FAN1 (NM_014967) Human Tagged ORF Clone

CAT#: RC224249

FAN1 (Myc-DDK-tagged)-Human FANCD2/FANCI-associated nuclease 1 (FAN1), transcript variant 1

Product Images

Circular map for RC224249

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAN1
• Synonyms: hFAN1; KIAA1018; KMIN; MTMR15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224249 representing NM_014967
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTCAGAAGGGAAACCTCCTGACAAAAAAAGGCCTCGTAGAAGCTTATCAATCAGCAAGAATAAGA
  AAAAAGCATCTAATTCTATTATTTCGTGTTTTAACAATGCACCACCTGCTAAACTTGCCTGCCCCGTTTG
  CAGTAAAATGGTGCCTAGATATGACTTAAACCGGCACCTTGATGAAATGTGTGCTAACAATGACTTCGTT
  CAAGTGGATCCAGGGCAGGTTGGCTTAATAAATTCAAATGTGTCTATGGTAGATTTAACCAGTGTTACCT
  TAGAAGATGTAACACCTAAGAAGTCACCACCACCAAAGACAAATTTAACCCCTGGCCAAAGTGATTCAGC
  AAAAAGGGAAGTAAAGCAGAAGATCAGTCCCTACTTTAAAAGTAATGATGTGGTGTGCAAAAATCAAGAT
  GAGCTGAGAAATCGTAGTGTGAAAGTCATTTGTTTGGGAAGCCTAGCATCTAAATTGTCCAGAAAATACG
  TAAAGGCTAAAAAATCAATAGATAAGGATGAAGAATTTGCCGGTTCTAGTCCACAGAGTTCCAAATACAC
  AGTTGTTAAGAGCCTGATTGATAACTCTTCAGAAATTGAGGACGAGGATCAAATTTTGGAGAACAGTTCT
  CAAAAAGAAAACGTGTTTAAATGTGATTCTCTAAAGGAAGAGTGCATTCCTGAACATATGGTAAGAGAAA
  GTAAAATAATGGAAGCCGAAAGCCAAAAGGCTACCCGGGAATGTGAGAAATCAGCCCTCACCCCTGGATT
  CTCAGATAATGCGATCATGTTATTCTCACCAGATTTCACTCTTAGGAATACATTAAAGTCTACTTCAGAA
  GACAGTCTTGTAAAGCAAGAGTGTATCAAAGAAGTGGTTGAAAAACGTGAGGCATGTCATTGTGAAGAAG
  TAAAAATGACTGTTGCTTCAGAAGCTAAAATACAGCTGTCAGATTCAGAGGCAAAATCTCATAGTTCTGC
  AGATGATGCTTCTGCATGGAGTAACATCCAAGAGGCTCCTCTGCAGGATGACAGTTGCTTAAACAATGAT
  ATCCCTCACAGCATTCCTTTGGAGCAGGGGTCAAGCTGCAATGGTCCTGGTCAAACAACCGGTCATCCTT
  ACTACCTTCGGAGTTTCCTTGTGGTGCTGAAAACCGTACTTGAGAATGAAGATGATATGTTGCTCTTTGA
  TGAGCAGGAGAAGGGAATTGTAACTAAATTTTATCAGTTATCAGCTACTGGTCAGAAGTTATATGTAAGG
  CTCTTTCAACGTAAATTAAGCTGGATTAAGATGACCAAATTAGAGTATGAAGAGATTGCCTTAGACTTAA
  CACCTGTGATTGAAGAATTGACGAATGCAGGCTTTCTACAGACAGAATCTGAGTTGCAAGAACTCTCTGA
  AGTGCTTGAACTCCTTTCTGCTCCTGAACTAAAATCCCTAGCCAAGACCTTCCACTTGGTGAATCCCAAT
  GGACAGAAACAGCAGCTGGTGGACGCCTTTCTCAAATTGGCCAAACAGCGTTCAGTCTGCACTTGGGGCA
  AGAATAAGCCTGGAATTGGTGCAGTGATTTTAAAAAGAGCCAAAGCCTTGGCTGGACAGTCAGTACGAAT
  CTGTAAAGGCCCCAGGGCTGTGTTTTCCCGCATCTTGCTACTGTTTTCGTTGACCGACTCAATGGAAGAT
  GAAGACGCCGCTTGTGGAGGTCAGGGACAGCTTTCAACAGTCCTGTTGGTCAACCTCGGCCGAATGGAGT
  TTCCTAGTTACACCATCAATCGGAAAACCCACATCTTCCAAGACAGAGATGATCTTATCAGATATGCAGC
  AGCCACGCACATGCTGAGTGACATTTCTTCCGCAATGGCCAATGGGAACTGGGAAGAAGCTAAGGAGCTC
  GCTCAGTGTGCAAAAAGGGATTGGAACAGACTGAAAAACCACCCTTCTCTGAGATGCCACGAAGATTTAC
  CACTCTTCCTGCGGTGTTTCACTGTTGGGTGGATTTATACAAGGATTTTGTCTCGGTTTGTGGAAATACT
  GCAGAGACTTCACATGTATGAGGAAGCCGTCAGAGAACTTGAAAGCCTTTTGTCTCAGAGAATTTATTGT
  CCTGACAGCAGAGGCCGATGGTGGGATCGACTGGCCCTTAATTTACACCAGCACTTGAAGCGCCTGGAAC
  CGACTATCAAGTGCATCACAGAGGGGCTGGCGGATCCGGAAGTCAGAACGGGACACCGCCTTTCACTGTA
  TCAGCGAGCCGTGCGCCTGCGAGAGTCTCCGAGCTGTAAAAAGTTCAAGCACCTCTTCCAGCAGCTCCCA
  GAAATGGCTGTGCAAGATGTGAAACACGTGACCATCACAGGCAGGCTGTGCCCACAGCGTGGGATGTGCA
  AGTCTGTGTTTGTGATGGAGGCCGGGGAGGCCGCTGACCCCACCACGGTCCTGTGCTCTGTGGAGGAGCT
  GGCACTGGCCCATTACAGACGCAGCGGTTTTGACCAGGGGATTCATGGCGAAGGGTCCACCTTCAGCACC
  CTGTATGGCCTCCTCCTGTGGGACATCATCTTCATGGATGGGATTCCGGATGTCTTCAGAAACGCCTGTC
  AGGCATTCCCCCTGGACTTGTGCACAGACAGCTTCTTCACAAGCAGACGCCCAGCCCTTGAGGCCAGGCT
  GCAGCTGATTCATGATGCCCCCGAGGAGAGCCTGCGGGCCTGGGTGGCAGCCACGTGGCATGAGCAGGAA
  GGCAGAGTGGCTTCCCTTGTCAGCTGGGATCGCTTCACGTCTCTTCAGCAAGCTCAGGATCTTGTCTCCT
  GCCTGGGGGGCCCTGTGCTCAGTGGTGTGTGCAGGCACCTGGCTGCTGACTTTCGACACTGTCGAGGGGG
  CCTCCCCGACCTGGTGGTGTGGAACTCCCAGAGCCGTCACTTTAAGCTGGTGGAAGTTAAAGGCCCCAAT
  GATCGTCTTTCACATAAGCAGATGATCTGGCTGGCTGAACTGCAGAAGCTGGGGGCTGAAGTAGAAGTCT
  GCCACGTGGTTGCAGTTGGAGCTAAGAGCCAAAGCCTTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224249 representing NM_014967
  Red=Cloning site Green=Tags(s)
  
  MMSEGKPPDKKRPRRSLSISKNKKKASNSIISCFNNAPPAKLACPVCSKMVPRYDLNRHLDEMCANNDFV
  QVDPGQVGLINSNVSMVDLTSVTLEDVTPKKSPPPKTNLTPGQSDSAKREVKQKISPYFKSNDVVCKNQD
  ELRNRSVKVICLGSLASKLSRKYVKAKKSIDKDEEFAGSSPQSSKYTVVKSLIDNSSEIEDEDQILENSS
  QKENVFKCDSLKEECIPEHMVRESKIMEAESQKATRECEKSALTPGFSDNAIMLFSPDFTLRNTLKSTSE
  DSLVKQECIKEVVEKREACHCEEVKMTVASEAKIQLSDSEAKSHSSADDASAWSNIQEAPLQDDSCLNND
  IPHSIPLEQGSSCNGPGQTTGHPYYLRSFLVVLKTVLENEDDMLLFDEQEKGIVTKFYQLSATGQKLYVR
  LFQRKLSWIKMTKLEYEEIALDLTPVIEELTNAGFLQTESELQELSEVLELLSAPELKSLAKTFHLVNPN
  GQKQQLVDAFLKLAKQRSVCTWGKNKPGIGAVILKRAKALAGQSVRICKGPRAVFSRILLLFSLTDSMED
  EDAACGGQGQLSTVLLVNLGRMEFPSYTINRKTHIFQDRDDLIRYAAATHMLSDISSAMANGNWEEAKEL
  AQCAKRDWNRLKNHPSLRCHEDLPLFLRCFTVGWIYTRILSRFVEILQRLHMYEEAVRELESLLSQRIYC
  PDSRGRWWDRLALNLHQHLKRLEPTIKCITEGLADPEVRTGHRLSLYQRAVRLRESPSCKKFKHLFQQLP
  EMAVQDVKHVTITGRLCPQRGMCKSVFVMEAGEAADPTTVLCSVEELALAHYRRSGFDQGIHGEGSTFST
  LYGLLLWDIIFMDGIPDVFRNACQAFPLDLCTDSFFTSRRPALEARLQLIHDAPEESLRAWVAATWHEQE
  GRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRHLAADFRHCRGGLPDLVVWNSQSRHFKLVEVKGPN
  DRLSHKQMIWLAELQKLGAEVEVCHVVAVGAKSQSLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014967
• ORF Size: 3051 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014967.5
• RefSeq Size: 4835 bp
• RefSeq ORF: 3054 bp
• Locus ID: 22909
• UniProt ID: Q9Y2M0
• Cytogenetics: 15q13.3
• Domains: ZnF_Rad18
• MW: 114 kDa
• Gene Summary: This gene plays a role in DNA interstrand cross-link repair and encodes a protein with 5' flap endonuclease and 5'-3' exonuclease activity. Mutations in this gene cause karyomegalic interstitial nephritis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Feb 2016]