCAGAATCCCCGTGAGGGTATTGTCATCCCTGAATGTGCCCCTGGGGGACTCTA
  TAAGCCAGTGCAATGCCACCAGTCCACTGGCTACTGCTGGTGTGTGCTGGTGGACACAGGGCGCCCGCTG
  CCTGGGACCTCCACACGCTACGTGATGCCCAGTTGTGAGAGCGACGCCAGGGCCAAGACTACAGAGGCGG
  ATGACCCCTTCAAGGACAGGGAGCTACCAGGCTGTCCAGAAGGGAAGAAAATGGAGTTTATCACCAGCCT
  ACTGGATGCTCTCACCACTGACATGGTTCAGGCCATTAACTCAGCAGCGCCCACTGGAGGTGGGAGGTTC
  TCAGAGCCAGACCCCAGCCACACCCTGGAGGAGCGGGTAGTGCACTGGTATTTCAGCCAGCTGGACAGCA
  ATAGCAGCAACGACATTAACAAGCGGGAGATGAAGCCCTTCAAGCGCTACGTGAAGAAGAAAGCCAAGCC
  CAAGAAATGTGCCCGGCGTTTCACCGACTACTGTGACCTGAACAAAGACAAGGTCATTTCACTGCCTGAG
  CTGAAGGGCTGCCTGGGTGTTAGCAAAGAAGTAGGACGCCTCGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224279 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLPARCARLLTPHLLLVLVQLSPARGHRTTGPRFLISDRDPQCNLHCSRTQPKPICASDGRSYESMCEYQ
  RAKCRDPTLGVVHRGRCKDAGQSKCRLERAQALEQAKKPQEAVFVPECGEDGSFTQVQCHTYTGYCWCVT
  PDGKPISGSSVQNKTPVCSGSVTDKPLSQGNSGRKDDGSKPTPTMETQPVFDGDEITAPTLWIKHLVIKD
  SKLNNTNIRNSEKVYSCDQERQSALEEAQQNPREGIVIPECAPGGLYKPVQCHQSTGYCWCVLVDTGRPL
  PGTSTRYVMPSCESDARAKTTEADDPFKDRELPGCPEGKKMEFITSLLDALTTDMVQAINSAAPTGGGRF
  SEPDPSHTLEERVVHWYFSQLDSNSSNDINKREMKPFKRYVKKKAKPKKCARRFTDYCDLNKDKVISLPE
  LKGCLGVSKEVGRLV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022137
• ORF Size: 1013 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022137.1, NM_022137.2, NM_022137.3, NM_022137.4, NM_022137.5, NP_071420.1
• RefSeq Size: 3713 bp
• RefSeq ORF: 1305 bp
• Locus ID: 64093
• UniProt ID: Q9H4F8
• Cytogenetics: 14q24.2
• Protein Families: Secreted Protein
• MW: 48.3 kDa
• Gene Summary: This gene encodes a multi-domain secreted protein that may have a critical role in ocular and limb development. Mutations in this gene are associated with microphthalmia and limb anomalies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]